Detailed view for LOAG_08551

Basic information

TDR Targets ID: 946327
Loa Loa (eye worm), alanyl-tRNA synthetase
Source Database / ID:  KEGG  

pI: 7.6855 | Length (AA): 839 | MW (Da): 95028 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01411   tRNA synthetases class II (A)
PF07973   Threonyl and Alanyl tRNA synthetase second additional domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0005524   ATP binding  
GO:0004813   alanine-tRNA ligase activity  
GO:0004812   aminoacyl-tRNA ligase activity  
GO:0003676   nucleic acid binding  
GO:0000166   nucleotide binding  
GO:0043039   tRNA aminoacylation  
GO:0006419   alanyl-tRNA aminoacylation  

Metabolic Pathways

Aminoacyl-tRNA biosynthesis (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 839 3wqy (A) 37 818 15.00 0.00018 1 1.02331 1.16
22 456 5knn (A) 5 446 47.00 0 1 1.16797 -1.19

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126799)

Species Accession Gene Product
Arabidopsis thaliana AT1G50200   Alanyl-tRNA synthetase
Arabidopsis thaliana AT5G22800   probable alanine--tRNA ligase
Babesia bovis BBOV_III011650   alanyl-tRNA synthetase family protein
Brugia malayi Bm1_41830   Alanyl-tRNA synthetase
Brugia malayi Bm1_53535   alanyl-tRNA synthetase family protein
Candida albicans CaO19.5746   likely cytoplasmic alanyl-tRNA synthetase similar to S. cerevisiae ALA1 (YOR335C)
Candida albicans CaO19.13169   likely cytoplasmic alanyl-tRNA synthetase similar to S. cerevisiae ALA1 (YOR335C)
Caenorhabditis elegans CELE_F28H1.3   Protein AARS-2
Cryptosporidium hominis Chro.60075   cytosolic tRNA-Ala synthetase
Cryptosporidium parvum cgd6_560   alanyl-tRNA synthetase (with HxxxH domain)
Chlamydia trachomatis CT_749   alanine--tRNA ligase
Dictyostelium discoideum DDB_G0277823   alanine-tRNA ligase
Dictyostelium discoideum DDB_G0275655   alanine-tRNA ligase
Drosophila melanogaster Dmel_CG4633   mitochondrial alanyl-tRNA synthetase
Drosophila melanogaster Dmel_CG13391   Alanyl-tRNA synthetase
Escherichia coli b2697   alanyl-tRNA synthetase
Echinococcus granulosus EgrG_000437500   alanyl tRNA synthetase cytoplasmic
Entamoeba histolytica EHI_198790   alanyl-tRNA synthetase, putative
Echinococcus multilocularis EmuJ_000437500   alanyl tRNA synthetase, cytoplasmic
Giardia lamblia GL50803_96460   Alanyl-tRNA synthetase
Homo sapiens ENSG00000124608   alanyl-tRNA synthetase 2, mitochondrial
Homo sapiens ENSG00000090861   alanyl-tRNA synthetase
Leishmania braziliensis LbrM.22.1440   alanyl-tRNA synthetase, putative
Leishmania donovani LdBPK_221390.1   alanyl-tRNA synthetase, putative
Leishmania infantum LinJ.22.1390   alanyl-tRNA synthetase, putative
Leishmania major LmjF.22.1540   alanyl-tRNA synthetase, putative
Leishmania mexicana LmxM.22.1540   alanyl-tRNA synthetase, putative
Loa Loa (eye worm) LOAG_02129   alanyl-tRNA synthetase
Loa Loa (eye worm) LOAG_08551   alanyl-tRNA synthetase
Mycobacterium leprae ML0512   Probable alanyl-tRNA synthetase AlaS (Alanine--tRNA ligase) (Alanine translase) AlaRS)
Mus musculus ENSMUSG00000023938   alanyl-tRNA synthetase 2, mitochondrial (putative)
Mus musculus 234734   alanyl-tRNA synthetase
Mycobacterium tuberculosis Rv2555c   Probable alanyl-tRNA synthetase AlaS (alanine--tRNA ligase) (alanine translase) (ALARS)
Mycobacterium ulcerans MUL_1754   alanyl-tRNA synthetase
Neospora caninum NCLIV_061050   hypothetical protein
Oryza sativa 9266147   Os06g0245800
Oryza sativa 4348211   Os10g0182000
Plasmodium berghei PBANKA_1143600   alanine--tRNA ligase, putative
Plasmodium falciparum PF3D7_1367700   alanine--tRNA ligase
Plasmodium knowlesi PKNH_1103500   alanine--tRNA ligase, putative
Plasmodium vivax PVX_115345   alanine--tRNA ligase, putative
Plasmodium yoelii PY03081   alanyl-tRNA synthetase, putative
Saccharomyces cerevisiae YOR335C   alanine--tRNA ligase
Schistosoma japonicum Sjp_0075600   ko:K01872 alanyl-tRNA synthetase [EC6.1.1.7], putative
Schistosoma japonicum Sjp_0300520   ko:K01872 alanyl-tRNA synthetase [EC6.1.1.7], putative
Schistosoma mansoni Smp_038240   alanyl-tRNA synthetase
Schmidtea mediterranea mk4.039773.00  
Schmidtea mediterranea mk4.032719.04  
Schmidtea mediterranea mk4.000455.00   AlanineALQ-tRNA ligase, cytoplasmic
Schmidtea mediterranea mk4.002899.01   AlanineALQ-tRNA ligase, mitochondrial
Schmidtea mediterranea mk4.006608.04  
Trypanosoma brucei gambiense Tbg972.6.390   alanyl-tRNA synthetase, putative
Trypanosoma brucei Tb927.6.700   alanyl-tRNA synthetase, putative
Trypanosoma congolense TcIL3000_6_250   alanyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.506863.10   alanyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.511825.220   alanyl-tRNA synthetase, putative
Toxoplasma gondii TGME49_219540   cytosolic tRNA-Ala synthetase
Treponema pallidum TP1017   alanyl-tRNA synthetase
Theileria parva TP01_1004   alanyl-tRNA synthetase, putative
Trichomonas vaginalis TVAG_299450   alanyl-tRNA synthetase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0361   alanyl-tRNA synthetase

Essentiality

LOAG_08551 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.700 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.6.700 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.700 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.700 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b2697 Escherichia coli essential goodall
CELE_F28H1.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_F28H1.3 Caenorhabditis elegans larval arrest wormbase
CELE_F28H1.3 Caenorhabditis elegans slow growth wormbase
CELE_F28H1.3 Caenorhabditis elegans sterile wormbase
YOR335C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1143600 Plasmodium berghei Essential plasmo
TGME49_219540 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LOAG_08551 (Loa Loa (eye worm)), alanyl-tRNA synthetase
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