Detailed view for TGME49_308810

Basic information

TDR Targets ID: 835163
Toxoplasma gondii, rhoptry neck protein RON9
Source Database / ID:  ToxoDB 

pI: 3.9896 | Length (AA): 1493 | MW (Da): 158583 | Paralog Number: 0

Signal peptide: N | GPI Anchor: Y | Predicted trans-membrane segments: 1

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00084   Sushi repeat (SCR repeat)
PF12796   Ankyrin repeats (3 copies)
PF13857   Ankyrin repeats (many copies)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 18 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
147 237 3tul (D) 109 225 25.00 0.57 0.02 0.487951 -2.27
151 1048 1st6 (A) 77 991 11.00 0 0.18 0.488674 -0.01
369 585 5cwi (A) 2 227 27.00 0.000012 1 0.574345 -1.41
369 599 5cwm (A) 2 226 19.00 0 0.19 0.254922 -1.35
621 743 5hmo (A) 813 914 31.00 0.24 0.05 0.345384 -1.6
1070 1373 5jhq (A) 193 475 34.00 0.00000000003 1 0.323617 0.68
1104 1305 2f8y (A) 1927 2111 31.00 0.000000002 1 0.464298 -0.21
1106 1249 1awc (B) 7 133 37.00 0.000000049 1 0.48545 -0.61
1120 1205 1a5e (A) 58 132 40.00 0.11 0.81 0.316602 0.7
1126 1240 3aaa (C) 10 117 34.00 0.0073 0.95 0.373026 0.07
1148 1261 1n0r (A) 2 109 48.00 0 1 0.667356 -0.73
1170 1352 1ot8 (A) 36 204 33.00 0.00000000059 1 0.420572 0.2
1181 1357 5aq8 (A) 13 171 39.00 0 1 0.520553 -0.34
1186 1296 3deo (A) 130 234 36.00 0.0001 1 0.529347 -0.77
1227 1375 4gmr (A) 11 145 44.00 0 1 0.545799 -0.18
1392 1469 2yra (A) 740 810 35.00 0.0069 1 0.363244 0.37
1407 1463 2xrb (A) 110 162 40.00 0.0068 0.66 0.266178 0.76
1432 1490 4lsz (E) 46 104 7.00 0.61 0.01 0.0667178 -0.24

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_159454)

Species Accession Gene Product
Cryptosporidium hominis Chro.40272   chain A, 4ank
Cryptosporidium parvum cgd4_2420   extracelllular membrane associated protein with a signal peptide, 5 ankyrin repeats, SUSHI domain and a transmembrane region
Neospora caninum NCLIV_053290   ORF73, related
Toxoplasma gondii TGME49_308810   rhoptry neck protein RON9

Essentiality

TGME49_308810 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_308810 this record Toxoplasma gondii Probably essential sidik
TGME49_308810 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0124 0.2899 0.998
0.0192 0.6597 0.5
0.0083 0.3992 1
0.0019 0.8576 0.5
0.021 0.8848 0.5
0.0372 0.3318 0.6696
0.0048 0.4805 0.5
0.0014 0.5 0.5
0.0122 0.2689 0.3097
0.007 0.2584 0.472
0.0019 0.3722 0.5
0.0122 0.3806 1
0.0064 0.7202 1
0.0051 0.4084 0.7391
0.0199 0.3328 0.6843
0.0177 0.2799 0
0.008 0.5777 1
0.0039 0.4087 0.356
0.0205 0.8878 1
0.0108 0.8207 1
0.0154 0.8389 1
0.0037 0.4313 0.3522
0.0183 0.8518 1
0.012 0.5678 1
0.0209 0.8673 0.5
0.017 0.4006 1
0.0053 0.2611 0.5
0.007 0.8608 1
0.021 0.8083 0.5
0.0028 0.6164 0.5
0.0192 0.884 1
0.0106 0.2824 0.5
0.014 0.3642 1
0.017659 0.79602 1
0.0195 0.4671 0.6828
0.007 0.8608 1
0.007 0.4947 1
0.0137 0.746 1
0.0019 0.8729 0.5
0.0137 0.8002 1
0.0175 0.5246 0.5
0.0079 0.8795 0.5
0.0141 0.8483 1
0.0014 0.4585 1
0.0193 0.8686 0.5
0.0105 0.5386 0.7538
0.0041 0.255 0
0.0139 0.747 1
0.0085 0.5135 1
0.0209 0.7657 1
0.0177 0.2799 0
0.0019 0.3471 0.5
0.0243 0.3132 0.6507
0.0135 0.7472 1
0.0201 0.8411 1
0.0014 0.5 0.5
0.0068 0.7089 1
0.0014 0.5 0.5
0.0176 0.7974 1
0.0083 0.381 1
0.0177 0.2799 0
0.0019 0.822 0.5
0.0173 0.5581 0.9848
0.0192 0.8659 1
0.0079 0.2683 1
0.0048 0.7359 0.6613
0.0039 0.409 0.3562
0.0039 0.6508 0.6359
0.0014 0.5 0.5
0.0149 0.3593 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_308810 (Toxoplasma gondii), rhoptry neck protein RON9
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