Detailed view for Tb927.11.13980

Basic information

TDR Targets ID: 12441
Trypanosoma brucei, Outer row dynein-assembly protein 7
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.8872 | Length (AA): 440 | MW (Da): 50401 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13855   Leucine rich repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0044458   GO:motile cilium assembly  

GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
26 201 4r6f (A) 43 218 29.00 0.000021 1 0.6912 -0.28
46 150 4fho (A) 143 242 31.00 0.015 1 0.713836 -1.22
68 145 3g06 (A) 243 339 42.00 0.053 0.99 0.301473 0.88
69 127 4b8c (D) 359 418 36.00 0.56 1 0.615291 -0.38
329 439 5j0l (D) 3 119 15.00 0.67 0.15 0.485173 -1.31

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128896)

Species Accession Gene Product
Drosophila melanogaster Dmel_CG31623   defective transmitter release
Echinococcus granulosus EgrG_000177200   leucine rich repeat containing protein 50
Echinococcus multilocularis EmuJ_000177200   leucine rich repeat containing protein 50
Giardia lamblia GL50803_11885   Phosphatase 1 regulatory subunit, putative
Homo sapiens ENSG00000154099   dynein, axonemal, assembly factor 1
Leishmania braziliensis LbrM.35.5130   hypothetical protein, conserved
Leishmania braziliensis LbrM.32.0820   hypothetical protein, conserved
Leishmania donovani LdBPK_365110.1   hypothetical protein, conserved
Leishmania donovani LdBPK_320770.1   Outer row dynein-assembly protein 7
Leishmania infantum LinJ.36.5110   hypothetical protein, conserved
Leishmania infantum LinJ.32.0770   hypothetical protein, conserved
Leishmania major LmjF.32.0730   hypothetical protein, conserved
Leishmania major LmjF.36.4890   hypothetical protein, conserved
Leishmania mexicana LmxM.36.4890   hypothetical protein, conserved
Leishmania mexicana LmxM.31.0730   hypothetical protein, conserved
Mus musculus ENSMUSG00000031831   dynein, axonemal assembly factor 1
Neospora caninum NCLIV_065070   leucine rich repeat protein, putative
Neospora caninum NCLIV_033520   leucine rich repeat protein, putative
Plasmodium berghei PBANKA_1360600   leucine-rich repeat protein
Plasmodium falciparum PF3D7_1347800   leucine-rich repeat protein
Plasmodium knowlesi PKNH_1253100   leucine-rich repeat protein
Plasmodium vivax PVX_083285   leucine-rich repeat protein
Plasmodium yoelii PY06074   hypothetical protein
Schmidtea mediterranea mk4.004508.01   Dynein assembly factor 1, axonemal homolog
Trypanosoma brucei gambiense Tbg972.11.12370   hypothetical protein, conserved
Trypanosoma brucei gambiense Tbg972.11.15610   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.11040   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.13980   Outer row dynein-assembly protein 7
Trypanosoma congolense TcIL3000.11.14340   Outer row dynein-assembly protein 7
Trypanosoma congolense TcIL3000.11.11760   Leucine Rich repeats (2 copies), putative
Trypanosoma cruzi TcCLB.507841.30   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.511727.310   Outer row dynein-assembly protein 7
Trypanosoma cruzi TcCLB.508269.70   hypothetical protein, conserved
Trichomonas vaginalis TVAG_047300   protein phosphatases pp1 regulatory subunit, putative
Trichomonas vaginalis TVAG_376770   protein phosphatases pp1 regulatory subunit, putative
Trichomonas vaginalis TVAG_484580   protein phosphatases pp1 regulatory subunit, putative
Trichomonas vaginalis TVAG_179810   protein phosphatases pp1 regulatory subunit, putative
Trichomonas vaginalis TVAG_216780   protein phosphatases pp1 regulatory subunit, putative
Trichomonas vaginalis TVAG_299230   protein phosphatases pp1 regulatory subunit, putative
Trichomonas vaginalis TVAG_035130   protein phosphatases pp1 regulatory subunit, putative

Essentiality

Tb927.11.13980 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.2830 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.2830 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.2830 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb11.01.2830 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.01.5550 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.5550 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.5550 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.5550 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.11.13980 (Trypanosoma brucei), Outer row dynein-assembly protein 7
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