Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | sphingosine-1-phosphate receptor 1 | Starlite/ChEMBL | References |
Homo sapiens | sphingosine-1-phosphate receptor 3 | Starlite/ChEMBL | References |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
EC50 (binding) | = 7.7 nM | Agonist activity at human recombinant S1P1 receptor expressed in CHO cells assessed as membrane-bound 35S-GTPgammaS incubated 30 mins prior to substrate addition measured after 1 hr by topcount scintillation counting analysis | ChEMBL. | 24345087 |
EC50 (binding) | = 590 nM | Agonist activity at human recombinant S1P3 receptor expressed in CHO cells assessed as membrane-bound 35S-GTPgammaS incubated 30 mins prior to substrate addition measured after 1 hr by topcount scintillation counting analysis | ChEMBL. | 24345087 |
Emax (binding) | > 85 % | Agonist activity at human recombinant S1P3 receptor expressed in CHO cells assessed as membrane-bound 35S-GTPgammaS at 10 uM incubated 30 mins prior to substrate addition measured after 1 hr by topcount scintillation counting analysis relative to control | ChEMBL. | 24345087 |
Emax (binding) | > 85 % | Agonist activity at human recombinant S1P1 receptor expressed in CHO cells assessed as membrane-bound 35S-GTPgammaS at 10 uM incubated 30 mins prior to substrate addition measured after 1 hr by topcount scintillation counting analysis relative to control | ChEMBL. | 24345087 |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.
1 literature reference was collected for this gene.