Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | cell division cycle 7 | No references |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Giardia lamblia | Kinase, CDC7 | 0.0069 | 0.5 | 0.5 |
Trichomonas vaginalis | CMGC family protein kinase | 0.0069 | 0.5 | 0.5 |
Onchocerca volvulus | 0.0069 | 0.5 | 0.5 | |
Loa Loa (eye worm) | CDC7 protein kinase | 0.0069 | 0.5 | 0.5 |
Trichomonas vaginalis | CMGC family protein kinase | 0.0069 | 0.5 | 0.5 |
Trichomonas vaginalis | CMGC family protein kinase | 0.0069 | 0.5 | 0.5 |
Echinococcus multilocularis | CDC7 cell division cycle 7 | 0.0069 | 0.5 | 0.5 |
Onchocerca volvulus | 0.0069 | 0.5 | 0.5 | |
Schistosoma mansoni | serine/threonine protein kinase | 0.0069 | 0.5 | 0.5 |
Echinococcus granulosus | CDC7 cell division cycle 7 | 0.0069 | 0.5 | 0.5 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 2 nM | BindingDB_Patents: MSA assay. Measurement of kinase activity was carried out using an MSA assay kit (QuickScout Screening Assist Kit, Carna Biosciences, Inc.). Assay buffer (20 mM HEPES, 0.01% Triton X-100, 2 mM dithiothreitol, pH 7.5) was used to prepare a substrate mixture solution comprising 4 µM of a kinase-reaction substrate (FITC-labeled MCM2 peptide), 40 mM MgCl2, and 20 µM ATP. The enzyme supplied in the kit (human Cdc7/human ASK complex protein) was diluted in the assay buffer so as to be at 7 nM, thereby preparing an enzyme solution. The stock solution of each test compound was diluted in DMSO to prepare diluted DMSO solutions of 10 concentrations (0.00003 mM, 0.0001 mM, 0.0003 mM, 0.001 mM, 0.003 mM, 0.01 mM, 0.03 mM, 0.1 mM, 0.3 mM, 1 mM), each of which was further diluted 25 times in the assay buffer to make a drug solution (a solution containing 4% DMSO). | ChEMBL. | No reference |
IC50 (binding) | = 2 nM | BindingDB_Patents: MSA assay. Measurement of kinase activity was carried out using an MSA assay kit (QuickScout Screening Assist Kit, Carna Biosciences, Inc.). Assay buffer (20 mM HEPES, 0.01% Triton X-100, 2 mM dithiothreitol, pH 7.5) was used to prepare a substrate mixture solution comprising 4 µM of a kinase-reaction substrate (FITC-labeled MCM2 peptide), 40 mM MgCl2, and 20 µM ATP. The enzyme supplied in the kit (human Cdc7/human ASK complex protein) was diluted in the assay buffer so as to be at 7 nM, thereby preparing an enzyme solution. The stock solution of each test compound was diluted in DMSO to prepare diluted DMSO solutions of 10 concentrations (0.00003 mM, 0.0001 mM, 0.0003 mM, 0.001 mM, 0.003 mM, 0.01 mM, 0.03 mM, 0.1 mM, 0.3 mM, 1 mM), each of which was further diluted 25 times in the assay buffer to make a drug solution (a solution containing 4% DMSO). | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.