Detailed view for Tb927.11.360

Basic information

TDR Targets ID: 10461
Trypanosoma brucei, ATP-binding protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.4068 | Length (AA): 599 | MW (Da): 66678 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01902   Diphthamide synthase

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 250 3rjz (A) 5 227 30.00 0 1 0.510762 0.43
6 224 2d13 (A) 10 200 34.00 0 1 0.503509 0.54
131 239 1o50 (A) 2 113 30.00 0.21 0.28 0.23087 0.91
426 541 1jd1 (A) 10 126 8.00 0.00012 0 0.143656 0.68

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127536)

Species Accession Gene Product
Arabidopsis thaliana AT3G04480   endoribonuclease
Babesia bovis BBOV_III005100   MJ0570-related uncharacterized domain containing protein
Brugia malayi Bm1_48450   MGC83562 protein
Candida albicans CaO19.8458   similar to S. cerevisiae YLR143W
Candida albicans CaO19.839   K-dependent carboxylation domain
Caenorhabditis elegans CELE_E01A2.5   Protein E01A2.5
Cryptosporidium hominis Chro.40077   hypothetical protein
Cryptosporidium parvum cgd4_610   MJ050-like PP-loop ATpase
Dictyostelium discoideum DDB_G0278373   DUF71, ATP-binding region-containing protein
Drosophila melanogaster Dmel_CG1578   CG1578 gene product from transcript CG1578-RB
Echinococcus granulosus EgrG_000449200   meiotically up regulated gene 71 protein
Entamoeba histolytica EHI_174900   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000449200   meiotically up regulated gene 71 protein
Homo sapiens ENSG00000134146   diphthamine biosynthesis 6
Leishmania braziliensis LbrM.25.0300   hypothetical protein, conserved
Leishmania donovani LdBPK_250300.1   MJ0570-related uncharacterized domain containing protein, putative
Leishmania infantum LinJ.25.0300   hypothetical protein, conserved
Leishmania major LmjF.25.0300   hypothetical protein, conserved
Leishmania mexicana LmxM.25.0300   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_01813   ATP-binding domain-containing protein 4
Mus musculus ENSMUSG00000057147   diphthamine biosynthesis 6
Neospora caninum NCLIV_022060   hypothetical protein, conserved
Oryza sativa 9270287   Os02g0810450
Plasmodium berghei PBANKA_1437400   diphthine--ammonia ligase, putative
Plasmodium falciparum PF3D7_1222500   diphthine--ammonia ligase, putative
Plasmodium knowlesi PKNH_1441600   diphthine--ammonia ligase, putative
Plasmodium vivax PVX_123755   hypothetical protein, conserved
Saccharomyces cerevisiae YLR143W   Dph6p
Schistosoma mansoni Smp_184390   hypothetical protein
Schistosoma mansoni Smp_186240   hypothetical protein
Schmidtea mediterranea mk4.000035.09   DiphthineALQ-ammonia ligase
Trypanosoma brucei gambiense Tbg972.11.240   ATP-binding protein, putative
Trypanosoma brucei Tb927.11.360   ATP-binding protein, putative
Trypanosoma congolense TcIL3000.11.300   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.503917.14   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.507093.66   hypothetical protein, conserved
Toxoplasma gondii TGME49_202850   ATP-binding domain-containing protein
Theileria parva TP02_0444   hypothetical protein, conserved

Essentiality

Tb927.11.360 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.03.0780 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.03.0780 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.03.0780 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.03.0780 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_202850 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.11.360 (Trypanosoma brucei), ATP-binding protein, putative
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