Detailed view for Rv3677c
Basic information
Mycobacterium tuberculosis, Possible hydrolase
Source Database / ID: Tuberculist
pI: 4.5469 |
Length (AA): 264 |
MW (Da): 28490 |
Paralog Number:
0
Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0
Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable
Network
Pfam domains
Gene Ontology
Metabolic Pathways
Structural information
Modbase 3D models:
There are 5 models calculated for this protein. More info on
these models, including the
models themselves is available at:
Modbase
| Target Beg | Target End | Template | Template Beg | Template End | Identity | Evalue | Model Score | MPQS | zDope |
|---|---|---|---|---|---|---|---|---|---|
| 9 | 264 | 5i0p (A) | 15 | 340 | 24.00 | 0 | 1 | 1.0531 | -0.13 |
| 15 | 264 | 4ad9 (A) | 7 | 271 | 29.00 | 0 | 1 | 1.27537 | -0.68 |
| 27 | 195 | 3kns (B) | 21 | 212 | 23.00 | 0 | 1 | 0.943552 | -1.03 |
| 51 | 181 | 1qh5 (A) | 24 | 163 | 34.00 | 0.000037 | 1 | 0.888912 | -0.61 |
| 65 | 224 | 6ao1 (A) | 128 | 300 | 36.00 | 0.000054 | 1 | 0.932761 | 0.25 |
Help me make sense of these data.
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)
A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.
PDB Structures:
Expression
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Mid 40-60% percentile | Dormant phase. | hasan |
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Lower 0-20% percentile | Dormant phase. | murphy |
-
hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis. -
murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
Orthologs
Ortholog group members (OG5_129461)
| Species | Accession | Gene Product |
|---|---|---|
| Arabidopsis thaliana | AT1G25375 | Metallo-hydrolase/oxidoreductase superfamily protein |
| Brugia malayi | Bm1_05545 | lactamase, beta 2 |
| Caenorhabditis elegans | CELE_Y53F4B.39 | Protein Y53F4B.39, isoform A |
| Dictyostelium discoideum | DDB_G0278253 | hypothetical protein |
| Drosophila melanogaster | Dmel_CG12375 | CG12375 gene product from transcript CG12375-RA |
| Echinococcus granulosus | EgrG_001111100 | metallo beta lactamase domain containing protein |
| Echinococcus multilocularis | EmuJ_001111100 | metallo beta lactamase domain containing protein |
| Homo sapiens | ENSG00000147592 | lactamase, beta 2 |
| Leishmania braziliensis | LbrM.21.0730 | metallo-beta-lactamase family-like protein |
| Leishmania donovani | LdBPK_210730.1 | metallo-beta-lactamase family-like protein |
| Leishmania infantum | LinJ.21.0730 | metallo-beta-lactamase family-like protein |
| Leishmania major | LmjF.21.0670 | metallo-beta-lactamase family-like protein |
| Leishmania mexicana | LmxM.21.0670 | metallo-beta-lactamase family-like protein |
| Loa Loa (eye worm) | LOAG_03054 | hypothetical protein |
| Mycobacterium leprae | ML2303c | POSSIBLE HYDROLASE |
| Mus musculus | 212442 | lactamase, beta 2 |
| Mycobacterium tuberculosis | Rv3677c | Possible hydrolase |
| Mycobacterium ulcerans | MUL_4251 | hypothetical protein |
| Oryza sativa | 4325805 | Os01g0133500 |
Essentiality
| Gene/Ortholog | Organism | Phenotype | Source Study |
|---|---|---|---|
| mtu3739 this record | Mycobacterium tuberculosis | non-essential | nmpdr |
-
blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930 -
shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan -
neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs -
wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170 -
keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection -
gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84 -
nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource -
yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database -
alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
Phenotypes and Validation (curated)
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
In any case, if you have information about papers containing relevant validation data for this target, please contact us.
Annotated validation
Associated compounds / Druggability
Druggability index (range: 0 to 1): 0.3
Known modulators for this target
Predicted associations
By orthology with druggable targets
By sequence similarity to non orthologous druggable targets
Obtained from network model
Ranking Plot
Putative Drugs List
Assayability
Assay information
Reagent availability
Bibliographic References