Detailed view for Tb927.10.8190

Basic information

TDR Targets ID: 12348
Trypanosoma brucei, T-complex protein 1, theta subunit, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.1912 | Length (AA): 537 | MW (Da): 58081 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00118   TCP-1/cpn60 chaperonin family

Gene Ontology

Mouse over links to read term descriptions.
GO:0051082   unfolded protein binding  
GO:0005524   ATP binding  
GO:0005515   protein binding  
GO:0044267   cellular protein metabolic process  
GO:0006457   protein folding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
9 521 1q3q (A) 9 525 34.00 0 1 1.42161 -0.47
10 521 4b2t (Q) 13 528 50.00 0 1 1.49175 -0.19
10 521 4b2t (q) 1013 1528 53.00 0 1 0.938745 1.94
213 358 1ass (A) 1 148 27.00 0 1 0.699181 -0.99

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127923)

Species Accession Gene Product
Arabidopsis thaliana AT3G03960   TCP-1/cpn60 chaperonin family protein
Babesia bovis BBOV_IV011960   HSP60 fold T-complex protein 1, putative
Brugia malayi Bm1_48160   T-complex protein 1 theta subunit
Candida albicans CaO19.13518   similar to S. cerevisiae CCT8 (YJL008C) subunit of chaperonin Cct ring complex involved in cytoskeleton assembly and protein fol
Candida albicans CaO19.6099   similar to S. cerevisiae CCT8 (YJL008C) subunit of chaperonin Cct ring complex involved in cytoskeleton assembly and protein fol
Caenorhabditis elegans CELE_Y55F3AR.3   Protein CCT-8
Cryptosporidium hominis Chro.20101   hypothetical protein
Cryptosporidium parvum cgd2_900   putative T complex chaperonin
Dictyostelium discoideum DDB_G0276233   chaperonin containing TCP1 theta subunit
Drosophila melanogaster Dmel_CG8258   CG8258 gene product from transcript CG8258-RA
Echinococcus granulosus EgrG_000683800   T complex protein 1 subunit theta
Entamoeba histolytica EHI_140600   T-complex protein 1 theta subunit, putative
Echinococcus multilocularis EmuJ_000683800   T complex protein 1 subunit theta
Giardia lamblia GL50803_13500   TCP-1 chaperonin subunit theta
Homo sapiens ENSG00000156261   chaperonin containing TCP1, subunit 8 (theta)
Leishmania braziliensis LbrM.35.7280   T-complex protein 1, theta subunit, putative,chaperonin, putative
Leishmania donovani LdBPK_367240.1   T-complex protein 1, theta subunit, putative
Leishmania infantum LinJ.36.7240   T-complex protein 1, theta subunit, putative,chaperonin, putative
Leishmania major LmjF.36.6910   T-complex protein 1, theta subunit, putative,chaperonin, putative
Loa Loa (eye worm) LOAG_04849   T-complex protein 1 theta subunit
Mus musculus ENSMUSG00000025613   chaperonin containing Tcp1, subunit 8 (theta)
Neospora caninum NCLIV_012700   TCP-1/cpn60 family chaperonin, putative
Oryza sativa 4334490   Os03g0804800
Plasmodium berghei PBANKA_0310900   T-complex protein 1 subunit theta, putative
Plasmodium falciparum PF3D7_0214000   T-complex protein 1 subunit theta
Plasmodium knowlesi PKNH_0406800   T-complex protein 1 subunit theta, putative
Plasmodium vivax PVX_002835   T-complex protein 1 subunit theta, putative
Plasmodium yoelii PY03932   T-complex protein 1
Saccharomyces cerevisiae YJL008C   Cct8p
Schistosoma japonicum Sjp_0035720   ko:K09500 T-complex protein 1 subunit theta, putative
Schistosoma mansoni Smp_046090.2   chaperonin containing t-complex protein 1 theta subunit tcpq
Schistosoma mansoni Smp_046090.1   chaperonin containing t-complex protein 1 theta subunit tcpq
Schmidtea mediterranea mk4.001112.04   T-complex protein 1 subunit theta
Schmidtea mediterranea mk4.001440.00   Chaperonin-containing TCP1 theta subunit
Trypanosoma brucei gambiense Tbg972.10.10010   T-complex protein 1, theta subunit, putative,CCT-theta, putative
Trypanosoma brucei Tb927.10.8190   T-complex protein 1, theta subunit, putative
Trypanosoma congolense TcIL3000_10_7080   T-complex protein 1, theta subunit, putative
Trypanosoma cruzi TcCLB.506247.50   T-complex protein 1, theta subunit, putative
Toxoplasma gondii TGME49_287500   T complex chaperonin, putative
Theileria parva TP01_1113   chaperonin 60 kDa, putative
Trichomonas vaginalis TVAG_466720   chaperonin containing t-complex protein 1, eta subunit, tcph, putative
Trichomonas vaginalis TVAG_466730   chaperonin-60kD, ch60, putative
Trichomonas vaginalis TVAG_466660   chaperonin, putative

Essentiality

Tb927.10.8190 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.8190 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.8190 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.8190 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.8190 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y55F3AR.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y55F3AR.3 Caenorhabditis elegans larval arrest wormbase
CELE_Y55F3AR.3 Caenorhabditis elegans slow growth wormbase
CELE_Y55F3AR.3 Caenorhabditis elegans sterile wormbase
YJL008C Saccharomyces cerevisiae inviable yeastgenome
TGME49_287500 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Macaaca fascicularis T-complex protein 1 subunit beta 535 aa 29.4% 510 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.10.8190 (Trypanosoma brucei), T-complex protein 1, theta subunit, putative
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