Detailed view for LOAG_11686

Basic information

TDR Targets ID: 946987
Loa Loa (eye worm), biotin protein ligase 1
Source Database / ID:  KEGG  

pI: 7.6875 | Length (AA): 367 | MW (Da): 41630 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03099   Biotin/lipoate A/B protein ligase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0004077   biotin-[acetyl-CoA-carboxylase] ligase activity  
GO:0006464   protein modification process  

Metabolic Pathways

Biotin metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
150 353 4xtv (A) 19 218 18.00 0 0.99 0.819758 -0.85
152 343 2dxu (B) 4 179 24.00 0 0.99 0.841061 -0.88
152 301 2dzc (A) 4 137 38.00 0.000011 0.85 0.550319 0.46

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126858)

Species Accession Gene Product
Arabidopsis thaliana AT1G37150   holocarboxylase synthetase 2
Arabidopsis thaliana AT2G25710   holocarboxylase synthase 1
Brugia malayi Bm1_15240   biotin--acetyl-CoA-carboxylase ligase family protein
Candida albicans CaO19.7645   Biotin:apoprotein ligase
Caenorhabditis elegans CELE_F13H8.10   Protein BPL-1, isoform C
Cryptosporidium hominis Chro.20303   transcriptional repressor of the biotin operon birA
Cryptosporidium parvum cgd2_2890   putative biotin-(acetyl-CoA carboxylase) ligase
Chlamydia trachomatis CT_035   hypothetical protein
Chlamydia trachomatis CT_725   biotin synthetase
Dictyostelium discoideum DDB_G0289035   biotin--[acetyl-CoA-carboxylase] ligase 3
Dictyostelium discoideum DDB_G0288789   biotin--[acetyl-CoA-carboxylase] ligase 2
Dictyostelium discoideum DDB_G0288791   biotin--[acetyl-CoA-carboxylase] ligase 1
Drosophila melanogaster Dmel_CG14670   Holocarboxylase synthetase
Escherichia coli b3973   bifunctional biotin-[acetylCoA carboxylase] holoenzyme synthetase/DNA-binding transcriptional repressor, bio-5'-AMP-binding
Echinococcus granulosus EgrG_000518400   5'partial|biotin protein ligase
Echinococcus granulosus EgrG_000685000   N terminal of biotin protein ligase
Entamoeba histolytica EHI_104900   hypothetical protein, conserved
Entamoeba histolytica EHI_167730   biotin--acetyl-CoA-carboxylase ligase, putative
Entamoeba histolytica EHI_193800   hypothetical protein, conserved
Entamoeba histolytica EHI_033910   hypothetical protein, conserved
Entamoeba histolytica EHI_011260   hypothetical protein, conserved
Entamoeba histolytica EHI_101430   hypothetical protein, conserved
Entamoeba histolytica EHI_193790   hypothetical protein, conserved
Entamoeba histolytica EHI_104890   hypothetical protein, conserved
Entamoeba histolytica EHI_177040   hypothetical protein, conserved
Entamoeba histolytica EHI_101420   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000518400   biotin protein ligase
Echinococcus multilocularis EmuJ_000685000   N terminal of biotin protein ligase
Echinococcus multilocularis EmuJ_000518600   biotin protein ligase
Giardia lamblia GL50803_12082   Hypothetical protein
Homo sapiens ENSG00000159267   holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase)
Leishmania braziliensis LbrM.31.1280   biotin/lipoate protein ligase-like protein
Leishmania donovani LdBPK_311070.1   biotin/lipoate protein ligase-like protein
Leishmania infantum LinJ.31.1070   biotin/lipoate protein ligase-like protein
Leishmania major LmjF.31.1070   biotin/lipoate protein ligase-like protein
Leishmania mexicana LmxM.30.1070   biotin/lipoate protein ligase-like protein
Loa Loa (eye worm) LOAG_11686   biotin protein ligase 1
Loa Loa (eye worm) LOAG_15956   hypothetical protein
Loa Loa (eye worm) LOAG_10903   hypothetical protein
Mycobacterium leprae ML0732   POSSIBLE BIFUNCTIONAL PROTEIN BirA: BIOTIN OPERON REPRESSOR + BIOTIN--[ACETYL-COA-CARBOXYLASE] SYNTHETASE (BIOTIN--PROTEIN LIGAS
Mus musculus ENSMUSG00000040820   holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)
Mycobacterium tuberculosis Rv3279c   Possible bifunctional protein BirA: biotin operon repressor + biotin--[acetyl-CoA-carboxylase] synthetase (biotin--protein ligas
Mycobacterium ulcerans MUL_2631   bifunctional protein BirA
Oryza sativa 4328417   Os02g0167000
Plasmodium berghei PBANKA_1323600   biotin protein ligase, putative
Plasmodium berghei PBANKA_0511000   biotin--acetyl-CoA-carboxylase, putative
Plasmodium falciparum PF3D7_1460000   biotin protein ligase, putative
Plasmodium falciparum PF3D7_1026900   biotin--acetyl-CoA-carboxylase, putative
Plasmodium knowlesi PKNH_0611300   Biotin--acetyl-CoA-carboxylase ligase, putative
Plasmodium vivax PVX_111375   biotin--[acetyl-CoA-carboxylase] synthetase, putative
Plasmodium yoelii PY06060   biotin--acetyl-CoA-carboxylase ligase
Plasmodium yoelii PY01917   biotin--acetyl-CoA-carboxylase ligase
Saccharomyces cerevisiae YDL141W   biotin--[acetyl-CoA-carboxylase] ligase BPL1
Schistosoma japonicum Sjp_0021510   ko:K01942 biotin-[methylmalonyl-CoA-carboxyltransferase] ligase [EC6.3.4.9], putative
Schistosoma japonicum Sjp_0309010   expressed protein
Schistosoma japonicum Sjp_0021920   ko:K01942 biotin-[methylmalonyl-CoA-carboxyltransferase] ligase [EC6.3.4.9], putative
Schistosoma mansoni Smp_081420   biotin-protein ligase
Schistosoma mansoni Smp_153150   biotin-protein ligase
Schmidtea mediterranea mk4.000351.05   BiotinALQ-protein ligase
Trypanosoma brucei gambiense Tbg972.11.11300   Biotin--acetyl-CoA-carboxylase ligase, putative,biotin--protein ligase, putative
Trypanosoma brucei Tb927.11.10060   Biotin--acetyl-CoA-carboxylase ligase, putative
Trypanosoma congolense TcIL3000.11.10550   Biotin--acetyl-CoA-carboxylase ligase, putative
Trypanosoma congolense TcIL3000_0_32190   Biotin--acetyl-CoA-carboxylase ligase, putative
Trypanosoma cruzi TcCLB.510719.60   Biotin--acetyl-CoA-carboxylase ligase, putative
Trypanosoma cruzi TcCLB.509749.10   Biotin--acetyl-CoA-carboxylase ligase, putative
Treponema pallidum TP0357   biotin--acetyl-CoA-carboxylase ligase
Wolbachia endosymbiont of Brugia malayi Wbm0620   biotin-(acetyl-CoA carboxylase) ligase

Essentiality

LOAG_11686 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.1820 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.1820 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.1820 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.1820 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b3973 Escherichia coli essential goodall
YDL141W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0511000 Plasmodium berghei Dispensable plasmo
PBANKA_1323600 Plasmodium berghei Dispensable plasmo
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Mycobacterium tuberculosis Possible bifunctional protein BirA: biotin operon repressor + biotin--[acetyl-CoA-carboxylase] synthetase (biotin--protein ligas Compounds References
Staphylococcus aureus Bifunctional ligase/repressor BirA Compounds References
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) BirA bifunctional protein Compounds References
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) Biotin--acetyl-CoA-carboxylase ligase Compounds References
Homo sapiens holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) Compounds References
Escherichia coli bifunctional biotin-[acetylCoA carboxylase] holoenzyme synthetase/DNA-binding transcriptional repressor, bio-5'-AMP-binding Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.1154 0.4595 1
0.0359 0.3327 0.3351
0.0205 0.3921 0.4856
0.1351 0.4444 1
0.1305 0.4655 1
0.1163 0.2684 0.2684
0.1163 0.2684 0.2684
0.039 0.4444 1
0.039 0.4444 1
0.1106 0.4627 1
0.0812 0.4662 1
0.02 0.4598 0.8055
0.11 0.386 1
0.11 0.386 1
0.123 0.4657 1
0.0812 0.4657 1
0.0563 0.3443 1
0.0759 0.4444 1
0.0907 0.4654 1
0.0359 0.3327 0.3351
0.0182 0.3835 1
0.0205 0.3921 0.4856
0.1159 0.4365 1
0.0178 0.4444 0.4934
0.0141 0.4116 0.5967

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LOAG_11686 (Loa Loa (eye worm)), biotin protein ligase 1
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