Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | phosphodiesterase 10A | Starlite/ChEMBL | No references |
Homo sapiens | phosphodiesterase 2A, cGMP-stimulated | Starlite/ChEMBL | No references |
Species | Potential target | Known druggable target | Length | Alignment span | Identity |
---|---|---|---|---|---|
Trypanosoma brucei | cAMP-specific phosphodiesterase | phosphodiesterase 10A | 789 aa | 666 aa | 30.2 % |
Schistosoma mansoni | camp/cgmp cyclic nucleotide phosphodiesterase | phosphodiesterase 2A, cGMP-stimulated | 934 aa | 748 aa | 27.1 % |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Brugia malayi | Kinesin motor domain containing protein | 0.0189 | 0.0913 | 0.1856 |
Plasmodium vivax | kinesin-5 | 0.0189 | 0.0913 | 0.5 |
Schistosoma mansoni | kinesin eg-5 | 0.0189 | 0.0913 | 0.0255 |
Trichomonas vaginalis | cyclic nucleotide phosphodiesterase, putative | 0.0103 | 0.0294 | 1 |
Trichomonas vaginalis | calcium/calmodulin-dependent 3,5-cyclic nucleotide phosphodiesterase, putative | 0.0103 | 0.0294 | 1 |
Mycobacterium tuberculosis | Two component sensor histidine kinase DevS | 0.0087 | 0.0181 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0297 | 0.1688 | 1 |
Loa Loa (eye worm) | kinesin-like protein KLP2 | 0.0189 | 0.0913 | 0.2072 |
Entamoeba histolytica | kinesin, putative | 0.0189 | 0.0913 | 0.5 |
Schistosoma mansoni | hypothetical protein | 0.1268 | 0.8643 | 1 |
Echinococcus multilocularis | kinesin family 1 | 0.1458 | 1 | 1 |
Plasmodium falciparum | kinesin-5 | 0.0189 | 0.0913 | 0.5 |
Brugia malayi | Probable 3',5'-cyclic phosphodiesterase C32E12.2, putative | 0.0313 | 0.1801 | 1 |
Giardia lamblia | Kinesin-5 | 0.0189 | 0.0913 | 0.5 |
Trichomonas vaginalis | conserved hypothetical protein | 0.0103 | 0.0294 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.0256 | 0.1393 | 0.6988 |
Mycobacterium ulcerans | two component sensor histidine kinase DevS | 0.0087 | 0.0181 | 0.5 |
Toxoplasma gondii | kinesin motor domain-containing protein | 0.0189 | 0.0913 | 0.5 |
Trichomonas vaginalis | rod cGMP-specific 3,5-cyclic phosphodiesterase, putative | 0.0103 | 0.0294 | 1 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 86 nM | Fluorescence Polarization Assay | BINDINGDB. | No reference |
IC50 (binding) | = 6107 nM | BindingDB_Patents: Fluorescence Polarization Assay. The inhibition of PDE 2A or 10 enzyme activity was assessed using IMAP-Phosphodiesterase-cAMP fluorescence labeled substrate (Molecular Devices, Order No. R7506), IMAP TR-FRET screening express (Molecular Devices, Order No. R8160, the TR-FRET component will not be used) and PDE 2A or PDE10 protein expressed upon baculovirus infection in SF9 cells. The cells were incubated after infection for ~3 days and protein production was confirmed by Western Blot. The cells were collected by centrifugation and the pellet frozen in liquid nitrogen before it was resuspended in PBS containing 1% Triton X-100 and protease inhibitors. After 45 min incubation on ice, the cell debris was removed by centrifugation (13.000 rpm, 30 min). Since SF 9 cells do not express cAMP hydrolyzing enzymes to a high extent, no further purification of the protein was needed.All reactions were performed in 384 well plates, Perkin Elmer black optiplates and IMAP reaction buffer with 0.1% Tween20 (kit component). | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.