Detailed view for Tb11.v5.0271

Basic information

TDR Targets ID: 12054
Trypanosoma brucei, trypanin, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.8622 | Length (AA): 453 | MW (Da): 53924 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13851   Growth-arrest specific micro-tubule binding

Gene Ontology

Mouse over links to read term descriptions.
GO:0048870   cell motility involved in cell locomotion  
GO:0031514   motile secondary cilium  

Metabolic Pathways

African trypanosomiasis (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
94 323 4tql (A) 9 231 20.00 0.01 0.97 0.797326 -0.91
169 324 5nmo (A) 188 1010 15.00 0 0.18 0.465671 -0.72
187 370 4uos (A) 6 179 13.00 0 0.18 0.526481 -1.29
275 370 4mh6 (A) 29 131 34.00 0.14 0.4 0.554521 -0.61

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129322)

Species Accession Gene Product
Drosophila melanogaster Dmel_CG14271   Growth arrest specific protein 8
Echinococcus granulosus EgrG_001122500   growth arrest specific protein 8
Echinococcus granulosus EgrG_000191500   growth arrest specific protein 8
Echinococcus multilocularis EmuJ_000191500   growth arrest specific protein 8
Echinococcus multilocularis EmuJ_001122500   growth arrest specific protein 8
Giardia lamblia GL50803_16540   Dynein regulatory complex
Homo sapiens ENSG00000141013   growth arrest-specific 8
Leishmania braziliensis LbrM.34.1720   trypanin-like protein
Leishmania braziliensis LbrM.35.2110   T-lymphocyte triggering factor, putative
Leishmania donovani LdBPK_351800.1   nexin-dynein regulatory complex 4
Leishmania donovani LdBPK_362000.1   T-lymphocyte triggering factor, putative
Leishmania infantum LinJ.36.2000   T-lymphocyte triggering factor, putative
Leishmania infantum LinJ.35.1800   trypanin-like protein
Leishmania major LmjF.36.1910   T-lymphocyte triggering factor, putative
Leishmania major LmjF.35.1810   trypanin-like protein
Leishmania mexicana LmxM.36.1910   T-lymphocyte triggering factor, putative
Leishmania mexicana LmxM.34.1810   trypanin-like protein
Mus musculus ENSMUSG00000040220   growth arrest specific 8
Neospora caninum NCLIV_062430   growth-arrest-specific protein 8, putative
Plasmodium berghei PBANKA_1123800   GAS8-like protein, putative
Plasmodium falciparum PF3D7_0624900   GAS8-like protein, putative
Plasmodium knowlesi PKNH_1125300   GAS8-like protein, putative
Plasmodium vivax PVX_114350   hypothetical protein, conserved
Schistosoma japonicum Sjp_0047480   Growth arrest-specific protein 8, putative
Schistosoma mansoni Smp_045650   Intersectin-1 (EH and SH3 domains protein 1)
Schmidtea mediterranea mk4.028132.00   Growth arrest-specific protein 8
Schmidtea mediterranea mk4.023298.00   Growth arrest-specific protein 8
Schmidtea mediterranea mk4.021916.00   Growth arrest-specific protein 8
Schmidtea mediterranea mk4.019464.00  
Trypanosoma brucei gambiense Tbg972.10.7750   trypanin, putative
Trypanosoma brucei gambiense Tbg972.9.9310   trypanin-related protein, putative
Trypanosoma brucei Tb11.v5.0271   trypanin, putative
Trypanosoma brucei Tb927.9.15050   nexin-dynein regulatory complex 4
Trypanosoma congolense TcIL3000_9_6270   nexin-dynein regulatory complex 4
Trypanosoma cruzi TcCLB.507641.210   T-lymphocyte triggering factor, putative
Trypanosoma cruzi TcCLB.510763.100   trypanin-like protein
Toxoplasma gondii TGME49_217650   hypothetical protein
Toxoplasma gondii TGME49_248225   PF2 arrest specific protein 8/11, putative
Trichomonas vaginalis TVAG_221750   Growth-arrest-specific protein, putative
Trichomonas vaginalis TVAG_094850   Growth-arrest-specific protein, putative

Essentiality

Tb11.v5.0271 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.244.2800 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.244.2800 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.244.2800 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.244.2800 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.6350 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.6350 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.6350 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.10.6350 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_217650 Toxoplasma gondii Probably non-essential sidik
TGME49_248225 Toxoplasma gondii Probably non-essential sidik
TGME49_217650 Toxoplasma gondii Essentiality uncertain sidik
TGME49_248225 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb11.v5.0271 (Trypanosoma brucei), trypanin, putative
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