Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | arachidonate 5-lipoxygenase-activating protein | Starlite/ChEMBL | No references |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Trypanosoma cruzi | spermidine synthase, putative | 0.0098 | 0.0626 | 0.5 |
Loa Loa (eye worm) | NNMT/PNMT/TEMT family protein | 0.0549 | 1 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.0549 | 1 | 1 |
Echinococcus granulosus | microsomal glutathione S transferase 3 | 0.0135 | 0.1393 | 1 |
Mycobacterium tuberculosis | Probable spermidine synthase SpeE (putrescine aminopropyltransferase) (aminopropyltransferase) (SPDSY) | 0.0068 | 0 | 0.5 |
Schistosoma mansoni | membrane associated proteins in eicosanoid and glutathione metabolism family member | 0.0135 | 0.1393 | 0.5 |
Leishmania major | spermidine synthase | 0.0098 | 0.0626 | 0.5 |
Trypanosoma cruzi | spermidine synthase, putative | 0.0098 | 0.0626 | 0.5 |
Plasmodium vivax | spermidine synthase, putative | 0.0098 | 0.0626 | 0.5 |
Trypanosoma brucei | spermidine synthase | 0.0098 | 0.0626 | 0.5 |
Trypanosoma cruzi | spermidine synthase, putative | 0.0098 | 0.0626 | 0.5 |
Trypanosoma cruzi | spermidine synthase, putative | 0.0098 | 0.0626 | 0.5 |
Plasmodium falciparum | spermidine synthase | 0.0098 | 0.0626 | 0.5 |
Mycobacterium ulcerans | spermidine synthase | 0.0068 | 0 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0549 | 1 | 1 |
Toxoplasma gondii | MAPEG family protein | 0.0135 | 0.1393 | 0.5 |
Schistosoma mansoni | microsomal glutathione s-transferase | 0.0135 | 0.1393 | 0.5 |
Echinococcus multilocularis | microsomal glutathione S transferase 3 | 0.0135 | 0.1393 | 1 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
Ki (binding) | = 5 nM | BindingDB_Patents: Homogeneous Time Resolved Fluorescence Assay. The assay below is used to test the modulatory activity of compounds against FLAP. Human and mouse FLAP-encoding DNA was amplified by polymerase chain reaction and cloned into pFastBacl (Invitrogen) with a NH2-terminal 6-His tag for expression in Spodoptera frugiperda (Sf-9) cells. FLAP-containing membranes were prepared as was a FITC-labeled FLAP modulator [5-[({[2-(2-{3-[3-(tert-Butylsulfanyl)-1-(4-chlorobenzyl)-5-(quinolin-2-ylmethoxy)-1H-indol-2-yl]-2,2-dimethylpropanoyl}hydrazino)-2-oxoethyl]sulfanyl}acetyl)amino]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid]. The FLAP binding assay is performed in HTRF format (homogeneous time resolved fluorescence). FLAP-containing membranes (1 ug/well final for human) are incubated in the presence of the HTRF ligand, [5-[({[2-(2-{3-[3-(tert-butylsulfanyl)-1-(4-chlorobenzyl)-5-(quinolin-2-ylmethoxy)-1H-indol-2-yl]-2,2-dimethylpropanoyl}hydrazino)-2-oxoethyl]sulfanyl}acetyl)amino]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid]. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.