Detailed view for PF3D7_0806800

Basic information

TDR Targets ID: 1046
Plasmodium falciparum, V-type proton ATPase subunit a, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 7.0906 | Length (AA): 1053 | MW (Da): 122999 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 6

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01496   V-type ATPase 116kDa subunit family

Gene Ontology

Mouse over links to read term descriptions.
GO:0046961   hydrogen ion transporting ATPase activity, rotational mechanism  
GO:0046933   hydrogen ion transporting ATP synthase activity, rotational mechanism  
GO:0033179   proton-transporting V-type ATPase, V0 domain  
GO:0005624   membrane fraction  
GO:0016469   proton-transporting two-sector ATPase complex  
GO:0015078   hydrogen ion transmembrane transporter activity  
GO:0009055   electron carrier activity  
GO:0015991   ATP hydrolysis coupled proton transport  
GO:0015986   ATP synthesis coupled proton transport  

Metabolic Pathways

Oxidative phosphorylation (KEGG)
Global map (KEGG)
Phagosome (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
22 204 4uos (A) 2 184 12.00 0.03 0.02 0.357689 -1.01
39 186 3qki (A) 30 199 28.00 0.59 0.52 0.104451 1.42

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, merozoite, sporozoite, early ring, Oocyst, Ring, Sporozoite, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile early schizont, early trophozoite, late ring, late schizont, late trophozoite, Female Gametocyte. PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile gametocyte. PlasmoDB
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_126705)

Species Accession Gene Product
Arabidopsis thaliana AT4G39080   vacuolar proton ATPase A3
Arabidopsis thaliana AT2G21410   vacuolar proton ATPase A2
Arabidopsis thaliana AT2G28520   vacuolar proton ATPase A1
Babesia bovis BBOV_III008300   V-type ATPase 116kDa subunit family protein
Brugia malayi Bm1_31340   vacuolar proton pump
Brugia malayi Bm1_46445   vacuolar proton pump
Brugia malayi Bm1_44205   V-type ATPase 116kDa subunit family protein
Candida albicans CaO19.14153   V0 domain of vacuolar H+ATPase
Candida albicans CaO19.6863   V0 domain of vacuolar H+ATPase
Candida albicans CaO19.8781   vacuolar H(+)-ATPase (V-ATPase) V0 domain subunit a
Candida albicans CaO19.1190   vacuolar H(+)-ATPase (V-ATPase) V0 domain subunit a
Caenorhabditis elegans CELE_VW02B12L.1   Protein VHA-6
Caenorhabditis elegans CELE_ZK637.8   Protein UNC-32, isoform D
Caenorhabditis elegans CELE_C26H9A.1   Protein VHA-7, isoform A
Caenorhabditis elegans CELE_F35H10.4   Protein VHA-5
Cryptosporidium hominis Chro.40167   vacuolar proton translocating ATPase 116 kDa subunit a isoform 1
Cryptosporidium parvum cgd4_1470   vacuolar proton translocating ATpase with 7 transmembrane regions near C-terminus
Dictyostelium discoideum DDB_G0291858   vacuolar ATPase transmembrane subunit
Drosophila melanogaster Dmel_CG12602   Vacuolar H[+] ATPase 100kD subunit 5
Drosophila melanogaster Dmel_CG18617   Vacuolar H[+] ATPase 100kD subunit 2
Drosophila melanogaster Dmel_CG7678   Vacuolar H[+] ATPase 100kD subunit 4
Drosophila melanogaster Dmel_CG1709   Vacuolar H[+] ATPase 100kD subunit 1
Echinococcus granulosus EgrG_000227800   V type proton ATPase 116 kDa subunit a
Echinococcus granulosus EgrG_000588900   V type proton ATPase 116 kDa subunit a
Entamoeba histolytica EHI_074020   vacuolar proton ATPase subunit, putative
Entamoeba histolytica EHI_107280   vacuolar proton ATPase subunit, putative
Echinococcus multilocularis EmuJ_000588900   V type proton ATPase 116 kDa subunit a
Echinococcus multilocularis EmuJ_000227800   V type proton ATPase 116 kDa subunit a
Giardia lamblia GL50803_18470   Vacuolar proton-ATPase subunit, putative
Homo sapiens ENSG00000185344   ATPase, H+ transporting, lysosomal V0 subunit a2
Homo sapiens ENSG00000110719   T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
Homo sapiens ENSG00000105929   ATPase, H+ transporting, lysosomal V0 subunit a4
Homo sapiens ENSG00000033627   ATPase, H+ transporting, lysosomal V0 subunit a1
Leishmania braziliensis LbrM.23.1750   vacuolar proton translocating ATPase subunit A, putative
Leishmania braziliensis LbrM.32.1010   vacuolar proton-ATPase-like protein, putative
Leishmania donovani LdBPK_231910.1   V-type proton ATPase subunit a, putative
Leishmania donovani LdBPK_320970.1   vacuolar proton-ATPase-like protein, putative
Leishmania infantum LinJ.32.0970   vacuolar proton-ATPase-like protein, putative
Leishmania infantum LinJ.23.1910   vacuolar proton translocating ATPase subunit A, putative
Leishmania major LmjF.32.0920   vacuolar proton-ATPase-like protein, putative
Leishmania major LmjF.23.1510   vacuolar proton translocating ATPase subunit A, putative
Leishmania mexicana LmxM.31.0920   vacuolar proton-ATPase-like protein, putative
Leishmania mexicana LmxM.23.1510   vacuolar proton translocating ATPase subunit A, putative
Loa Loa (eye worm) LOAG_02041   hypothetical protein
Loa Loa (eye worm) LOAG_04836   vacuolar proton pump
Loa Loa (eye worm) LOAG_00210   hypothetical protein
Loa Loa (eye worm) LOAG_05596   V-type ATPase 116kDa subunit family protein
Mus musculus ENSMUSG00000001750   T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3
Mus musculus ENSMUSG00000038600   ATPase, H+ transporting, lysosomal V0 subunit A4
Mus musculus ENSMUSG00000019302   ATPase, H+ transporting, lysosomal V0 subunit A1
Mus musculus 21871   ATPase, H+ transporting, lysosomal V0 subunit A2
Neospora caninum NCLIV_032770   hypothetical protein
Neospora caninum NCLIV_042690   vacuolar proton-translocating ATPase subunit, putative
Oryza sativa 4332270   Os03g0251500
Oryza sativa 4327412   Os01g0834200
Plasmodium berghei PBANKA_1223800   V-type proton ATPase subunit a, putative
Plasmodium falciparum PF3D7_0806800   V-type proton ATPase subunit a, putative
Plasmodium knowlesi PKNH_0112000   V-type proton ATPase subunit a, putative
Plasmodium vivax PVX_088205   vacuolar proton translocating ATPase subunit A, putative
Plasmodium yoelii PY05420   vacuolar proton translocating ATPase 116 kDa subunit a isoform 1
Saccharomyces cerevisiae YOR270C   H(+)-transporting V0 sector ATPase subunit A
Saccharomyces cerevisiae YMR054W   H(+)-transporting V0 sector ATPase subunit A
Schistosoma japonicum Sjp_0300240   ko:K02154 V-type H+-transporting ATPase subunit I, putative
Schistosoma mansoni Smp_040970.1   vacuolar proton atpases
Schistosoma mansoni Smp_040970.2   vacuolar proton atpases
Schistosoma mansoni Smp_040970.3   vacuolar proton atpases
Schmidtea mediterranea mk4.002484.00   V-type proton ATPase 116 kDa subunit a
Schmidtea mediterranea mk4.000066.25   Putative vacuolar proton atpases
Schmidtea mediterranea mk4.001301.03   V-type proton ATPase 116 kDa subunit a
Schmidtea mediterranea mk4.000066.22   V-type proton ATPase 116 kDa subunit a
Schmidtea mediterranea mk4.000066.23  
Schmidtea mediterranea mk4.000066.26   Putative vacuolar proton atpases
Trypanosoma brucei gambiense Tbg972.5.1790   vacuolar proton translocating ATPase subunit A, putative
Trypanosoma brucei Tb927.5.1300   V-type proton ATPase subunit a, putative
Trypanosoma congolense TcIL3000_0_42720   vacuolar proton translocating ATPase subunit A, putative
Trypanosoma congolense TcIL3000_5_1320   V-type proton ATPase subunit a, putative
Trypanosoma cruzi TcCLB.509601.70   V-type proton ATPase subunit a, putative
Trypanosoma cruzi TcCLB.509767.70   vacuolar proton translocating ATPase subunit A, putative
Trypanosoma cruzi TcCLB.511727.90   vacuolar proton-ATPase-like protein, putative
Toxoplasma gondii TGME49_232830   vacuolar proton translocating ATPase subunit A, putative
Toxoplasma gondii TGME49_290720   vacuolar proton translocating ATPase subunit, putative
Theileria parva TP04_0731   vacuolar ATP synthase subunit A, putative
Trichomonas vaginalis TVAG_498660   vacuolar proton ATPase, putative
Trichomonas vaginalis TVAG_075320   vacuolar proton ATPase, putative
Trichomonas vaginalis TVAG_226900   vacuolar proton ATPase subunit A4, putative

Essentiality

PF3D7_0806800 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.1300 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.1300 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.1300 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.5.1300 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_ZK637.8 Caenorhabditis elegans embryonic arrest wormbase
CELE_ZK637.8 Caenorhabditis elegans embryonic lethal wormbase
CELE_ZK637.8 Caenorhabditis elegans larval lethal wormbase
CELE_ZK637.8 Caenorhabditis elegans slow growth wormbase
CELE_ZK637.8 Caenorhabditis elegans sterile wormbase
CELE_F35H10.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_F35H10.4 Caenorhabditis elegans larval arrest wormbase
CELE_F35H10.4 Caenorhabditis elegans larval lethal wormbase
CELE_F35H10.4 Caenorhabditis elegans slow growth wormbase
CELE_VW02B12L.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_VW02B12L.1 Caenorhabditis elegans larval arrest wormbase
CELE_VW02B12L.1 Caenorhabditis elegans larval lethal wormbase
CELE_VW02B12L.1 Caenorhabditis elegans slow growth wormbase
TGME49_232830 Toxoplasma gondii Probably essential sidik
TGME49_290720 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.4

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Gallus gallus Vacuolar H(+)-transporting ATPase 116 kDa subunit, a1 isoform Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_0806800 (Plasmodium falciparum), V-type proton ATPase subunit a, putative
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