Detailed view for PBANKA_0824100

Basic information

TDR Targets ID: 828763
Plasmodium berghei, nitric oxide synthase, putative
Source Database / ID: 

pI: 9.3283 | Length (AA): 751 | MW (Da): 88038 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00175   Oxidoreductase NAD-binding domain
PF00258   Flavodoxin
PF00667   FAD binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016491   oxidoreductase activity  
GO:0010181   FMN binding  
GO:0055114   oxidation reduction  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
44 751 3qfc (B) 70 679 26.00 0 1 0.903943 0.81
57 751 2bpo (A) 59 691 28.00 0 1 1.06373 0.64
58 206 4oxx (A) 2 149 21.00 0 1 0.563602 -1.67
58 211 1bvy (F) 481 630 23.00 0 1 0.58326 -1.66
552 751 4bpr (A) 91 303 35.00 0.0000000094 1 0.569612 0.29

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Ookinete. Otto TD
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile 4 hs Ring, 16 hs Trophozoite, Erthyrocytic stages, Male gametocyte. Otto TD Yeoh LM
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Gametocyte, 22 hs Schizont, Female gametocyte. Otto TD Yeoh LM
Show/Hide expression data references
  • Otto TD A comprehensive evaluation of rodent malaria parasite genomes and gene expression.
  • Yeoh LM Comparative transcriptomics of female and male gametocytes in Plasmodium berghei and the evolution of sex in alveolates.

Orthologs

Ortholog group members (OG5_127162)

Species Accession Gene Product
Arabidopsis thaliana AT4G30210   NADPH--cytochrome P450 reductase 2
Arabidopsis thaliana AT4G24520   NADPH--cytochrome P450 reductase 1
Babesia bovis BBOV_I002330   flavodoxin and oxidoreductase NAD-binding domain containing protein
Brugia malayi Bm1_41510   FAD binding domain containing protein
Candida albicans CaO19.2672   NADP-cytochrome P450 reductase
Candida albicans CaO19.10187   NADP-cytochrome P450 reductase
Caenorhabditis elegans CELE_K10D2.6   Protein EMB-8
Cryptosporidium parvum cgd4_2700   probable NADPH-cytochrome p450 reductase
Chlamydia trachomatis CT_435   sulfite reductase
Dictyostelium discoideum DDB_G0269912   NADPH-cytochrome-P450 oxidoreductase
Dictyostelium discoideum DDB_G0293904   NADPH-cytochrome-P450 oxidoreductase
Drosophila melanogaster Dmel_CG11567   Cytochrome P450 reductase
Escherichia coli b2764   sulfite reductase, alpha subunit, flavoprotein
Echinococcus granulosus EgrG_000797900   NADPH cytochrome P450 reductase
Echinococcus multilocularis EmuJ_000797900   NADPH cytochrome P450 reductase
Giardia lamblia GL50803_15897   Hypothetical protein
Homo sapiens ENSG00000127948   P450 (cytochrome) oxidoreductase
Leishmania braziliensis LbrM.28.1340   p450 reductase, putative
Leishmania donovani LdBPK_281350.1   p450 reductase, putative
Leishmania infantum LinJ.28.1350   p450 reductase, putative
Leishmania major LmjF.28.1240   p450 reductase, putative
Leishmania mexicana LmxM.28.1240   p450 reductase, putative
Loa Loa (eye worm) LOAG_00956   FAD binding domain-containing protein
Mus musculus ENSMUSG00000005514   P450 (cytochrome) oxidoreductase
Mycobacterium tuberculosis Rv1394c   Probable cytochrome P450 132 Cyp132
Mycobacterium ulcerans MUL_1474   formate dehydrogenase H FdhF
Neospora caninum NCLIV_060990   hypothetical protein
Oryza sativa 4345047   Os08g0243500
Oryza sativa 4347838   Os09g0558900
Oryza sativa 4337244   Os04g0653400
Plasmodium berghei PBANKA_0824100   nitric oxide synthase, putative
Plasmodium falciparum PF3D7_0923200   nitric oxide synthase, putative
Plasmodium knowlesi PKNH_0721200   nitric oxide synthase, putative
Plasmodium vivax PVX_099570   NADPH-cytochrome p450 reductase, putative
Plasmodium yoelii PY05179   unnamed protein product-related
Saccharomyces cerevisiae YHR042W   Ncp1p
Schistosoma japonicum Sjp_0040140   ko:K00327 NADPH-ferrihemoprotein reductase [EC1.6.2.4], putative
Schistosoma japonicum Sjp_0207040   ko:K00327 NADPH-ferrihemoprotein reductase [EC1.6.2.4], putative
Schistosoma mansoni Smp_030760   cytochrome P450 reductase
Schmidtea mediterranea mk4.001376.10   NADPHALQ-cytochrome P450 reductase
Schmidtea mediterranea mk4.051841.00  
Schmidtea mediterranea mk4.003471.02  
Trypanosoma brucei gambiense Tbg972.11.9550   NADPH--cytochrome p450 reductase, putative
Trypanosoma brucei gambiense Tbg972.11.8610   NADPH--cytochrome p450 reductase, putative
Trypanosoma brucei Tb927.11.8380   NADPH--cytochrome P450 reductase, putative
Trypanosoma brucei Tb11.02.5420b   NADPH-cytochrome p450 reductase, putative
Trypanosoma congolense TcIL3000_0_54440   NADPH-cytochrome p450 reductase, putative
Trypanosoma cruzi TcCLB.506585.70   p450 reductase, putative
Toxoplasma gondii TGME49_219630   flavodoxin domain-containing protein
Theileria parva TP03_0092   hypothetical protein
Trichomonas vaginalis TVAG_136330   sulfite reductase, putative
Trichomonas vaginalis TVAG_483690   NADPH fad oxidoreductase, putative

Essentiality

PBANKA_0824100 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu1417 Mycobacterium tuberculosis non-essential nmpdr
Tb11.02.5420 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.02.5420 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.5420 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.5420 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.01.0170 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.0170 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.0170 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.0170 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2764 Escherichia coli non-essential goodall
CELE_K10D2.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_K10D2.6 Caenorhabditis elegans larval arrest wormbase
CELE_K10D2.6 Caenorhabditis elegans slow growth wormbase
CELE_K10D2.6 Caenorhabditis elegans sterile wormbase
YHR042W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0824100 this record Plasmodium berghei Essential plasmo
TGME49_219630 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PBANKA_0824100 (Plasmodium berghei), nitric oxide synthase, putative
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