Detailed view for Tb927.11.12910

Basic information

TDR Targets ID: 10273
Trypanosoma brucei, prefoldin subunit, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 10.0112 | Length (AA): 213 | MW (Da): 23598 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02996   Prefoldin subunit

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
23 92 3ph9 (A) 62 133 30.00 0.21 0.06 0.515139 0.88

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129956)

Species Accession Gene Product
Arabidopsis thaliana AT1G26660   Prefoldin chaperone subunit family protein
Brugia malayi Bm1_25600   hypothetical protein
Dictyostelium discoideum DDB_G0285389   prefoldin alpha subunit family protein
Drosophila melanogaster Dmel_CG15266   lethal (2) 35Cc
Echinococcus granulosus EgrG_000883900   protein uxt
Entamoeba histolytica EHI_186960   prefoldin, alpha subunit
Echinococcus multilocularis EmuJ_000883900   protein uxt
Homo sapiens ENSG00000126756   ubiquitously-expressed, prefoldin-like chaperone
Leishmania braziliensis LbrM.09.0890   hypothetical protein, conserved
Leishmania donovani LdBPK_090860.1   prefoldin subunit, putative
Leishmania infantum LinJ.09.0860   hypothetical protein, conserved
Leishmania major LmjF.09.0810   hypothetical protein, conserved
Leishmania mexicana LmxM.09.0810   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_04925   hypothetical protein
Mus musculus ENSMUSG00000001134   ubiquitously expressed transcript
Neospora caninum NCLIV_021580   hypothetical protein
Oryza sativa 4343790   Os07g0595800
Onchocerca volvulus OVOC210  
Plasmodium berghei PBANKA_0939400   prefoldin, putative
Plasmodium falciparum PF3D7_1107500   prefoldin, putative
Plasmodium knowlesi PKNH_0905100   prefoldin, putative
Plasmodium vivax PVX_091050   prefoldin, putative
Plasmodium yoelii PY03787   hypothetical protein
Schistosoma japonicum Sjp_0003440   Protein UXT, putative
Schistosoma mansoni Smp_042280.2   hypothetical protein
Trypanosoma brucei gambiense Tbg972.11.14360   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.12910   prefoldin subunit, putative
Trypanosoma congolense TcIL3000.11.13300   prefoldin subunit, putative
Trypanosoma cruzi TcCLB.505171.100   prefoldin subunit, putative
Trypanosoma cruzi TcCLB.511557.60   prefoldin subunit, putative

Essentiality

Tb927.11.12910 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.52.0009 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.52.0009 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.52.0009 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.52.0009 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.11.12910 (Trypanosoma brucei), prefoldin subunit, putative
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