Detailed view for Tb927.4.1080

Basic information

TDR Targets ID: 11245
Trypanosoma brucei, V-type ATPase, A subunit, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.6158 | Length (AA): 610 | MW (Da): 67750 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00006   ATP synthase alpha/beta family, nucleotide-binding domain
PF02874   ATP synthase alpha/beta family, beta-barrel domain
PF16886   ATPsynthase alpha/beta subunit N-term extension

Gene Ontology

Mouse over links to read term descriptions.
GO:1902600   GO:proton transmembrane transport  

GO:0046961   hydrogen ion transporting ATPase activity, rotational mechanism  
GO:0033180   proton-transporting V-type ATPase, V1 domain  
GO:0016469   proton-transporting two-sector ATPase complex  
GO:0005524   ATP binding  
GO:0046034   ATP metabolic process  
GO:0015992   proton transport  
GO:0015991   ATP hydrolysis coupled proton transport  
GO:0015986   ATP synthesis coupled proton transport  

Metabolic Pathways

Oxidative phosphorylation (KEGG)
Global map (KEGG)
Phagosome (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127237)

Species Accession Gene Product
Arabidopsis thaliana AT1G78900   V-type proton ATPase catalytic subunit A
Babesia bovis BBOV_III008150   vacuolar ATP synthase catalytic subunit A
Brugia malayi Bm1_50960   vacuolar ATP synthase catalytic subunit A, osteoclast isoform
Candida albicans CaO19.9249   likely vacuolar ATPase V1 complex subunit A similar to S. cerevisiae TFP1 (YDL185W)
Caenorhabditis elegans CELE_Y49A3A.2   Protein VHA-13
Cryptosporidium hominis Chro.70559   vacuolar ATP synthase catalytic subunit a
Cryptosporidium parvum cgd7_5000   vacuolar ATP synthase subunit A
Chlamydia trachomatis CT_308   V-type ATP synthase subunit A
Dictyostelium discoideum DDB_G0287127   vacuolar H+-ATPase A subunit
Drosophila melanogaster Dmel_CG5075   Vacuolar H[+] ATPase 68kD subunit 3
Drosophila melanogaster Dmel_CG12403   Vacuolar H[+] ATPase 68kD subunit 1
Drosophila melanogaster Dmel_CG3762   Vacuolar H[+] ATPase 68 kDa subunit 2
Echinococcus granulosus EgrG_000183100   vacuolar H ATPase v1 sector subunit A
Entamoeba histolytica EHI_043010   V-type ATPase, A subunit, putative
Echinococcus multilocularis EmuJ_000183100   vacuolar H+ ATPase v1 sector subunit A
Giardia lamblia GL50803_7532   Vacuolar ATP synthase catalytic subunit A
Homo sapiens ENSG00000114573   ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
Leishmania braziliensis LbrM.20.3260   vacuolar ATP synthase catalytic subunit A, putative
Leishmania donovani LdBPK_343460.1   vacuolar ATP synthase catalytic subunit A, putative
Leishmania infantum LinJ.34.3460   vacuolar ATP synthase catalytic subunit A, putative
Leishmania major LmjF.34.3670   vacuolar ATP synthase catalytic subunit A, putative
Leishmania mexicana LmxM.33.3670   vacuolar ATP synthase catalytic subunit A, putative
Loa Loa (eye worm) LOAG_08611   hypothetical protein
Loa Loa (eye worm) LOAG_01530   vacuolar H ATPase family member
Mus musculus ENSMUSG00000052459   ATPase, H+ transporting, lysosomal V1 subunit A
Neospora caninum NCLIV_029990   vacuolar ATP synthase catalytic subunit A, putative
Oryza sativa 4341751   Os06g0662000
Oryza sativa 4328470   Os02g0175400
Onchocerca volvulus OVOC4417  
Plasmodium berghei PBANKA_1410400   V-type proton ATPase catalytic subunit A, putative
Plasmodium falciparum PF3D7_1311900   V-type proton ATPase catalytic subunit A
Plasmodium knowlesi PKNH_1412600   V-type proton ATPase catalytic subunit A, putative
Plasmodium vivax PVX_122430   vacuolar ATP synthase catalytic subunit A, putative
Plasmodium yoelii PY01556   V-type ATPase, A subunit
Saccharomyces cerevisiae YDL185W   H(+)-transporting V1 sector ATPase subunit A
Schistosoma japonicum Sjp_0213820   ko:K02145 V-type H+-transporting ATPase subunit A, putative
Schistosoma mansoni Smp_147050   ATP synthase alpha subunit vacuolar
Schmidtea mediterranea mk4.042236.00  
Schmidtea mediterranea mk4.012972.00  
Trypanosoma brucei gambiense Tbg972.4.910   V-type ATPase, A subunit, putative
Trypanosoma brucei Tb927.4.1080   V-type ATPase, A subunit, putative
Trypanosoma congolense TcIL3000_4_700   V-type ATPase, A subunit, putative
Trypanosoma cruzi TcCLB.506405.120   V-type ATPase, A subunit, putative
Trypanosoma cruzi TcCLB.503929.10   V-type ATPase, A subunit, putative
Toxoplasma gondii TGME49_256970   vacuolar ATP synthase subunit A, putative
Treponema pallidum TP0529   V-type ATP synthase subunit A
Treponema pallidum TP0426   V-type ATP synthase subunit A
Theileria parva TP04_0714   vacuolar ATP synthase catalytic subunit A, putative
Trichomonas vaginalis TVAG_420260   ATP synthase beta subunit, putative

Essentiality

Tb927.4.1080 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.1080 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.4.1080 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.4.1080 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.4.1080 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y49A3A.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y49A3A.2 Caenorhabditis elegans larval arrest wormbase
CELE_Y49A3A.2 Caenorhabditis elegans slow growth wormbase
CELE_Y49A3A.2 Caenorhabditis elegans sterile wormbase
PBANKA_1410400 Plasmodium berghei Essential plasmo
TGME49_256970 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.0092 0.3006 0.3074

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

38 literature references were collected for this gene.

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Gene identifier Tb927.4.1080 (Trypanosoma brucei), V-type ATPase, A subunit, putative
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