Detailed view for Tb927.6.4520

Basic information

TDR Targets ID: 12018
Trypanosoma brucei, Tumour suppressor, Mitostatin, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.8442 | Length (AA): 436 | MW (Da): 52589 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13868   Trichohyalin-plectin-homology domain

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
28 74 2m7n (A) 86 127 48.00 0.6 0.89 0.811158 0.34
44 115 4tql (A) 121 195 21.00 0.54 0.03 0.927142 -1.58
2 400 5h7c (A) 8 403 14.00 0.0004 0.01 1.04624 -0.48
112 238 5hmo (A) 806 925 35.00 0.0019 0.06 0.483284 -0.09
182 431 4tql (A) 10 230 27.00 0.0058 0.95 0.789394 -0.44
201 404 5cwm (A) 4 225 28.00 0.026 0.7 0.80589 -0.91
222 410 4uos (A) 1 184 14.00 0 0.19 0.640786 -1.64

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_130109)

Species Accession Gene Product
Drosophila melanogaster Dmel_CG7352   CG7352 gene product from transcript CG7352-RC
Giardia lamblia GL50803_16267   Hypothetical protein
Homo sapiens ENSG00000138587   meiosis-specific nuclear structural 1
Leishmania braziliensis LbrM.30.3160   hypothetical protein, conserved
Leishmania donovani LdBPK_303210.1   Tumour suppressor, Mitostatin, putative
Leishmania infantum LinJ.30.3210   hypothetical protein, conserved
Leishmania major LmjF.30.3170   hypothetical protein, conserved
Leishmania mexicana LmxM.29.3170   hypothetical protein, conserved
Mus musculus 17427   meiosis-specific nuclear structural protein 1
Neospora caninum NCLIV_049280   Trichohyalin, related
Plasmodium berghei PBANKA_1409600   meiosis-specific nuclear structural protein 1, putative
Plasmodium falciparum PF3D7_1311100   meiosis-specific nuclear structural protein 1, putative
Plasmodium knowlesi PKNH_1411500   meiosis-specific nuclear structural protein 1, putative
Plasmodium vivax PVX_122385   hypothetical protein, conserved
Plasmodium yoelii PY02801   unnamed protein product, putative
Schistosoma japonicum Sjp_0034740   Meiosis-specific nuclear structural protein 1, putative
Schistosoma mansoni Smp_068350   hypothetical protein
Schmidtea mediterranea mk4.005183.04  
Trypanosoma brucei gambiense Tbg972.6.4300   hypothetical protein, conserved
Trypanosoma brucei Tb927.6.4520   Tumour suppressor, Mitostatin, putative
Trypanosoma congolense TcIL3000_0_03160   Tumour suppressor, Mitostatin, putative
Trypanosoma congolense TcIL3000_6_3960   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.509167.60   Tumour suppressor, Mitostatin, putative
Toxoplasma gondii TGME49_212075   hypothetical protein
Trichomonas vaginalis TVAG_123230   Inner centromere protein, putative
Trichomonas vaginalis TVAG_187340   ubiquitin-protein ligase BRE1A, putative

Essentiality

Tb927.6.4520 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.4520 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.4520 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.4520 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.4520 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1409600 Plasmodium berghei Dispensable plasmo
TGME49_212075 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.6.4520 (Trypanosoma brucei), Tumour suppressor, Mitostatin, putative
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