Detailed view for LOAG_07505

Basic information

TDR Targets ID: 948707
Loa Loa (eye worm), ThiF family protein
Source Database / ID:  KEGG  

pI: 6.0237 | Length (AA): 625 | MW (Da): 70410 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00899   ThiF family
PF10585   Ubiquitin-activating enzyme active site
PF14732   Ubiquitin/SUMO-activating enzyme ubiquitin-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0019948   SUMO activating enzyme activity  
GO:0016925   protein sumoylation  
GO:0008641   small protein activating enzyme activity  

Metabolic Pathways

Ubiquitin mediated proteolysis (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
13 558 3kyc (B) 19 620 47.00 0 1 1.3909 -0.23
159 380 2px9 (A) 166 382 46.00 0 1 0.8555 -0.86

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127838)

Species Accession Gene Product
Arabidopsis thaliana AT2G21470   SUMO-activating enzyme subunit 2
Babesia bovis BBOV_IV001050   ubiquitin-activating enzyme, putative
Brugia malayi Bm1_53850   ThiF family protein
Candida albicans CaO19.5074   similar to Uba1 (Ub-activating enzyme)
Candida albicans CaO19.12540   similar to Uba1 (Ub-activating enzyme)
Cryptosporidium hominis Chro.20160   SUMO-1 activating enzyme subunit 2
Cryptosporidium parvum cgd2_1460   SUMO-1 activating enzyme subunit 2, putative
Dictyostelium discoideum DDB_G0286919   sumo-activating enzyme subunit 2
Drosophila melanogaster Dmel_CG7528   Smt3 activating enzyme 2
Echinococcus granulosus EgrG_001145300   sumo-activating enzyme subunit 2
Entamoeba histolytica EHI_035540   ubiquitin-activating enzyme, putative
Echinococcus multilocularis EmuJ_001145300   SUMO activating enzyme subunit 2
Giardia lamblia GL50803_6288   Ubiquitin-activating enzyme E1 1
Homo sapiens ENSG00000126261   ubiquitin-like modifier activating enzyme 2
Leishmania braziliensis LbrM.08.0230   ubiquitin-activating enzyme-like protein
Leishmania donovani LdBPK_080230.1   ubiquitin-activating enzyme-like protein
Leishmania infantum LinJ.08.0230   ubiquitin-activating enzyme-like protein
Leishmania major LmjF.08.0220   ubiquitin-activating enzyme-like protein
Leishmania mexicana LmxM.08.0220   ubiquitin-activating enzyme-like protein
Loa Loa (eye worm) LOAG_07505   ThiF family protein
Mus musculus ENSMUSG00000052997   ubiquitin-like modifier activating enzyme 2
Neospora caninum NCLIV_055390   AFR138Wp, related
Oryza sativa 4343750   Os07g0586500
Plasmodium berghei PBANKA_1451600   SUMO-activating enzyme subunit 2, putative
Plasmodium falciparum PF3D7_1237000   SUMO-activating enzyme subunit 2
Plasmodium knowlesi PKNH_1456700   SUMO-activating enzyme subunit 2, putative
Plasmodium vivax PVX_100800   SUMO-activating enzyme subunit 2, putative
Plasmodium yoelii PY05539   related to ubiquitin-activating enzyme homolog UBA2-related
Saccharomyces cerevisiae YDR390C   E1 ubiquitin-activating protein UBA2
Schistosoma japonicum Sjp_0101860   ko:K10685 ubiquitin-like 1-activating enzyme E1 B, putative
Schistosoma mansoni Smp_166220   ubiquitin-activating enzyme E1b
Schmidtea mediterranea mk4.026733.00   SUMO-activating enzyme subunit uba-2
Schmidtea mediterranea mk4.002146.08   SUMO-activating enzyme subunit uba-2
Schmidtea mediterranea mk4.003668.01   SUMO-activating enzyme subunit 2
Schmidtea mediterranea mk4.000051.00   SUMO-activating enzyme subunit uba-2
Schmidtea mediterranea mk4.021673.00   SUMO-activating enzyme subunit uba-2
Schmidtea mediterranea mk4.015346.00   SUMO-activating enzyme subunit uba-2
Schmidtea mediterranea mk4.002412.00   SUMO-activating enzyme subunit uba-2
Schmidtea mediterranea mk4.016507.01  
Schmidtea mediterranea mk4.000092.00   SUMO-activating enzyme subunit uba-2
Trypanosoma brucei gambiense Tbg972.5.4810   ubiquitin-activating enzyme e1, putative
Trypanosoma brucei Tb927.5.3430   ubiquitin-activating enzyme E1, putative
Trypanosoma congolense TcIL3000_5_3890   ubiquitin-activating enzyme E1, putative
Trypanosoma cruzi TcCLB.509117.10   ubiquitin-activating enzyme, putative
Trypanosoma cruzi TcCLB.511655.69   ubiquitin-activating enzyme-like protein
Trypanosoma cruzi TcCLB.509777.100   ubiquitin-activating enzyme, putative
Toxoplasma gondii TGME49_311500   ThiF family protein
Theileria parva TP01_0127   ubiquitin-protein ligase, putative
Trichomonas vaginalis TVAG_211530   molybdopterin biosynthesis moeb protein, putative

Essentiality

LOAG_07505 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.3430 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.5.3430 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.3430 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.5.3430 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
YDR390C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1451600 Plasmodium berghei Essential plasmo
TGME49_311500 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens ubiquitin-like modifier activating enzyme 2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0038 0.5276 0.4593
0.0057 1 0.5
0.0038 0.4012 0.2586

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LOAG_07505 (Loa Loa (eye worm)), ThiF family protein
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