Detailed view for Tb927.8.6450

Basic information

TDR Targets ID: 10132
Trypanosoma brucei, inhibitor of cysteine peptidase
Source Database / ID:  TriTrypDB  GeneDB

pI: 10.1378 | Length (AA): 121 | MW (Da): 13454 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF09394   Chagasin family peptidase inhibitor I42

Gene Ontology

Mouse over links to read term descriptions.
GO:0030162   regulation of proteolysis  
GO:0005575   cellular_component  
GO:0004866   endopeptidase inhibitor activity  

Metabolic Pathways

African trypanosomiasis (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

PDB tag
  • 2CIO:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_136685)

Species Accession Gene Product
Cryptosporidium hominis Chro.60226   transmembrane protein
Cryptosporidium parvum cgd6_1910   conserved hypothetical protein
Entamoeba histolytica EHI_159600   cysteine protease inhibitor 1 (EhICP1)
Entamoeba histolytica EHI_040460   cysteine protease inhibitor 2 (EhICP2)
Leishmania braziliensis LbrM.24.1840   inhibitor of cysteine peptidase
Leishmania donovani LdBPK_241840.1   inhibitor of cysteine peptidase
Leishmania infantum LinJ.24.1840   inhibitor of cysteine peptidase
Leishmania major LmjF.24.1770   inhibitor of cysteine peptidase
Leishmania mexicana LmxM.24.1770   inhibitor of cysteine peptidase
Trypanosoma brucei gambiense Tbg972.8.6530   inhibitor of cysteine peptidase, putative,ICP, putative
Trypanosoma brucei Tb927.8.6450   inhibitor of cysteine peptidase
Trypanosoma cruzi TcCLB.506801.80   cysteine peptidase inhibitor
Trypanosoma cruzi TcCLB.511907.200   cysteine peptidase inhibitor, putative

Essentiality

Tb927.8.6450 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.6450 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.6450 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.6450 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.6450 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Leishmania major inhibitor of cysteine peptidase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0233 0.2508 0.2473
0.0236 0.2579 0.2549
0.0275 0.269 0.2673
0.0512 0.256 0.2549
0.0272 0.2645 0.2643
0.0580982 0.331185 0.32461
0.0255 0.2613 0.2613
0.0564 0.256 0.2549
0.0235 0.2594 0.2594
0.0282 0.2697 0.2692
0.0226 0.2594 0.2594
0.0277 0.2695 0.2692
0.0265 0.2592 0.2643
0.0181 0.2582 0.2582
0.024 0.2579 0.2549
0.0252 0.2519 0.2517
0.0243 0.2594 0.2594
0.0274 0.2646 0.2643
0.0266 0.2594 0.2594
0.0255 0.2512 0.2527
0.023 0.2646 0.2643
0.0580982 0.331185 0.32461
0.0569 0.256 0.2549
0.0248 0.251 0.251
0.0256 0.2579 0.2549
0.0274 0.2579 0.2549
0.0235 0.269 0.2673
0.028 0.2646 0.2643
0.0231 0.2681 0.2673
0.0536 0.256 0.2549
0.0234 0.2594 0.2594
0.0231 0.2681 0.2673
0.0261 0.2612 0.2612
0.0229 0.2519 0.2518
0.0234 0.2594 0.2594
0.0277 0.2695 0.2692
0.0242 0.2673 0.2673
0.0277 0.2695 0.2692
0.0236 0.26 0.2594
0.0229 0.26 0.2594
0.0271 0.2594 0.2594
0.023 0.2646 0.2643
0.0265 0.2649 0.2667
0.0272 0.2646 0.2643
0.0234 0.2594 0.2594
0.0255 0.2613 0.2613
0.0282 0.2641 0.2625
0.0263 0.2511 0.2511
0.0256 0.2579 0.2549
0.0251 0.2594 0.2594
0.0564 0.2578 0.2549
0.0508 0.256 0.2549
0.0256 0.2549 0.2549
0.028 0.2646 0.2643
0.023 0.2594 0.2594
0.024 0.2579 0.2549
0.0249 0.2512 0.2517
0.0265 0.2592 0.2643
0.0233 0.2508 0.2473
0.0282 0.2697 0.2692
0.0484 0.256 0.2549
0.0564 0.256 0.2549
0.0243 0.2594 0.2594
0.0255 0.2613 0.2613
0.0536 0.256 0.2549
0.0508 0.256 0.2549
0.0256 0.269 0.2673
0.0276 0.2594 0.2594
0.0277 0.2579 0.2549
0.0512 0.256 0.2549
0.0282 0.252 0.2517
0.0569 0.256 0.2549
0.0512 0.256 0.2549
0.0261 0.2579 0.2549
0.054 0.256 0.2549
0.0128 0.2549 0.2549
0.0181 0.2561 0.2549
0.0536 0.256 0.2549
0.0254 0.2519 0.2517
0.054 0.256 0.2549
0.0241 0.2579 0.2549
0.0256 0.2629 0.2643
0.0262 0.269 0.2674
0.0274 0.2646 0.2643
0.0564 0.2549 0.2549
0.0682114 0.331076 0.32461
0.0408 0.256 0.2549
0.0232 0.2546 0.2514
0.0536 0.256 0.2549
0.0243 0.2579 0.2549
0.0233 0.2514 0.2514
0.0282 0.2697 0.2692
0.0246 0.269 0.2673
0.024 0.251 0.251
0.0484 0.256 0.2549
0.0229 0.26 0.2594
0.0564 0.2578 0.2549
0.0277 0.2646 0.2643
0.0255 0.2542 0.252
0.0569 0.256 0.2549
0.024 0.269 0.2673
0.0353971 0.305248 0.303626
0.0265 0.2549 0.2643
0.0238 0.269 0.2673
0.0181 0.2582 0.2582
0.0571 0.2578 0.2549
0.027 0.258 0.2496
0.0183 0.2741 0.2735
0.0279 0.2595 0.2594
0.0227 0.2675 0.2643
0.0277 0.2579 0.2549
0.0247 0.2579 0.2549
0.023 0.2681 0.2673
0.0229 0.2594 0.2594
0.054 0.256 0.2549
0.0256 0.2594 0.2594
0.0244 0.2681 0.2673
0.0227 0.2594 0.2594
0.0234 0.2594 0.2594
0.0251 0.2594 0.2594
0.0244 0.2514 0.2514
0.0277 0.2579 0.2549

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.8.6450 (Trypanosoma brucei), inhibitor of cysteine peptidase
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