Detailed view for Tb927.8.5810

Basic information

TDR Targets ID: 12224
Trypanosoma brucei, mitochondrial carrier protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.5712 | Length (AA): 285 | MW (Da): 30749 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 2

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00153   Mitochondrial carrier protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0005743   mitochondrial inner membrane  
GO:0005488   binding  
GO:0006810   transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
24 202 4c9g (A) 127 309 28.00 0.00000018 0.11 0.700516 0.67

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128339)

Species Accession Gene Product
Arabidopsis thaliana AT5G46800   mitochondrial carnitine/acylcarnitine carrier-like protein
Brugia malayi Bm1_36285   Protein dif-1
Candida albicans CaO19.10130   likely mitochondrial carnitine transport protein similar to S. cerevisiae CRC1 (YOR100C)
Candida albicans CaO19.2599   likely mitochondrial carnitine transport protein similar to S. cerevisiae CRC1 (YOR100C)
Caenorhabditis elegans CELE_F49E8.5   Protein DIF-1
Dictyostelium discoideum DDB_G0293556   mitochondrial carnitine/acylcarnitine carrier protein
Dictyostelium discoideum DDB_G0290913   mitochondrial substrate carrier family protein
Drosophila melanogaster Dmel_CG3057   congested-like trachea
Drosophila melanogaster Dmel_CG3476   CG3476 gene product from transcript CG3476-RA
Echinococcus granulosus EgrG_000775200   mitochondrial carnitine:acylcarnitine carrier
Echinococcus multilocularis EmuJ_000775200   mitochondrial carnitine:acylcarnitine carrier
Homo sapiens ENSG00000178537   solute carrier family 25 (carnitine/acylcarnitine translocase), member 20
Leishmania braziliensis LbrM.16.0210   mitochondrial ornithine carrier protein-like protein
Leishmania donovani LdBPK_160210.1   mitochondrial ornithine carrier protein-like protein
Leishmania infantum LinJ.16.0210   mitochondrial ornithine carrier protein-like protein
Leishmania major LmjF.16.0200   mitochondrial ornithine carrier protein-like protein
Leishmania mexicana LmxM.16.0200   mitochondrial ornithine carrier protein-like protein
Loa Loa (eye worm) LOAG_03066   dif-1
Mus musculus ENSMUSG00000032602   solute carrier family 25 (mitochondrial carnitine/acylcarnitine translocase), member 20
Oryza sativa 4349476   Os10g0573800
Oryza sativa 4349475   Os10g0573700
Saccharomyces cerevisiae YOR100C   Crc1p
Schistosoma japonicum Sjp_0052160   ko:K03454 mitochondrial carrier protein, MC family, putative
Schistosoma mansoni Smp_064150   mitochondrial carnitine/acylcarnitine carrier protein-related
Schmidtea mediterranea mk4.000566.03   Mitochondrial carnitine/acylcarnitine carrier protein
Schmidtea mediterranea mk4.002387.00   Mitochondrial carnitine/acylcarnitine carrier protein CACL
Trypanosoma brucei gambiense Tbg972.8.5810   mitochondrial carrier protein, putative,mitochondrial carnitine/acylcarnitine carrier protein
Trypanosoma brucei gambiense Tbg972.11.5970   mitochondrial carrier protein, putative
Trypanosoma brucei Tb927.11.5290   mitochondrial carrier protein
Trypanosoma brucei Tb927.8.5810   mitochondrial carrier protein
Trypanosoma congolense TcIL3000.11.5540   mitochondrial carrier protein
Trypanosoma congolense TcIL3000_8_5610   mitochondrial carrier protein
Trypanosoma congolense TcIL3000_0_57240   mitochondrial carrier protein
Trypanosoma congolense TcIL3000.11.5530   mitochondrial carrier protein, putative
Trypanosoma cruzi TcCLB.509109.114   mitochondrial carrier protein, putative
Trypanosoma cruzi TcCLB.504217.19   mitochondrial carrier protein, putative
Trypanosoma cruzi TcCLB.506221.40   mitochondrial carrier protein, putative
Trypanosoma cruzi TcCLB.504131.190   mitochondrial carrier protein, putative
Trypanosoma cruzi TcCLB.510603.90   mitochondrial carrier protein, putative
Trypanosoma cruzi TcCLB.504125.50   mitochondrial carrier protein, putative

Essentiality

Tb927.8.5810 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.2960 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.2960 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.2960 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.2960 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.8.5810 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.5810 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.5810 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.8.5810 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F49E8.5 Caenorhabditis elegans embryonic lethal wormbase
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens solute carrier family 25 (carnitine/acylcarnitine translocase), member 20 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.8.5810 (Trypanosoma brucei), mitochondrial carrier protein
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