Detailed view for Tb927.8.6640

Basic information

TDR Targets ID: 12111
Trypanosoma brucei, Male sterility protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.9921 | Length (AA): 604 | MW (Da): 67499 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03015   Male sterility protein
PF07993   Male sterility protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0080019   GO:fatty-acyl-CoA reductase (alcohol-forming) activity  

GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
33 346 4w4t (A) 1149 1413 30.00 0.000000046 1 0.631305 0.22
33 175 1oc2 (A) 5 115 37.00 0.021 0.95 0.294267 0.2
392 423 2xdj (A) 28 59 19.00 0 0 0.466582 -2.36

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127504)

Species Accession Gene Product
Arabidopsis thaliana AT3G11980   fatty acyl-CoA reductase 2
Arabidopsis thaliana AT3G44550   fatty acyl-CoA reductase 5
Arabidopsis thaliana AT3G44560   fatty acyl-CoA reductase 8
Arabidopsis thaliana AT3G44540   fatty acyl-CoA reductase 4
Arabidopsis thaliana AT4G33790   fatty acyl-CoA reductase CER4
Arabidopsis thaliana AT5G22500   fatty acyl-CoA reductase 1
Arabidopsis thaliana AT3G56700   fatty acyl-CoA reductase 6
Brugia malayi Bm1_37285   Male sterility protein
Caenorhabditis elegans CELE_Y71H10A.2   Protein FARD-1
Dictyostelium discoideum DDB_G0289081   hypothetical protein
Drosophila melanogaster Dmel_CG12268   CG12268 gene product from transcript CG12268-RB
Drosophila melanogaster Dmel_CG5065   CG5065 gene product from transcript CG5065-RA
Drosophila melanogaster Dmel_CG1443   waterproof
Homo sapiens ENSG00000197601   fatty acyl CoA reductase 1
Homo sapiens ENSG00000064763   fatty acyl CoA reductase 2
Leishmania braziliensis LbrM.24.1700   hypothetical protein, conserved
Leishmania donovani LdBPK_241710.1   Male sterility protein, putative
Leishmania infantum LinJ.24.1710   hypothetical protein, conserved
Leishmania major LmjF.24.1640   hypothetical protein, conserved
Leishmania mexicana LmxM.24.1640   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_07175   male sterility protein
Mus musculus ENSMUSG00000030759   fatty acyl CoA reductase 1
Mus musculus ENSMUSG00000030303   fatty acyl CoA reductase 2
Neospora caninum NCLIV_021430   hypothetical protein
Oryza sativa 4335559   Os04g0353600
Oryza sativa 4335561   Os04g0354400
Oryza sativa 4331746   Os03g0167600
Oryza sativa 4335562   Os04g0354600
Schmidtea mediterranea mk4.025342.00  
Schmidtea mediterranea mk4.004374.05  
Trypanosoma brucei gambiense Tbg972.8.6810   hypothetical protein, conserved
Trypanosoma brucei Tb927.8.6640   Male sterility protein, putative
Trypanosoma congolense TcIL3000_0_09330   Male sterility protein, putative
Trypanosoma cruzi TcCLB.510721.10   Male sterility protein, putative
Trypanosoma cruzi TcCLB.511909.10   Male sterility protein, putative
Toxoplasma gondii TGME49_203580   NAD-binding domain 4 domain-containing protein

Essentiality

Tb927.8.6640 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.6640 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.6640 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.6640 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.6640 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_203580 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.8.6640 (Trypanosoma brucei), Male sterility protein, putative
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