Detailed view for Tb927.8.2540

Basic information

TDR Targets ID: 11345
Trypanosoma brucei, 3-ketoacyl-CoA thiolase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.7306 | Length (AA): 438 | MW (Da): 46245 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00108   Thiolase, N-terminal domain
PF02803   Thiolase, C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016747   transferase activity, transferring groups other than amino-acyl groups  
GO:0008150   biological_process  
GO:0003988   acetyl-CoA C-acyltransferase activity  
GO:0005739   mitochondrion  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

PDB tag
  • 4BI9:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4BIA:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128557)

Species Accession Gene Product
Brugia malayi Bm1_53275   sterol carrier protein
Caenorhabditis elegans CELE_Y57A10C.6   Protein DAF-22
Drosophila melanogaster Dmel_CG17597   CG17597 gene product from transcript CG17597-RB
Drosophila melanogaster Dmel_CG17320   Sterol carrier protein X-related thiolase
Homo sapiens ENSG00000116171   sterol carrier protein 2
Leishmania braziliensis LbrM.23.0840   3-ketoacyl-CoA thiolase, putative
Leishmania donovani LdBPK_230860.1   3-ketoacyl-CoA thiolase-like protein
Leishmania infantum LinJ.23.0860   3-ketoacyl-CoA thiolase, putative
Leishmania major LmjF.23.0690   3-ketoacyl-CoA thiolase, putative
Leishmania mexicana LmxM.23.0690   3-ketoacyl-CoA thiolase-like protein
Loa Loa (eye worm) LOAG_16226   hypothetical protein
Loa Loa (eye worm) LOAG_15893   hypothetical protein
Loa Loa (eye worm) LOAG_13682   hypothetical protein
Loa Loa (eye worm) LOAG_13329   hypothetical protein
Loa Loa (eye worm) LOAG_12152   hypothetical protein
Loa Loa (eye worm) LOAG_15100   hypothetical protein
Loa Loa (eye worm) LOAG_13959   hypothetical protein
Loa Loa (eye worm) LOAG_14544   hypothetical protein
Mus musculus ENSMUSG00000028603   sterol carrier protein 2, liver
Mycobacterium tuberculosis Rv0914c   Possible lipid carrier protein or keto acyl-CoA thiolase
Mycobacterium tuberculosis Rv2790c   Probable lipid-transfer protein Ltp1
Mycobacterium ulcerans MUL_4955   lipid-transfer protein Ltp1
Mycobacterium ulcerans MUL_2143   lipid-transfer protein
Schmidtea mediterranea mk4.002756.03   Non-specific lipid-transfer protein-like
Schmidtea mediterranea mk4.003829.02  
Schmidtea mediterranea mk4.005896.01   Non-specific lipid-transfer protein
Schmidtea mediterranea mk4.023837.01   Non-specific lipid-transfer protein-like
Schmidtea mediterranea mk4.025329.00   Non-specific lipid-transfer protein
Schmidtea mediterranea mk4.078445.00  
Schmidtea mediterranea mk4.049830.00   Non-specific lipid-transfer protein-like
Schmidtea mediterranea mk4.016650.00  
Schmidtea mediterranea mk4.007043.00   Non-specific lipid-transfer protein
Schmidtea mediterranea mk4.030657.00   Non-specific lipid-transfer protein
Schmidtea mediterranea mk4.003904.00  
Trypanosoma brucei Tb927.8.2540   3-ketoacyl-CoA thiolase, putative
Trypanosoma cruzi TcCLB.510507.20   3-ketoacyl-CoA thiolase, putative
Trypanosoma cruzi TcCLB.509463.30   3-ketoacyl-CoA thiolase, putative

Essentiality

Tb927.8.2540 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2837 Mycobacterium tuberculosis non-essential nmpdr
mtu930 Mycobacterium tuberculosis non-essential nmpdr
Tb927.8.2540 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.2540 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.2540 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.2540 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y57A10C.6 Caenorhabditis elegans larval arrest wormbase
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens sterol carrier protein 2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

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Gene identifier Tb927.8.2540 (Trypanosoma brucei), 3-ketoacyl-CoA thiolase, putative
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