Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | transient receptor potential cation channel, subfamily V, member 3 | No references |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Echinococcus granulosus | transient receptor potential cation channel | 0.0004 | 0.5 | 0.5 |
Echinococcus multilocularis | transient receptor potential cation channel | 0.0004 | 0.5 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0004 | 0.5 | 0.5 |
Echinococcus granulosus | transient receptor potential cation channel | 0.0004 | 0.5 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0004 | 0.5 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0004 | 0.5 | 0.5 |
Echinococcus multilocularis | transient receptor potential cation channel | 0.0004 | 0.5 | 0.5 |
Schistosoma mansoni | transient receptor potential channel | 0.0004 | 0.5 | 0.5 |
Echinococcus granulosus | transient receptor potential cation channel | 0.0004 | 0.5 | 0.5 |
Schistosoma mansoni | transient receptor potential channel | 0.0004 | 0.5 | 0.5 |
Echinococcus multilocularis | transient receptor potential cation channel | 0.0004 | 0.5 | 0.5 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 170 nM | BindingDB_Patents: Calcium Flux Assay. Experiments were conducted using the FLIPRTETRA®. On the day prior to the experiment, recombinant HEK293 cells that stably express human and mouse TRPV3 were removed from tissue culture flasks and plated in growth medium at 20,000 cells/well into black-walled clear-bottom 384-well Biocoat poly-D-lysine assay plates (BD Biosciences, Bedford, Mass.) using a Multidrop® dispenser (ThermoScientific, Waltham, Mass.). On the day of the experiment, growth medium was removed, and the no-wash FLIPR® Calcium-4 dye (¿EX=470-495 nm, ¿EM=515-575 nm; Molecular Devices, Sunnyvale, Calif.) was added to each well using the Multidrop® dispenser. Cells were incubated for 90-120 minutes in the dark. Compounds were dissolved in DMSO to prepare a 10 mM stock solution. The intensity of the fluorescence was captured and digitally transferred to an interfaced PC. | ChEMBL. | No reference |
IC50 (binding) | = 170 nM | BindingDB_Patents: Calcium Flux Assay. Experiments were conducted using the FLIPRTETRA®. On the day prior to the experiment, recombinant HEK293 cells that stably express human and mouse TRPV3 were removed from tissue culture flasks and plated in growth medium at 20,000 cells/well into black-walled clear-bottom 384-well Biocoat poly-D-lysine assay plates (BD Biosciences, Bedford, Mass.) using a Multidrop® dispenser (ThermoScientific, Waltham, Mass.). On the day of the experiment, growth medium was removed, and the no-wash FLIPR® Calcium-4 dye (¿EX=470-495 nm, ¿EM=515-575 nm; Molecular Devices, Sunnyvale, Calif.) was added to each well using the Multidrop® dispenser. Cells were incubated for 90-120 minutes in the dark. Compounds were dissolved in DMSO to prepare a 10 mM stock solution. The intensity of the fluorescence was captured and digitally transferred to an interfaced PC. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.