Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Echinococcus granulosus | transient receptor potential cation channel | 0.0004 | 0.5 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0004 | 0.5 | 0.5 |
Echinococcus multilocularis | transient receptor potential cation channel | 0.0004 | 0.5 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0004 | 0.5 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0004 | 0.5 | 0.5 |
Echinococcus granulosus | transient receptor potential cation channel | 0.0004 | 0.5 | 0.5 |
Echinococcus multilocularis | transient receptor potential cation channel | 0.0004 | 0.5 | 0.5 |
Schistosoma mansoni | transient receptor potential channel | 0.0004 | 0.5 | 0.5 |
Schistosoma mansoni | transient receptor potential channel | 0.0004 | 0.5 | 0.5 |
Echinococcus granulosus | transient receptor potential cation channel | 0.0004 | 0.5 | 0.5 |
Echinococcus multilocularis | transient receptor potential cation channel | 0.0004 | 0.5 | 0.5 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | > 37500 nM | BindingDB_Patents: FLIPR Assay. The functional activity of compounds at the TRPV1 receptor was determined by measurement of intracellular Ca2+ levels ([Ca2+]i) using the Fluorescence Imaging Plate Reader (FLIPR)TETRA. All compounds were tested over a 12-point one-third-log concentration range. Compound stocks, 10 mM, were prepared in DMSO, and diluted serially across a 384-well plate using a Bravo BenchCel workstation (Agilent Technologies, Santa Clara, Calif.). A stock concentration of capsaicin (10 mM) was made in DMSO, and diluted in D-PBS to a final concentration of 200 nM (4x). On the day prior to the experiment, recombinant HEK293 cells that stably express human TRPV1 were removed from tissue culture flasks and plated in growth medium into black-walled clear-bottom 384-well Biocoat poly-D-lysine assay plates (BD Biosciences, Bedford, Mass.) using a Multidrop dispenser (ThermoScientific, Waltham, Mass.). On the day of the experiment, growth medium was removed, and the no-wash FLIPR Calcium-4 dye. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.