Detailed view for Tb927.5.3450

Basic information

TDR Targets ID: 11253
Trypanosoma brucei, eukaryotic translation initiation factor eIF2A, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.9903 | Length (AA): 527 | MW (Da): 57992 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF08662   Eukaryotic translation initiation factor eIF2A

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128129)

Species Accession Gene Product
Arabidopsis thaliana AT1G73180   eukaryotic translation initiation factor eIF2A-like protein
Babesia bovis BBOV_IV009900   conserved hypothetical protein
Brugia malayi Bm1_53410   Hypothetical 71.3 kDa protein in SCM4-MUP1 intergenic region
Brugia malayi Bm1_02635   LOC398445 protein
Candida albicans CaO19.2930   signaling protein,mating, glycosylation
Candida albicans CaO19.10447   signaling protein,mating, glycosylation
Caenorhabditis elegans CELE_E04D5.1   Protein E04D5.1, isoform B
Cryptosporidium hominis Chro.70110   hypothetical protein
Cryptosporidium parvum cgd7_880   eIF-3A like translation initiation factor that has a WD40 repeat-containing beta propeller
Dictyostelium discoideum DDB_G0284267   eukaryotic translation initiation factor 2A
Drosophila melanogaster Dmel_CG7414   CG7414 gene product from transcript CG7414-RC
Echinococcus granulosus EgrG_000525500   eukaryotic translation initiation factor 2A
Entamoeba histolytica EHI_200070   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000525500   eukaryotic translation initiation factor 2A
Homo sapiens 83939   eukaryotic translation initiation factor 2A, 65kDa
Leishmania braziliensis LbrM.16.0680   hypothetical protein, conserved
Leishmania donovani LdBPK_160690.1   eukaryotic translation initiation factor eIF2A, putative
Leishmania infantum LinJ.16.0690   hypothetical protein, conserved
Leishmania major LmjF.16.0690   hypothetical protein, conserved
Leishmania mexicana LmxM.16.0690   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_04420   hypothetical protein
Loa Loa (eye worm) LOAG_04419   hypothetical protein
Mus musculus ENSMUSG00000027810   eukaryotic translation initiation factor 2A
Neospora caninum NCLIV_027770   hypothetical protein
Oryza sativa 4329922   Os02g0606100
Onchocerca volvulus OVOC1836   Eukaryotic translation initiation factor 2A homolog
Plasmodium berghei PBANKA_0610800   eukaryotic initiation factor 2a, putative
Plasmodium falciparum PF3D7_1438000   eukaryotic translation initiation factor eIF2A, putative
Plasmodium knowlesi PKNH_0424700   eukaryotic initiation factor 2a, putative
Plasmodium knowlesi PKNH_0838000   eukaryotic translation initiation factor eIF2A, putative
Plasmodium vivax PVX_084645   hypothetical protein, conserved
Plasmodium yoelii PY02477   unknown protein
Saccharomyces cerevisiae YGR054W   hypothetical protein
Schistosoma japonicum Sjp_0204810   Eukaryotic translation initiation factor 2A, putative
Schistosoma mansoni Smp_175840   nuclease-related
Schmidtea mediterranea mk4.007504.00  
Trypanosoma brucei gambiense Tbg972.5.4830   hypothetical protein, conserved
Trypanosoma brucei Tb927.5.3450   eukaryotic translation initiation factor eIF2A, putative
Trypanosoma congolense TcIL3000_5_3910   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.511647.60   eukaryotic translation initiation factor eIF2A, putative
Trypanosoma cruzi TcCLB.503529.20   eukaryotic translation initiation factor eIF2A, putative
Toxoplasma gondii TGME49_258740   eukaryotic initiation factor-2A, putative
Theileria parva TP01_0707   hypothetical protein
Trichomonas vaginalis TVAG_139050   conserved hypothetical protein

Essentiality

Tb927.5.3450 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.3450 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.3450 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.3450 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.3450 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_E04D5.1 Caenorhabditis elegans embryonic lethal wormbase
TGME49_258740 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.5.3450 (Trypanosoma brucei), eukaryotic translation initiation factor eIF2A, putative
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