Detailed view for Tb927.11.12080

Basic information

TDR Targets ID: 12070
Trypanosoma brucei, N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.974 | Length (AA): 252 | MW (Da): 28604 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 1

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02585   GlcNAc-PI de-N-acetylase

Gene Ontology

Mouse over links to read term descriptions.
GO:0000225   N-acetylglucosaminylphosphatidylinositol deacetylase activity  
GO:0016021   integral to membrane  
GO:0006506   GPI anchor biosynthetic process  

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128077)

Species Accession Gene Product
Arabidopsis thaliana AT2G27340   N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein
Arabidopsis thaliana AT3G58130   N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein
Babesia bovis BBOV_III007080   N-acetylglucosaminyl-phosphatidylinositol de-n-acetylase, putative
Brugia malayi Bm1_48430   Uncharacterized LmbE-like protein, COG2120 containing protein
Candida albicans CaO19.4903   N-acetylglucosaminylphosphatidylinositol deacetylase
Candida albicans CaO19.12368   N-acetylglucosaminylphosphatidylinositol deacetylase
Caenorhabditis elegans CELE_Y52B11C.1   Protein Y52B11C.1
Cryptosporidium parvum cgd7_700   N-acetylglucosaminyl-phosphatidylinositolde-N-acetylase
Dictyostelium discoideum DDB_G0293136   phosphatidylinositol glycan, class L
Drosophila melanogaster Dmel_CG4433   CG4433 gene product from transcript CG4433-RD
Echinococcus granulosus EgrG_000245500   N acetylglucosaminyl phosphatidylinositol
Entamoeba histolytica EHI_015270   N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase, putative
Echinococcus multilocularis EmuJ_000245500   N acetylglucosaminyl phosphatidylinositol
Giardia lamblia GL50803_14236   hypothetical protein
Homo sapiens ENSG00000108474   phosphatidylinositol glycan anchor biosynthesis, class L
Leishmania braziliensis LbrM.09.0060   N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase
Leishmania donovani LdBPK_090060.1   N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase
Leishmania infantum LinJ.09.0060   N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase
Leishmania major LmjF.09.0040   N-acetyl-D-acetylglucosaminylphosphatidylinositol deacetylase
Leishmania mexicana LmxM.09.0040   N-acetylglucosaminyl-phosphatidylinositol deacetylase
Loa Loa (eye worm) LOAG_01808   phosphatidylinositol glycan anchor biosynthesis protein
Mus musculus 327942   phosphatidylinositol glycan anchor biosynthesis, class L
Mycobacterium tuberculosis Rv0323c   Conserved hypothetical protein
Neospora caninum NCLIV_028260   hypothetical protein
Oryza sativa 4337416   Os04g0678800
Plasmodium berghei PBANKA_1123600   N-acetylglucosaminylphosphatidylinositol deacetylase, putative
Plasmodium falciparum PF3D7_0624700   N-acetylglucosaminylphosphatidylinositol deacetylase, putative
Plasmodium vivax PVX_114344   N-acetylglucosaminylphosphatidylinositol deacetylase, putative
Plasmodium yoelii PY06379   probable n-acetylglucosaminyl-phosphatidylinositol de-n-acetylase
Saccharomyces cerevisiae YMR281W   Gpi12p
Schistosoma japonicum Sjp_0304740   ko:K03434 N-acetylglucosaminylphosphatidylinositol deacetylase [EC3.5.1.89], putative
Schistosoma mansoni Smp_035080   N-acetylglucosaminyl-phosphatidylinositol de-n-acetylase
Trypanosoma brucei gambiense Tbg972.11.13520   N-acetylglucosaminyl-phosphatidylinositol deacetylase, putative
Trypanosoma brucei Tb927.11.12080   N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase
Trypanosoma congolense TcIL3000.11.12710   N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase
Trypanosoma cruzi TcCLB.504005.20   N-Acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase, putative
Trypanosoma cruzi TcCLB.511481.40   N-Acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase, putative
Toxoplasma gondii TGME49_258110   N-acetylglucosaminylphosphatidylinositol deacetylase
Toxoplasma gondii TGME49_258100   TPR repeat region protein
Theileria parva TP04_0615   N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase, putative

Essentiality

Tb927.11.12080 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu326 Mycobacterium tuberculosis non-essential nmpdr
Tb11.01.3900 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.3900 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.3900 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.3900 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
YMR281W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1123600 Plasmodium berghei Essential plasmo
TGME49_258110 Toxoplasma gondii Probably essential sidik
TGME49_258100 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) single cell organism (CARO:0000064) bloodstream stage (PLO:0040) inferred from loss-of-function mutant phenotype (ECO:0000016) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 12364327
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) single cell organism (CARO:0000064) bloodstream stage (PLO:0040) inferred from bioassay (ECO:0000094) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: Drug: GlcNCONH2-beta-PI; Drug: GlcNCONH2-(2-O-octyl)-PI. . References: 11432820
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.11.12080 (Trypanosoma brucei), N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase
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