Detailed view for Tb927.1.1380

Basic information

TDR Targets ID: 11330
Trypanosoma brucei, serine/threonine protein phosphatase 2A regulatory subunit, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.552 | Length (AA): 573 | MW (Da): 62615 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0008287   protein serine/threonine phosphatase complex  
GO:0005488   binding  
GO:0004722   protein serine/threonine phosphatase activity  
GO:0006470   protein amino acid dephosphorylation  

Metabolic Pathways

mRNA surveillance pathway (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 573 2qmr (C) 44 690 16.00 0 1 1.11965 0.28
7 41 5aei (A) 222 256 14.00 0.13 0.02 0.339182 -1.52
8 572 1b3u (A) 12 580 34.00 0 1 1.42344 -0.6
310 434 3vwa (A) 415 541 15.00 0 0.69 0.50955 -1.15

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127382)

Species Accession Gene Product
Arabidopsis thaliana AT3G25800   protein phosphatase 2A subunit A2
Arabidopsis thaliana AT1G25490   serine/threonine-protein phosphatase 2A regulatory subunit A alpha isoform
Arabidopsis thaliana AT1G13320   protein phosphatase 2A subunit A3
Babesia bovis BBOV_II001110   HEAT repeat containing protein
Brugia malayi Bm1_14145   protein phosphatase PP2A regulatory subunit
Candida albicans CaO19.14102   protein phosphatase 2A regulatory subunit A
Candida albicans CaO19.6810   protein phosphatase 2A regulatory subunit A
Caenorhabditis elegans CELE_F48E8.5   Protein PAA-1
Dictyostelium discoideum DDB_G0283601   protein phosphatase 2A scaffold subunit
Drosophila melanogaster Dmel_CG17291   Protein phosphatase 2A at 29B
Echinococcus granulosus EgrG_000598100   Serine:threonine protein phosphatase 2A 65 kDa
Echinococcus granulosus EgrG_001152800   Serine:threonine protein phosphatase 2A 65 kDa
Entamoeba histolytica EHI_124870   HEAT repeat domain containing protein
Echinococcus multilocularis EmuJ_001152800   Serine:threonine protein phosphatase 2A 65 kDa
Echinococcus multilocularis EmuJ_000598100   Serine:threonine protein phosphatase 2A 65 kDa
Giardia lamblia GL50803_7439   Ser/Thr phosphatase 2A, 65kDa reg sub A
Homo sapiens 5518   protein phosphatase 2, regulatory subunit A, alpha
Homo sapiens ENSG00000137713   protein phosphatase 2, regulatory subunit A, beta
Leishmania braziliensis LbrM.20.4890   serine/threonine protein phosphatase 2A regulatory subunit, putative
Leishmania donovani LdBPK_200720.1   serine/threonine protein phosphatase 2A regulatory subunit, putative
Leishmania infantum LinJ.20.0720   serine/threonine protein phosphatase 2A regulatory subunit, putative
Leishmania major LmjF.20.0660   serine/threonine protein phosphatase 2A regulatory subunit, putative
Leishmania mexicana LmxM.20.0660   serine/threonine protein phosphatase 2A regulatory subunit, putative
Loa Loa (eye worm) LOAG_00611   protein phosphatase PP2A regulatory subunit
Mus musculus ENSMUSG00000007564   protein phosphatase 2, regulatory subunit A, alpha
Mus musculus ENSMUSG00000032058   protein phosphatase 2, regulatory subunit A, beta
Neospora caninum NCLIV_058340   hypothetical protein
Oryza sativa 4346507   Os09g0249700
Plasmodium berghei PBANKA_1418200   protein phosphatase PP2A regulatory subunit A, putative
Plasmodium falciparum PF3D7_1319700   protein phosphatase PP2A regulatory subunit A, putative
Plasmodium knowlesi PKNH_1420500   protein phosphatase PP2A regulatory subunit A, putative
Plasmodium vivax PVX_122800   protein phosphatase PP2A regulatory subunit A, putative
Plasmodium yoelii PY01941   similar to protein phosphatase 2
Saccharomyces cerevisiae YAL016W   protein phosphatase 2A structural subunit TPD3
Schistosoma japonicum Sjp_0112840   similar to Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, putative
Schistosoma japonicum Sjp_0101580   expressed protein
Schistosoma japonicum Sjp_0029580   ko:K03456 protein phosphatase 2 (formerly 2A), regulatory subunit A, putative
Schistosoma japonicum Sjp_0096340   similar to Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, putative
Schistosoma japonicum Sjp_0113240   IPR004018,RPEL repeat,domain-containing
Schistosoma mansoni Smp_172290   serine/threonine protein phosphatase 2a regulatory subunit A
Schistosoma mansoni Smp_153410   serine/threonine protein phosphatase 2a regulatory subunit A
Schistosoma mansoni Smp_172280   serine/threonine protein phosphatase 2a regulatory subunit A
Schmidtea mediterranea mk4.000417.05   Probable serine/threonine-protein phosphatase PP2A regulatory subunit
Schmidtea mediterranea mk4.004359.01   Probable serine/threonine-protein phosphatase PP2A regulatory subunit
Schmidtea mediterranea mk4.000440.04   Serine/threonine-protein phosphatase PP2A 65 kDa regulatory subunit
Schmidtea mediterranea mk4.028353.00  
Schmidtea mediterranea mk4.000417.04   Serine/threonine-protein phosphatase PP2A 65 kDa regulatory subunit
Trypanosoma brucei gambiense Tbg972.1.680   serine/threonine protein phosphatase 2A regulatory subunit, putative
Trypanosoma brucei Tb927.1.1380   serine/threonine protein phosphatase 2A regulatory subunit, putative
Trypanosoma brucei Tb11.v5.0390   serine/threonine protein phosphatase 2A regulatory subunit, putative
Trypanosoma congolense TcIL3000_1_830   serine/threonine protein phosphatase 2A regulatory subunit, putative
Trypanosoma congolense TcIL3000_0_17800   serine/threonine protein phosphatase 2A regulatory subunit, putative
Trypanosoma cruzi TcCLB.506479.39   serine/threonine protein phosphatase 2A regulatory subunit, putative
Trypanosoma cruzi TcCLB.511425.10   serine/threonine protein phosphatase 2A regulatory subunit, putative
Theileria parva TP04_0474   Serine/threonine protein phosphatase, putative
Trichomonas vaginalis TVAG_494010   serine/threonine protein phosphatase 2A regulatory subunit A, putative
Trichomonas vaginalis TVAG_302330   serine/threonine protein phosphatase 2A regulatory subunit A, putative
Trichomonas vaginalis TVAG_459000   serine/threonine protein phosphatase 2A regulatory subunit A, putative
Trichomonas vaginalis TVAG_122820   conserved hypothetical protein

Essentiality

Tb927.1.1380 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.1.1380 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.1.1380 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.1.1380 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.1.1380 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F48E8.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_F48E8.5 Caenorhabditis elegans larval lethal wormbase
CELE_F48E8.5 Caenorhabditis elegans sterile wormbase
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
cell morphogenesis (GO:0000902) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
regulation of cell motility (GO:0051270) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
cell cycle (GO:0007049) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
mitochondrion organization and biogenesis (GO:0007005) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
regulation of cell motility (GO:0051270) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
endocytosis (GO:0006897) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Mus musculus protein phosphatase 2, regulatory subunit A, alpha Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.1.1380 (Trypanosoma brucei), serine/threonine protein phosphatase 2A regulatory subunit, putative
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