Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | kinase insert domain receptor | Starlite/ChEMBL | No references |
Homo sapiens | epidermal growth factor receptor | Starlite/ChEMBL | No references |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Echinococcus granulosus | epidermal growth factor receptor | 0.0181 | 0.1772 | 0.1772 |
Brugia malayi | Matrixin family protein | 0.0296 | 0.3014 | 0.455 |
Schistosoma mansoni | tyrosine kinase | 0.0096 | 0.0846 | 0.1568 |
Schistosoma mansoni | matrix metallopeptidase-7 (M10 family) | 0.0296 | 0.3014 | 1 |
Echinococcus granulosus | insulin receptor | 0.0059 | 0.0443 | 0.0443 |
Brugia malayi | Matrix metalloprotease, N-terminal domain containing protein | 0.036 | 0.3713 | 0.5787 |
Echinococcus multilocularis | epidermal growth factor receptor | 0.0181 | 0.1772 | 0.1772 |
Brugia malayi | Immunoglobulin I-set domain containing protein | 0.0184 | 0.1799 | 0.2399 |
Loa Loa (eye worm) | hypothetical protein | 0.0296 | 0.3014 | 0.4356 |
Schistosoma mansoni | tyrosine kinase | 0.0181 | 0.1772 | 0.5168 |
Onchocerca volvulus | 0.0296 | 0.3014 | 0.2638 | |
Mycobacterium leprae | PROBABLE HYDROLASE | 0.036 | 0.3713 | 0.5 |
Onchocerca volvulus | 0.0284 | 0.2887 | 0.2399 | |
Echinococcus multilocularis | 0.0056 | 0.0412 | 0.0412 | |
Schistosoma mansoni | matrix metallopeptidase-9 (M10 family) | 0.0136 | 0.1281 | 0.3262 |
Onchocerca volvulus | Tyrosine kinase homolog | 0.0172 | 0.1668 | 0.01 |
Onchocerca volvulus | Matrilysin homolog | 0.0296 | 0.3014 | 0.2638 |
Onchocerca volvulus | Matrix metalloproteinase homolog | 0.0656 | 0.692 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.036 | 0.3713 | 0.5366 |
Brugia malayi | Matrixin family protein | 0.058 | 0.6094 | 1 |
Loa Loa (eye worm) | matrixin family protein | 0.0656 | 0.692 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.0296 | 0.3014 | 0.4356 |
Brugia malayi | Furin-like cysteine rich region family protein | 0.0181 | 0.1772 | 0.2352 |
Schistosoma mansoni | tyrosine kinase | 0.0096 | 0.0846 | 0.1568 |
Loa Loa (eye worm) | matrix metalloproteinase | 0.0296 | 0.3014 | 0.4356 |
Mycobacterium ulcerans | hydrolase | 0.036 | 0.3713 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0296 | 0.3014 | 0.4356 |
Echinococcus multilocularis | insulin receptor | 0.0059 | 0.0443 | 0.0443 |
Schistosoma mansoni | hypothetical protein | 0.0284 | 0.2887 | 0.9508 |
Mycobacterium tuberculosis | Probable peptidoglycan hydrolase | 0.036 | 0.3713 | 0.5 |
Schistosoma mansoni | tyrosine kinase | 0.0098 | 0.0868 | 0.1654 |
Brugia malayi | Matrixin family protein | 0.0296 | 0.3014 | 0.455 |
Echinococcus multilocularis | insulin growth factor 1 receptor beta | 0.0059 | 0.0443 | 0.0443 |
Echinococcus multilocularis | epidermal growth factor receptor | 0.0098 | 0.0868 | 0.0868 |
Loa Loa (eye worm) | matrixin family protein | 0.058 | 0.6094 | 0.8807 |
Loa Loa (eye worm) | TK/INSR protein kinase | 0.0059 | 0.0443 | 0.0639 |
Brugia malayi | Hemopexin family protein | 0.0284 | 0.2887 | 0.4326 |
Echinococcus granulosus | epidermal growth factor receptor | 0.0098 | 0.0868 | 0.0868 |
Onchocerca volvulus | Matrilysin homolog | 0.0656 | 0.692 | 1 |
Echinococcus granulosus | melanoma receptor tyrosine protein kinase | 0.0098 | 0.0868 | 0.0868 |
Schistosoma mansoni | tyrosine kinase | 0.0096 | 0.0846 | 0.1568 |
Brugia malayi | Matrixin family protein | 0.0296 | 0.3014 | 0.455 |
Brugia malayi | Matrixin family protein | 0.0296 | 0.3014 | 0.455 |
Loa Loa (eye worm) | TK/KIN16 protein kinase | 0.0184 | 0.1799 | 0.2599 |
Echinococcus granulosus | insulin growth factor 1 receptor beta | 0.0059 | 0.0443 | 0.0443 |
Schistosoma mansoni | tyrosine kinase | 0.0098 | 0.0868 | 0.1654 |
Echinococcus multilocularis | matrix metallopeptidase 7 (M10 family) | 0.094 | 1 | 1 |
Loa Loa (eye worm) | TK/EGFR protein kinase | 0.0181 | 0.1772 | 0.256 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 2 nM | BindingDB_Patents: Tyrosine Kinase Assay. EGFR1 (HER-1) enzyme assay was carried out by using Tyrosine Kinase Assay Kit Green. The entire assay was conducted in accordance with EGFR PTK inhibitor cellular activity test S.O.P.A typical kinase inhibition reaction requires a protein tyrosine kinase, a suitable buffer solution system, a peptide substrate and ATP. In the present EGFR tyrosine kinase assay, a buffer solution system containing 20 mM HEPES (pH 7.4), 5 mM MgCl2 and 2 mM MnCl2; 50 uM Na3VO4; 50 ng/10 ul EGFR (Proquinase); 100 uM ATP; and 10 ng/ml poly(Glu, Tyr) (4:1, Sigma) as a substrate were used.First, 10 ul of EGFR was added to each well of a 96-well plate, 10 ul of the test compounds diluted as described in Test Example 2 (Examples 1 to 173) was added to each well, and the plate was incubated at room temperature for 10 min. The compounds of Test Example 1 were used as control compounds at a concentration of 0.0001 to 10 uM. | ChEMBL. | No reference |
IC50 (binding) | = 2 nM | BindingDB_Patents: Tyrosine Kinase Assay. EGFR1 (HER-1) enzyme assay was carried out by using Tyrosine Kinase Assay Kit Green. The entire assay was conducted in accordance with EGFR PTK inhibitor cellular activity test S.O.P.A typical kinase inhibition reaction requires a protein tyrosine kinase, a suitable buffer solution system, a peptide substrate and ATP. In the present EGFR tyrosine kinase assay, a buffer solution system containing 20 mM HEPES (pH 7.4), 5 mM MgCl2 and 2 mM MnCl2; 50 uM Na3VO4; 50 ng/10 ul EGFR (Proquinase); 100 uM ATP; and 10 ng/ml poly(Glu, Tyr) (4:1, Sigma) as a substrate were used.First, 10 ul of EGFR was added to each well of a 96-well plate, 10 ul of the test compounds diluted as described in Test Example 2 (Examples 1 to 173) was added to each well, and the plate was incubated at room temperature for 10 min. The compounds of Test Example 1 were used as control compounds at a concentration of 0.0001 to 10 uM. | ChEMBL. | No reference |
IC50 (binding) | = 96 nM | BindingDB_Patents: Tyrosine Kinase Assay. VEGFR2 (KDR, Proquinase) enzyme assay (auto-phosphorylation assay) was carried out by using Tyrosine Kinase Assay Kit Green (Panvera). The entire assay was conducted in accordance with VEGFR PTK inhibitor cellular activity test S.O.P.A typical kinase inhibition reaction requires a protein tyrosine kinase, a suitable buffer solution system, a peptide substrate and ATP. In the present VEGFR tyrosine kinase assay, a buffer solution system containing 20 mM HEPES (pH 7.4), 5 mM MgCl2 and 2 mM MnCl2; 50 uM Na3VO4; 200 ng/10 ul VEGFR (Proquinase); 5 uM ATP; and 10 ng/ml poly(Glu, Tyr) (4:1, Sigma) as a substrate were used.First, 10 ul of VEGFR was added to each well of a 96-well plate, 10 ul of the test compounds diluted as described in Test Example 2 (Examples 1 to 173) was added to each well, and the plate was incubated at room temperature for 10 min. The compounds of Test Example 1 were used as control compounds at a concentration of 0.0001 to 10 uM. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.