Detailed view for Tb927.9.5210

Basic information

TDR Targets ID: 11373
Trypanosoma brucei, glutaminyl-tRNA synthetase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.7107 | Length (AA): 577 | MW (Da): 67278 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00749   tRNA synthetases class I (E and Q), catalytic domain
PF03950   tRNA synthetases class I (E and Q), anti-codon binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0005524   ATP binding  
GO:0004819   glutamine-tRNA ligase activity  
GO:0004812   aminoacyl-tRNA ligase activity  
GO:0000166   nucleotide binding  
GO:0043039   tRNA aminoacylation  
GO:0006425   glutaminyl-tRNA aminoacylation  
GO:0006418   tRNA aminoacylation for protein translation  
GO:0006412   translation  

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127471)

Species Accession Gene Product
Arabidopsis thaliana AT1G25350   putative tRNA-glutamine ligase
Babesia bovis BBOV_III006400   glutaminyl-tRNA synthetase family protein
Brugia malayi Bm1_12965   glutaminyl-tRNA synthetase
Candida albicans CaO19_7064   hypothetical protein
Candida albicans CaO19.7064   glutaminyl-tRNA synthetase
Caenorhabditis elegans CELE_Y41E3.4   Protein QARS-1, isoform D
Cryptosporidium hominis Chro.10244   glutaminyl-tRNA synthetase
Cryptosporidium parvum cgd1_2130   glutaminyl-tRNA synthetase, of predicted bacterial origin
Dictyostelium discoideum DDB_G0289481   glutamine-tRNA ligase
Drosophila melanogaster Dmel_CG10506   Glutaminyl-tRNA synthetase
Escherichia coli b0680   glutamyl-tRNA synthetase
Echinococcus granulosus EgrG_000248300   glutaminyl tRNA synthetase
Entamoeba histolytica EHI_062700   glutaminyl-tRNA synthetase, putative
Echinococcus multilocularis EmuJ_000248300   glutaminyl tRNA synthetase
Giardia lamblia GL50803_9348   Glutaminyl-tRNA synthetase
Homo sapiens ENSG00000172053   glutaminyl-tRNA synthetase
Leishmania braziliensis LbrM.15.1430   glutaminyl-tRNA synthetase, putative
Leishmania donovani LdBPK_151490.1   glutaminyl-tRNA synthetase, putative
Leishmania infantum LinJ.15.1490   glutaminyl-tRNA synthetase, putative
Leishmania major LmjF.15.1440   glutaminyl-tRNA synthetase, putative
Leishmania mexicana LmxM.15.1440   glutaminyl-tRNA synthetase, putative
Loa Loa (eye worm) LOAG_11368   glutaminyl-tRNA synthetase
Loa Loa (eye worm) LOAG_05553   hypothetical protein
Mus musculus ENSMUSG00000032604   glutaminyl-tRNA synthetase
Neospora caninum NCLIV_062230   Glutaminyl-tRNA synthetase (EC 6.1.1.18), related
Neospora caninum NCLIV_062220   Glutaminyl-tRNA synthetase (EC 6.1.1.18), related
Oryza sativa 4337996   Os05g0182800
Oryza sativa 4325653   Os01g0185200
Plasmodium berghei PBANKA_1346600   glutamine--tRNA ligase, putative
Plasmodium falciparum PF3D7_1331700   glutamine--tRNA ligase, putative
Plasmodium knowlesi PKNH_1268800   glutamine--tRNA ligase, putative
Plasmodium vivax PVX_082520   glutamine--tRNA ligase, putative
Plasmodium yoelii PY02178   glutaminyl-tRNA synthetase, putative
Saccharomyces cerevisiae YOR168W   glutamine--tRNA ligase
Schistosoma japonicum Sjp_0203800   ko:K01886 glutaminyl-tRNA synthetase [EC6.1.1.18], putative
Schistosoma mansoni Smp_148050   glutaminyl-tRNA synthetase (glutamine--tRNA ligase) (glnrs)
Schmidtea mediterranea mk4.071411.00  
Schmidtea mediterranea mk4.002657.02   Probable glutamineALQ-tRNA ligase
Schmidtea mediterranea mk4.002223.03   Probable glutamineALQ-tRNA ligase
Schmidtea mediterranea mk4.001346.07   Probable glutamineALQ-tRNA ligase
Schmidtea mediterranea mk4.002657.03   Probable glutamineALQ-tRNA ligase
Trypanosoma brucei gambiense Tbg972.9.2750   glutaminyl-tRNA synthetase, putative
Trypanosoma brucei Tb927.9.5210   glutaminyl-tRNA synthetase, putative
Trypanosoma congolense TcIL3000_9_1710   glutaminyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.508277.160   glutaminyl-tRNA synthetase, putative
Toxoplasma gondii TGME49_217460   glutaminyl-tRNA synthetase (GlnRS)
Theileria parva TP02_0322   glutaminyl-tRNA synthetase, putative
Trichomonas vaginalis TVAG_258380   glutaminyl-tRNA synthetase, putative

Essentiality

Tb927.9.5210 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.3730 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.160.3730 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.160.3730 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.160.3730 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b0680 Escherichia coli essential goodall
CELE_Y41E3.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y41E3.4 Caenorhabditis elegans larval arrest wormbase
CELE_Y41E3.4 Caenorhabditis elegans slow growth wormbase
YOR168W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1346600 Plasmodium berghei Essential plasmo
TGME49_217460 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.8

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens glutaminyl-tRNA synthetase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Tb927.9.5210 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Trypanosoma brucei ( 1 )

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.9.5210 (Trypanosoma brucei), glutaminyl-tRNA synthetase, putative
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