Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | anaplastic lymphoma receptor tyrosine kinase | Starlite/ChEMBL | No references |
Species | Potential target | Known druggable target/s | Ortholog Group |
---|---|---|---|
Loa Loa (eye worm) | TK/ALK protein kinase | Get druggable targets OG5_132231 | All targets in OG5_132231 |
Loa Loa (eye worm) | hypothetical protein | Get druggable targets OG5_132231 | All targets in OG5_132231 |
Brugia malayi | Protein kinase domain containing protein | Get druggable targets OG5_132231 | All targets in OG5_132231 |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Echinococcus multilocularis | tyrosine protein kinase | 0.0294 | 1 | 1 |
Schistosoma mansoni | hypothetical protein | 0.0034 | 0.0352 | 1 |
Echinococcus granulosus | tyrosine protein kinase | 0.0294 | 1 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.025 | 0.8357 | 0.8357 |
Loa Loa (eye worm) | TK/ALK protein kinase | 0.029 | 0.9855 | 0.9855 |
Loa Loa (eye worm) | TK/KIN16 protein kinase | 0.0025 | 0.0022 | 0.0022 |
Loa Loa (eye worm) | hypothetical protein | 0.0034 | 0.0352 | 0.0352 |
Echinococcus multilocularis | MAM | 0.0034 | 0.0352 | 0.0352 |
Loa Loa (eye worm) | hypothetical protein | 0.0034 | 0.0352 | 0.0352 |
Echinococcus granulosus | MAM | 0.0034 | 0.0352 | 0.0352 |
Schistosoma mansoni | hypothetical protein | 0.0034 | 0.0352 | 1 |
Brugia malayi | hypothetical protein | 0.0034 | 0.0352 | 0.0352 |
Brugia malayi | Immunoglobulin I-set domain containing protein | 0.0025 | 0.0022 | 0.0022 |
Loa Loa (eye worm) | hypothetical protein | 0.0034 | 0.0352 | 0.0352 |
Loa Loa (eye worm) | hypothetical protein | 0.0288 | 0.9764 | 0.9764 |
Brugia malayi | Protein kinase domain containing protein | 0.029 | 0.9858 | 0.9858 |
Loa Loa (eye worm) | TK protein kinase | 0.0294 | 1 | 1 |
Onchocerca volvulus | 0.004 | 0.0567 | 1 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 11 nM | BindingDB_Patents: Inhibition Assay. A recombinant retrovirus was created from expression plasmid FLAG-EML4-ALKv1/pMX-iresCD8 in which cDNA for EMLA-ALK fusion protein v1 was integrated, and injected into mouse lymphoid cell line BA/F3 cells. Using a magnetic bead reagent for cell separation and a purification column (anti-CD8 monoclonal antibody immobilized on magnetic beads and a MiniMACS purification column; both are products of MilteDyi Biotec Inc.), cell surface CD8-expressing cells were purified to establish EML4-ALK fusion protein v1-expressing BA/F3 cells. From the cells, EML4-ALK fusion protein v1 was purified and subjected to kinase activity evaluation. EML4-ALK fusion protein v1 was investigated for its phosphorylation activity toward a peptide substrate by using a kinase activity detection kit (HTRF KinEASE-TK; Cisbio Inc.). Test compounds were each added to a reaction solution containing the enzyme protein to give 8 final concentrations from 1000 nM to 0.3 nM, followed by addition of ATP. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.