Detailed view for Tb927.8.1980

Basic information

TDR Targets ID: 11741
Trypanosoma brucei, Beta propeller protein 1
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.0399 | Length (AA): 477 | MW (Da): 53365 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00400   WD domain, G-beta repeat
PF09384   UTP15 C terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0003674   molecular_function  
GO:0005730   nucleolus  
GO:0005515   protein binding  
GO:0006364   rRNA processing  

Metabolic Pathways

Ribosome biogenesis in eukaryotes (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
15 319 5kwn (A) 358 675 23.00 0.00006 1 0.847313 -0.01
112 236 4aow (A) 99 227 32.00 0.0000000024 0.98 0.620955 -0.34
133 198 5fa5 (B) 141 204 42.00 0.0003 0.19 0.486265 -0.12
170 317 4jsn (D) 12 157 21.00 0.0000023 0.91 0.619173 -0.31

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128309)

Species Accession Gene Product
Arabidopsis thaliana AT2G47990   transducin family nucleolar protein with WD40 repeats
Babesia bovis BBOV_IV004010   WD domain, G-beta repeat containing protein
Brugia malayi Bm1_14340   Src-associated protein SAW
Candida albicans CaO19.11092   similar to S. cerevisiae YMR093W
Candida albicans CaO19.3609   similar to S. cerevisiae YMR093W
Caenorhabditis elegans CELE_Y23H5B.5   Protein Y23H5B.5
Cryptosporidium hominis Chro.30266   Flj12787-prov protein
Cryptosporidium parvum cgd3_2270   WD repeat protein
Dictyostelium discoideum DDB_G0283581   U3 small nucleolar ribonucleoprotein
Drosophila melanogaster Dmel_CG3071   CG3071 gene product from transcript CG3071-RA
Echinococcus granulosus EgrG_000147200   U3 small nucleolar RNA associated protein 15
Entamoeba histolytica EHI_170100   WD domain containing protein
Echinococcus multilocularis EmuJ_000147200   U3 small nucleolar RNA associated protein 15
Echinococcus multilocularis EmuJ_000136900   U3 small nucleolar RNA associated protein 15
Giardia lamblia GL50803_15026   Src-associated protein-like protein
Homo sapiens ENSG00000164338   UTP15, U3 small nucleolar ribonucleoprotein, homolog (S. cerevisiae)
Leishmania braziliensis LbrM.23.0040   beta propeller protein, putative
Leishmania donovani LdBPK_230040.1   Beta propeller protein 1
Leishmania infantum LinJ.23.0040   beta propeller protein, putative
Leishmania major LmjF.23.0030   beta propeller protein, putative
Leishmania mexicana LmxM.23.0030   beta propeller protein, putative
Loa Loa (eye worm) LOAG_00216   src-associated protein SAW
Mus musculus ENSMUSG00000041747   UTP15, U3 small nucleolar ribonucleoprotein, homolog (yeast)
Neospora caninum NCLIV_016210   WD domain, G-beta repeat-containing protein, putative
Oryza sativa 4342750   Os07g0224900
Saccharomyces cerevisiae YMR093W   Utp15p
Schistosoma japonicum Sjp_0064900   U3 small nucleolar RNA-associated protein 15 homolog, putative
Schistosoma mansoni Smp_045070   hypothetical protein
Schmidtea mediterranea mk4.000054.07  
Trypanosoma brucei gambiense Tbg972.8.1640   hypothetical protein, conserved
Trypanosoma brucei Tb927.8.1980   Beta propeller protein 1
Trypanosoma congolense TcIL3000_8_2010   Beta propeller protein 1
Trypanosoma cruzi TcCLB.509499.20   Beta propeller protein 1
Trypanosoma cruzi TcCLB.508649.10   Beta propeller protein 1
Toxoplasma gondii TGME49_239590   WD domain, G-beta repeat-containing protein
Theileria parva TP01_1027   hypothetical protein
Trichomonas vaginalis TVAG_462350   conserved hypothetical protein

Essentiality

Tb927.8.1980 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.1980 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.1980 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.1980 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.1980 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
YMR093W Saccharomyces cerevisiae inviable yeastgenome
TGME49_239590 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.8.1980 (Trypanosoma brucei), Beta propeller protein 1
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