Detailed view for EmuJ_000972000

Basic information

TDR Targets ID: 962201
Echinococcus multilocularis, methionyl tRNA synthetase, cytoplasmic
Source Database / ID:  GeneDB

pI: 7.6753 | Length (AA): 819 | MW (Da): 92774 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF08264   Anticodon-binding domain of tRNA
PF09334   tRNA synthetases class I (M)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0005524   ATP binding  
GO:0004825   methionine-tRNA ligase activity  
GO:0004812   aminoacyl-tRNA ligase activity  
GO:0000166   nucleotide binding  
GO:0006431   methionyl-tRNA aminoacylation  
GO:0006418   tRNA aminoacylation for protein translation  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 182 4bvx (A) 1 203 25.00 0 1 0.367701 0
3 161 4bvx (A) 2 185 33.00 0.0013 0.99 0.434339 -0.27
4 167 5jpo (A) 6 209 16.00 0.24 1 0.240944 -0.34
214 817 5gl7 (A) 229 819 58.00 0 1 1.51118 -1.27

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127626)

Species Accession Gene Product
Arabidopsis thaliana AT4G13780   probable methionine--tRNA ligase
Babesia bovis BBOV_IV006630   methionyl-tRNA synthetase, putative
Brugia malayi Bm1_12165   methionyl-tRNA synthetase
Candida albicans CaO19.3955   likely tRNA synthetase similar to S. cerevisiae MES1 (YGR264C) cytoplasmic methionyl tRNA synthetase
Candida albicans CaO19.11437   likely tRNA synthetase similar to S. cerevisiae MES1 (YGR264C) cytoplasmic methionyl tRNA synthetase
Caenorhabditis elegans CELE_F58B3.5   Protein MARS-1, isoform B
Chlamydia trachomatis CT_032   methionine--tRNA ligase
Dictyostelium discoideum DDB_G0279113   methionine-tRNA ligase
Drosophila melanogaster Dmel_CG15100   CG15100 gene product from transcript CG15100-RA
Escherichia coli b2114   methionyl-tRNA synthetase
Echinococcus granulosus EgrG_000972000   methionyl tRNA synthetase cytoplasmic
Entamoeba histolytica EHI_079860   methionyl-tRNA synthetase, putative
Echinococcus multilocularis EmuJ_000972000   methionyl tRNA synthetase, cytoplasmic
Homo sapiens ENSG00000166986   methionyl-tRNA synthetase
Loa Loa (eye worm) LOAG_04595   hypothetical protein
Loa Loa (eye worm) LOAG_04596   multisynthetase complex auxiliary component p43
Mus musculus ENSMUSG00000040354   methionine-tRNA synthetase
Oryza sativa 4341140   Os06g0508700
Oryza sativa 4348562   Os10g0400100
Saccharomyces cerevisiae YGR264C   methionine--tRNA ligase MES1
Schistosoma japonicum Sjp_0048360   Methionyl-tRNA synthetase, cytoplasmic, putative
Schistosoma japonicum Sjp_0105270   ko:K01874 methionyl-tRNA synthetase [EC6.1.1.10], putative
Schistosoma mansoni Smp_040770   methionine-tRNA synthetase
Schmidtea mediterranea mk4.002973.01  
Schmidtea mediterranea mk4.034972.04  
Schmidtea mediterranea mk4.002973.00   Putative methionine-tRNA synthetase
Schmidtea mediterranea mk4.000849.02   Putative methionine-tRNA synthetase
Treponema pallidum TP0798   methionyl-tRNA synthetase

Essentiality

EmuJ_000972000 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b2114 Escherichia coli essential goodall
CELE_F58B3.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_F58B3.5 Caenorhabditis elegans larval arrest wormbase
CELE_F58B3.5 Caenorhabditis elegans slow growth wormbase
YGR264C Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) Methionyl-tRNA synthetase Compounds References
Homo sapiens methionyl-tRNA synthetase Compounds References
Escherichia coli methionyl-tRNA synthetase Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Staphylococcus aureus subsp. aureus MW2 Methionyl-tRNA synthetase 657 aa 25.1% 561 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.027 1 1
0.0186 0.899 1
0.0106 0.8034 0.5
0.0053 0.324 0.324
0.0802 0.8034 0.5
0.0246876 0.308753 0.5
0.0276 0.3186 0.3186
0.0066 1 1
0.0158 0.9033 1
0.014 1 1
0.0482895 0.886309 1
0.0275 1 1
0.027 0.3129 0.3119
0.4092 0.5422 0.5421
0.4092 0.5422 0.5421
0.0046 0.3623 0
0.0379 0.5055 0.5055
0.0135 1 1
0.014 1 1
0.0161 0.9008 1
0.0596 0.8491 1
0.027 1 1
0.008 1 1
0.0133 1 1
0.0655 0.3178 0.685
0.0212 0.6599 0.6599
0.0466 0.2804 0.2804
0.0287 0.9376 1
0.0466 0.2804 0.2804
0.0198 0.8849 1
0.0145 1 1
0.0106 0.8347 1
0.0784 0.8544 0.9985
0.0774 1 1
0.0066 1 1
0.0202 0.2882 0
0.008 0.8674 1
0.0462 0.8263 1
0.0405 1 1
0.0281 0.3225 0.3097
0.0126 0.7986 1
0.0821 0.8545 1
0.0106 0.8347 1
0.0284 0.5095 0.5095
0.0337 0.3574 0.6662
0.0164 0.541 1
0.0049 0.2985 0.2947
0.0276 0.6202 0.3181
0.0265 0.9275 1
0.0907 0.9219 0.9215
0.0276 0.3186 0.3186
0.0106 0.8264 1
0.008 0.3601 0.3601
0.0632 0.8046 0.8046
0.0106 0.8034 0.5
0.008 1 1
0.0106 0.5947 1
0.0167 0.9008 1
0.0201 0.8353 1
0.0171 0.9008 1
0.0157 0.9008 1
0.0549 0.8281 1
0.008 1 1
0.0126 0.3003 0.4174
0.0172 0.8822 1
0.0155 0.9442 1
0.084 0.7738 1
0.0106 0.3032 0.2896
0.0276 0.5542 0.5508
0.0087 0.7451 0
0.0106 0.8034 0.5
0.0158 0.8876 1
0.027 1 1
0.0352 1 1
0.0222 0.3431 0.3431
0.0451 0.2875 1
0.0330347 0.416691 1
0.084 0.7738 1
0.0198 1 1
0.0649 0.8364 1
0.0284 0.5095 0.5095
0.0106 0.5702 0
0.0195 0.5759 0.5
0.0135 1 1
0.0269 0.2895 0.2892
0.008 0.2911 0.2911
0.0106 0.2764 0.2727
0.0484971 0.88635 1
0.0106 0.3564 0
0.008 0.5762 1
0.0164 0.541 1
0.0264 0.3205 0.3205
0.0228 0.6465 0.6465
0.0143 0.3501 1
0.0106 0.2764 0.2727
0.0106 0.5321 0

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier EmuJ_000972000 (Echinococcus multilocularis), methionyl tRNA synthetase, cytoplasmic
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