Detailed view for EmuJ_000777100

Basic information

TDR Targets ID: 956618
Echinococcus multilocularis, aspartyl tRNA synthetase, cytoplasmic
Source Database / ID:  GeneDB

pI: 6.3649 | Length (AA): 514 | MW (Da): 58340 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00152   tRNA synthetases class II (D, K and N)
PF01336   OB-fold nucleic acid binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0005524   ATP binding  
GO:0004815   aspartate-tRNA ligase activity  
GO:0004812   aminoacyl-tRNA ligase activity  
GO:0003676   nucleic acid binding  
GO:0000166   nucleotide binding  
GO:0006422   aspartyl-tRNA aminoacylation  
GO:0006418   tRNA aminoacylation for protein translation  

Metabolic Pathways

Aminoacyl-tRNA biosynthesis (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 74 5hmo (A) 818 888 13.00 0.96 0.01 0.155032 -0.06
25 514 3i7f (A) 11 544 47.00 0 1 1.42431 -0.03
35 508 4j15 (A) 22 495 62.00 0 1 1.55428 -0.25
38 514 1eov (A) 71 557 54.00 0 1 1.57212 -0.37
338 473 1vlg (A) 9 144 15.00 0 0.1 0.410591 -0.56

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127227)

Species Accession Gene Product
Arabidopsis thaliana AT4G31180   Asx tRNA synthetase (AspRS/AsnRS) class II core domain-contating protein
Arabidopsis thaliana AT4G26870   Asx tRNA synthetase (AspRS/AsnRS) class II core domain-contating protein
Babesia bovis BBOV_IV001690   aspartyl-tRNA synthetase, putative
Brugia malayi Bm1_41230   Aspartyl-tRNA synthetase
Candida albicans CaO19.9945   probable aspartate-tRNA synthetase similar to S. cerevisiae DPS1 (YLL018C) cytoplasmic enzyme
Candida albicans CaO19.2407   probable aspartate-tRNA synthetase similar to S. cerevisiae DPS1 (YLL018C) cytoplasmic enzyme
Caenorhabditis elegans CELE_B0464.1   Protein DARS-1
Cryptosporidium hominis Chro.70181   aspartate--tRNA ligase
Cryptosporidium parvum cgd7_1540   aspartate--tRNA ligase
Dictyostelium discoideum DDB_G0272236   aspartate-tRNA ligase
Drosophila melanogaster Dmel_CG3821   Aspartyl-tRNA synthetase
Echinococcus granulosus EgrG_000777100   aspartyl tRNA synthetase cytoplasmic
Entamoeba histolytica EHI_175050   aspartyl-tRNA synthetase, putative
Echinococcus multilocularis EmuJ_000777100   aspartyl tRNA synthetase, cytoplasmic
Giardia lamblia GL50803_10870   Aspartyl-tRNA synthetase
Homo sapiens ENSG00000115866   aspartyl-tRNA synthetase
Leishmania braziliensis LbrM.30.0540   aspartyl-tRNA synthetase, putative
Leishmania donovani LdBPK_300470.1   aspartyl-tRNA synthetase, putative
Leishmania infantum LinJ.30.0470   aspartyl-tRNA synthetase, putative
Leishmania major LmjF.30.0460   aspartyl-tRNA synthetase, putative
Leishmania mexicana LmxM.29.0460   aspartyl-tRNA synthetase, putative
Loa Loa (eye worm) LOAG_11036   aspartyl-tRNA synthetase
Mus musculus ENSMUSG00000026356   aspartyl-tRNA synthetase
Neospora caninum NCLIV_022400   hypothetical protein
Oryza sativa 4328255   Os02g0139600
Oryza sativa 4330354   Os02g0686400
Plasmodium berghei PBANKA_0210200   aspartate--tRNA ligase, putative
Plasmodium falciparum PF3D7_0102900   aspartate--tRNA ligase
Plasmodium knowlesi PKNH_0210700   aspartate--tRNA ligase, putative
Plasmodium vivax PVX_081610   aspartate--tRNA ligase, putative
Plasmodium yoelii PY01511   aspartyl-tRNA synthetase, putative
Saccharomyces cerevisiae YLL018C   aspartate--tRNA ligase DPS1
Schistosoma japonicum Sjp_0051950   ko:K01876 aspartyl-tRNA synthetase [EC6.1.1.12], putative
Schistosoma mansoni Smp_053510.2   aspartyl-tRNA synthetase
Schistosoma mansoni Smp_053510.1   aspartyl-tRNA synthetase
Schmidtea mediterranea mk4.000319.04   AspartateALQ-tRNA ligase, cytoplasmic
Trypanosoma brucei gambiense Tbg972.6.1580   aspartyl-tRNA synthetase, putative
Trypanosoma brucei Tb927.6.1880   aspartyl-tRNA synthetase, putative
Trypanosoma congolense TcIL3000_6_1480   aspartyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.509037.50   aspartyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.510777.20   aspartyl-tRNA synthetase, putative
Toxoplasma gondii TGME49_202530   aspartate-tRNA ligase
Theileria parva TP03_0489   aspartyl-tRNA synthetase, putative
Trichomonas vaginalis TVAG_267950   lysyl-tRNA synthetase, putative

Essentiality

EmuJ_000777100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.1880 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.1880 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.1880 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.1880 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_B0464.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_B0464.1 Caenorhabditis elegans larval arrest wormbase
CELE_B0464.1 Caenorhabditis elegans slow growth wormbase
CELE_B0464.1 Caenorhabditis elegans sterile wormbase
YLL018C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0210200 Plasmodium berghei Essential plasmo
TGME49_202530 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier EmuJ_000777100 (Echinococcus multilocularis), aspartyl tRNA synthetase, cytoplasmic
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