Detailed view for Rv2622

Basic information

TDR Targets ID: 8648
Mycobacterium tuberculosis, Possible methyltransferase (methylase)

Source Database / ID:  Tuberculist 

pI: 11.0328 | Length (AA): 273 | MW (Da): 29542 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF08241   Methyltransferase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008168   methyltransferase activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
14 203 3dr5 (A) 15 209 19.00 0 0.99 0.871871 -0.07
19 269 3bus (A) 25 273 24.00 0 1 1.10731 0.49
43 171 3bus (A) 49 182 37.00 0.0034 0.75 0.759027 0.47
54 155 1ve3 (A) 38 141 22.00 0.0000000000067 0.49 0.661826 -0.56

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_132295)

Species Accession Gene Product
Arabidopsis thaliana AT3G18000   phosphoethanolamine N-methyltransferase 1
Arabidopsis thaliana AT1G73600   putative phosphoethanolamine N-methyltransferase 3
Arabidopsis thaliana AT1G48600   phosphoethanolamine N-methyltransferase 2
Caenorhabditis elegans CELE_F54D11.1   Protein PMT-2
Dictyostelium discoideum DDB_G0275359   hypothetical protein
Mycobacterium tuberculosis Rv0469   Possible mycolic acid synthase UmaA
Mycobacterium tuberculosis Rv2622   Possible methyltransferase (methylase)
Mycobacterium ulcerans MUL_3266   methyltransferase
Mycobacterium ulcerans MUL_4538   mycolic acid synthase UmaA
Oryza sativa 4339516   Os05g0548900
Plasmodium falciparum PF3D7_1343000   phosphoethanolamine N-methyltransferase
Plasmodium knowlesi PKNH_1258400   phosphoethanolamine N-methyltransferase
Plasmodium vivax PVX_083045   phosphoethanolamine N-methyltransferase

Essentiality

Rv2622 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2668 this record Mycobacterium tuberculosis non-essential nmpdr
mtu473 Mycobacterium tuberculosis non-essential nmpdr
CELE_F54D11.1 Caenorhabditis elegans sterile wormbase
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Plasmodium falciparum phosphoethanolamine N-methyltransferase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.0139 0.5369 1
0.0152 0.8767 1
0.0159 0.2533 1
0.015 0.9384 1
0.0139 0.9381 1
0.0144 0.9384 1
0.0163 0.877 1
0.0134 0.3769 1
0.0137 0.9384 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

63 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Rv2622 (Mycobacterium tuberculosis), Possible methyltransferase (methylase)
Title for this comment
Comment