Detailed view for Rv0318c

Basic information

TDR Targets ID: 980439
Mycobacterium tuberculosis, Probable conserved integral membrane protein
Source Database / ID:  Tuberculist 

pI: 5.635 | Length (AA): 264 | MW (Da): 27208 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 8

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02535  

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  
GO:0046873   metal ion transmembrane transporter activity  
GO:0055085   transmembrane transport  
GO:0030001   metal ion transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
30 261 5tsa (A) 59 305 24.00 0.000053 0.65 1.16359 -0.4
40 257 5tsa (A) 69 301 31.00 0.00000037 0.97 1.06556 0.29
171 241 1x31 (C) 21 95 38.00 0.25 0.4 0.414739 1.86

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127397)

Species Accession Gene Product
Arabidopsis thaliana AT3G20870   zinc transporter ZTP29
Arabidopsis thaliana AT3G08650   putative zinc transporter
Brugia malayi Bm1_11530   ZIP Zinc transporter family protein
Candida albicans CaO19.9109   potential zinc-iron permease similar to S. cerevisiae ZRT3 (YKL175W)
Candida albicans CaO19.1534   potential zinc-iron permease similar to S. cerevisiae ZRT3 (YKL175W)
Caenorhabditis elegans CELE_F59A3.4   Protein F59A3.4
Dictyostelium discoideum DDB_G0286345   zinc transporter
Drosophila melanogaster Dmel_CG13189   Zinc/iron regulated transporter-related protein 48C
Escherichia coli b3040   zinc transporter
Entamoeba histolytica EHI_103680   metal cation transporter, zinc (Zn2 )-iron (Fe2 ) permease (ZIP) family
Homo sapiens ENSG00000133195   solute carrier family 39, member 11
Loa Loa (eye worm) LOAG_12272   ZIP Zinc transporter
Mus musculus ENSMUSG00000041654   solute carrier family 39 (metal ion transporter), member 11
Mycobacterium tuberculosis Rv0318c   Probable conserved integral membrane protein
Mycobacterium ulcerans MUL_0554   transcriptional regulatory protein
Oryza sativa 4328613   Os02g0196000
Oryza sativa 4338391   Os05g0316100
Onchocerca volvulus OVOC100  
Saccharomyces cerevisiae YKL175W   Zn(2+) transporter ZRT3
Schmidtea mediterranea mk4.000242.05   Zinc transporter ZIP11
Trypanosoma cruzi TcCLB.511319.30   hypothetical protein

Essentiality

Rv0318c has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b3040 Escherichia coli non-essential goodall
CELE_F59A3.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_F59A3.4 Caenorhabditis elegans larval arrest wormbase
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv0318c (Mycobacterium tuberculosis), Probable conserved integral membrane protein
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