Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | cytidine deaminase | Starlite/ChEMBL | No references |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Onchocerca volvulus | 0.0065 | 0.5 | 0.5 | |
Trichomonas vaginalis | cytidine deaminase, putative | 0.0065 | 0.5 | 0.5 |
Trypanosoma cruzi | cytidine deaminase-like protein, putative | 0.0065 | 0.5 | 0.5 |
Giardia lamblia | Cytidine deaminase | 0.0065 | 0.5 | 0.5 |
Leishmania major | cytidine deaminase-like protein | 0.0065 | 0.5 | 0.5 |
Echinococcus multilocularis | cytidine deaminase | 0.0065 | 0.5 | 0.5 |
Trichomonas vaginalis | cytidine deaminase, putative | 0.0065 | 0.5 | 0.5 |
Trypanosoma cruzi | cytidine deaminase-like protein | 0.0065 | 0.5 | 0.5 |
Trypanosoma brucei | cytidine deaminase | 0.0065 | 0.5 | 0.5 |
Entamoeba histolytica | cytidine deaminase, putative | 0.0065 | 0.5 | 0.5 |
Echinococcus granulosus | cytidine deaminase | 0.0065 | 0.5 | 0.5 |
Onchocerca volvulus | 0.0065 | 0.5 | 0.5 | |
Mycobacterium leprae | PROBABLE CYTIDINE DEAMINASE CDD (CYTIDINE AMINOHYDROLASE) (CYTIDINE NUCLEOSIDE DEAMINASE) | 0.0065 | 0.5 | 0.5 |
Toxoplasma gondii | cytidine and deoxycytidylate deaminase zinc-binding region domain-containing protein | 0.0065 | 0.5 | 0.5 |
Mycobacterium ulcerans | cytidine deaminase | 0.0065 | 0.5 | 0.5 |
Mycobacterium tuberculosis | Probable cytidine deaminase Cdd (cytidine aminohydrolase) (cytidine nucleoside deaminase) | 0.0065 | 0.5 | 0.5 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 400 nM | BindingDB_Patents: Enzyme Assay. The procedure to determine CDA enzymatic activity is based on published methodologies (for example, Cacciamani, T. et al., Arch. Biochem. Biophys. 1991, 290, 285-92; Cohen R. et al., J. Biol. Chem., 1971, 246, 7566-8; Vincenzetti S. et al., Protein Expr. Purif. 1996, 8, 247-53). The assay follows the change in absorbance at 286 nm of the CDA-catalyzed deamination of cytidine to form uridine. The reaction is carried out in potassium phosphate buffer (pH 7.4, 20 mM, containing 1mM DTT) in a total volume of 200 µl in a 96-well plate format. The final reaction mixture contains cytidine (50 µM) and purified human recombinant CDA. Purified enzyme is diluted so as to produce an absorbance change of approximately 2 milli-absorbance units/minute. Base line measurements of absorbance change over time are made before CDA addition to insure no change of absorbance in the absence of CDA. After CDA addition, absorbance change is monitored for 20-30 minutes. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.