Detailed view for Tb927.10.2570
Basic information
Trypanosoma brucei, lysosomal alpha-mannosidase precursor, putative
Source Database / ID: TriTrypDB GeneDB
pI: 6.964 |
Length (AA): 997 |
MW (Da): 111749 |
Paralog Number:
1
Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 0
Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable
Network
Pfam domains
Gene Ontology
GO:0015923 mannosidase activity
GO:0008270 zinc ion binding
GO:0004559 alpha-mannosidase activity
GO:0003824 catalytic activity
GO:0006013 mannose metabolic process
GO:0005975 carbohydrate metabolic process
Metabolic Pathways
Structural information
Modbase 3D models:
PDB Structures:
Expression
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Mid 40-60% percentile | Procyclic, Bloodstream Form. | Siegel TN |
-
Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.
Orthologs
Ortholog group members (OG5_129192)
| Species | Accession | Gene Product |
|---|---|---|
| Arabidopsis thaliana | AT5G13980 | Glycosyl hydrolase family 38 protein |
| Arabidopsis thaliana | AT3G26720 | glycosyl hydrolase family 38 protein |
| Arabidopsis thaliana | AT5G66150 | Glycosyl hydrolase family protein |
| Caenorhabditis elegans | CELE_F55D10.1 | Protein AMAN-1 |
| Dictyostelium discoideum | DDB_G0292206 | hypothetical protein |
| Drosophila melanogaster | Dmel_CG5322 | CG5322 gene product from transcript CG5322-RB |
| Drosophila melanogaster | Dmel_CG9466 | CG9466 gene product from transcript CG9466-RB |
| Drosophila melanogaster | Dmel_CG9465 | CG9465 gene product from transcript CG9465-RB |
| Drosophila melanogaster | Dmel_CG6206 | Lysosomal alpha-mannosidase |
| Drosophila melanogaster | Dmel_CG9463 | CG9463 gene product from transcript CG9463-RB |
| Drosophila melanogaster | Dmel_CG9468 | CG9468 gene product from transcript CG9468-RA |
| Echinococcus granulosus | EgrG_000704400 | lysosomal alpha mannosidase |
| Echinococcus multilocularis | EmuJ_000704400 | lysosomal alpha mannosidase |
| Homo sapiens | ENSG00000104774 | mannosidase, alpha, class 2B, member 1 |
| Loa Loa (eye worm) | LOAG_00101 | hypothetical protein |
| Mus musculus | ENSMUSG00000005142 | mannosidase 2, alpha B1 |
| Oryza sativa | 4350617 | Os11g0525600 |
| Oryza sativa | 4348080 | Os10g0140200 |
| Schistosoma japonicum | Sjp_0032110 | Lysosomal alpha-mannosidase precursor, putative |
| Schistosoma japonicum | Sjp_0032090 | ko:K01191 alpha-mannosidase [EC3.2.1.24], putative |
| Schistosoma mansoni | Smp_131580 | lysosomal alpha-mannosidase (mannosidase alpha class 2b member 1) |
| Schmidtea mediterranea | mk4.002033.03 | |
| Schmidtea mediterranea | mk4.020205.00 | |
| Trypanosoma brucei gambiense | Tbg972.10.3200 | lysosomal alpha-mannosidase precursor, putative |
| Trypanosoma brucei | Tb11.v5.0498 | lysosomal alpha-mannosidase precursor, putative |
| Trypanosoma brucei | Tb927.10.2570 | lysosomal alpha-mannosidase precursor, putative |
| Trypanosoma cruzi | TcCLB.506195.120 | lysosomal alpha-mannosidase precursor, putative |
| Trypanosoma cruzi | TcCLB.506407.10 | lysosomal alpha-mannosidase precursor, putative |
Essentiality
| Gene/Ortholog | Organism | Phenotype | Source Study |
|---|---|---|---|
| Tb927.10.2570 this record | Trypanosoma brucei | no significant loss or gain of fitness in bloodstream forms (3 days) | alsford |
| Tb927.10.2570 this record | Trypanosoma brucei | significant gain of fitness in bloodstream forms (6 days) | alsford |
| Tb927.10.2570 this record | Trypanosoma brucei | no significant loss or gain of fitness in procyclic forms | alsford |
| Tb927.10.2570 this record | Trypanosoma brucei | significant gain of fitness in differentiation of procyclic to bloodstream forms | alsford |
-
nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource -
gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84 -
alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924 -
yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database -
shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan -
blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930 -
wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170 -
keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection -
neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
Phenotypes and Validation (curated)
Annotated phenotypes:
| Affected Entity | Phenotypic quality | Occurs in | Occurs at | Evidence | Observed in | Drugs/Inhibitors |
|---|---|---|---|---|---|---|
| cell proliferation (GO:0008283) | normal (PATO:0000461) | bloodstream stage trypomastigotes (PLO:0027) | inferred from RNAi experiment (ECO:0000019) | No drug identifiers listed for this gene. | ||
| Annotator: | fernan@iib.unsam.edu.ar. | Comment: | normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). | References: | 21363968 | |
| cell proliferation (GO:0008283) | increased (PATO:0000470) | bloodstream stage trypomastigotes (PLO:0027) | inferred from RNAi experiment (ECO:0000019) | No drug identifiers listed for this gene. | ||
| Annotator: | fernan@iib.unsam.edu.ar. | Comment: | increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). | References: | 21363968 | |
| cell proliferation (GO:0008283) | normal (PATO:0000461) | procyclic (PLO:0034) | inferred from RNAi experiment (ECO:0000019) | No drug identifiers listed for this gene. | ||
| Annotator: | fernan@iib.unsam.edu.ar. | Comment: | normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . | References: | 21363968 | |
| cell proliferation (GO:0008283) | increased (PATO:0000470) | procyclic (PLO:0034) | inferred from RNAi experiment (ECO:0000019) | No drug identifiers listed for this gene. | ||
| Annotator: | fernan@iib.unsam.edu.ar. | Comment: | increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . | References: | 21363968 | |
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
In any case, if you have information about papers containing relevant validation data for this target, please contact us.
Annotated validation
Associated compounds / Druggability
Druggability index (range: 0 to 1): 0.8
Known modulators for this target
Predicted associations
By orthology with druggable targets
| Species | Known druggable target | Linked compounds | Reference |
|---|---|---|---|
| Canavalia ensiformis | Alpha-mannosidase | Compounds | References |
| Homo sapiens | mannosidase, alpha, class 2B, member 1 | Compounds | References |
By sequence similarity to non orthologous druggable targets
Obtained from network model
Assayability
Assay information
Reagent availability
Bibliographic References
1 literature reference was collected for this gene.