Detailed view for Tb927.5.3600

Basic information

TDR Targets ID: 12264
Trypanosoma brucei, ATP-dependent DEAD/H RNA helicase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.3455 | Length (AA): 948 | MW (Da): 103619 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00270   DEAD/DEAH box helicase
PF00271   Helicase conserved C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0006139   nucleobase, nucleoside, nucleotide and nucleic acid metabolic process  
GO:0005575   cellular_component  
GO:0008026   ATP-dependent helicase activity  
GO:0005524   ATP binding  
GO:0004386   helicase activity  
GO:0003676   nucleic acid binding  

Metabolic Pathways

Spliceosome (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127962)

Species Accession Gene Product
Arabidopsis thaliana AT3G09620   DEAD-box ATP-dependent RNA helicase 45
Arabidopsis thaliana AT1G20920   DEAD Box RNA Helicase RCF1
Babesia bovis BBOV_I004920   DEAD/DEAH box helicase and helicase conserved C-terminal domain containing protein
Brugia malayi Bm1_39745   KIAA0801 protein
Candida albicans CaO19.14123   spliceosomal RNA helicase
Candida albicans CaO19.6831   spliceosomal RNA helicase
Caenorhabditis elegans CELE_F53H1.1   Protein F53H1.1
Cryptosporidium hominis Chro.80099   ATP-dependent RNA helicase
Cryptosporidium parvum cgd8_800   Prp5p C terminal KH. eIF4A-1-family RNA SFII helicase
Dictyostelium discoideum DDB_G0275443   DEAD/DEAH box helicase
Drosophila melanogaster Dmel_CG6227   CG6227 gene product from transcript CG6227-RA
Echinococcus granulosus EgrG_001058000   ATP dependent RNA helicase DDX46
Entamoeba histolytica EHI_013960   DEAD/DEAH box helicase, putative
Echinococcus multilocularis EmuJ_001058000   ATP dependent RNA helicase DDX46
Homo sapiens ENSG00000145833   DEAD (Asp-Glu-Ala-Asp) box polypeptide 46
Leishmania braziliensis LbrM.08.0080   ATP-dependent DEAD/H RNA helicase, putative
Leishmania donovani LdBPK_080080.1   ATP-dependent DEAD/H RNA helicase, putative
Leishmania infantum LinJ.08.0080   ATP-dependent DEAD/H RNA helicase, putative
Leishmania major LmjF.08.0080   ATP-dependent DEAD/H RNA helicase, putative
Leishmania mexicana LmxM.08.0080   ATP-dependent DEAD/H RNA helicase, putative
Loa Loa (eye worm) LOAG_00736   RNA helicase
Mus musculus ENSMUSG00000021500   DEAD (Asp-Glu-Ala-Asp) box polypeptide 46
Neospora caninum NCLIV_005180   hypothetical protein
Oryza sativa 4344716   Os08g0159900
Onchocerca volvulus OVOC5073  
Plasmodium berghei PBANKA_1108300   pre-mRNA-processing ATP-dependent RNA helicase PRP5, putative
Plasmodium falciparum PF3D7_0508700   pre-mRNA-processing ATP-dependent RNA helicase PRP5, putative
Plasmodium knowlesi PKNH_1024900   pre-mRNA-processing ATP-dependent RNA helicase PRP5, putative
Plasmodium vivax PVX_097995   ATP-dependent RNA helicase, putative
Plasmodium yoelii PY05239   similar to RNA helicases, putative
Saccharomyces cerevisiae YBR237W   DEAD-box RNA helicase PRP5
Schistosoma japonicum Sjp_0313390   expressed protein
Schistosoma mansoni Smp_174210.1   DEAD box ATP-dependent RNA helicase
Schistosoma mansoni Smp_174210.2   DEAD box ATP-dependent RNA helicase
Schistosoma mansoni Smp_174210.3   DEAD box ATP-dependent RNA helicase
Schmidtea mediterranea mk4.004776.00   Probable ATP-dependent RNA helicase DDX46
Schmidtea mediterranea mk4.017211.00  
Schmidtea mediterranea mk4.000410.01  
Trypanosoma brucei gambiense Tbg972.5.5020   ATP-dependent DEAD/H RNA helicase, putative
Trypanosoma brucei Tb927.5.3600   ATP-dependent DEAD/H RNA helicase, putative
Trypanosoma congolense TcIL3000_0_04480   ATP-dependent DEAD/H RNA helicase, putative
Trypanosoma congolense TcIL3000_5_4040   ATP-dependent DEAD/H RNA helicase, putative
Trypanosoma cruzi TcCLB.507811.30   ATP-dependent DEAD/H RNA helicase, putative
Trypanosoma cruzi TcCLB.511649.169   ATP-dependent DEAD/H RNA helicase, putative
Toxoplasma gondii TGME49_221660   DEAD/DEAH box helicase domain-containing protein
Theileria parva TP03_0532   ATP-dependent RNA helicase, putative
Theileria parva TP03_0533   hypothetical protein
Trichomonas vaginalis TVAG_283670   DEAD box ATP-dependent RNA helicase, putative

Essentiality

Tb927.5.3600 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.3600 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.5.3600 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.3600 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.3600 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F53H1.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_F53H1.1 Caenorhabditis elegans larval arrest wormbase
CELE_F53H1.1 Caenorhabditis elegans slow growth wormbase
CELE_F53H1.1 Caenorhabditis elegans sterile wormbase
YBR237W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1108300 Plasmodium berghei Essential plasmo
TGME49_221660 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.5.3600 (Trypanosoma brucei), ATP-dependent DEAD/H RNA helicase, putative
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