Detailed view for Tb927.7.6810

Basic information

TDR Targets ID: 12466
Trypanosoma brucei, EF-hand domain pair, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.8314 | Length (AA): 976 | MW (Da): 107287 | Paralog Number: 2

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13499   EF-hand domain pair

Gene Ontology

Mouse over links to read term descriptions.
GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0003674   molecular_function  
GO:0005509   calcium ion binding  

Metabolic Pathways

mRNA surveillance pathway (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
263 326 1ym0 (A) 153 210 34.00 0.12 0.05 0.367674 0.36
679 937 4mew (A) 225 481 39.00 0 1 0.801269 -0.85
680 936 4i5j (A) 189 443 41.00 0 1 0.81102 -1.01
705 871 1jf0 (A) 7 175 15.00 0 0.26 0.253807 -0.38
765 867 2ct9 (A) 23 135 32.00 0.005 0.65 0.327633 0.46

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127605)

Species Accession Gene Product
Arabidopsis thaliana AT5G28900   probable serine/threonine protein phosphatase 2A regulatory subunit B''gamma
Arabidopsis thaliana AT1G03960   calcium-binding EF-hand-containing protein
Arabidopsis thaliana AT5G28850   serine/threonine protein phosphatase 2A regulatory subunit B''beta
Arabidopsis thaliana AT5G44090   serine/threonine protein phosphatase 2A regulatory subunit B''alpha
Arabidopsis thaliana AT1G54450   calcium-binding EF-hand-containing protein
Brugia malayi Bm1_36600   EF hand family protein
Brugia malayi Bm1_11800   protein phosphatase 2A
Caenorhabditis elegans CELE_C06G1.5   Protein C06G1.5, isoform A
Cryptosporidium hominis Chro.70554   CG4733-PA
Cryptosporidium parvum cgd7_4970   protein phosphatase 2A regulatory subunit (contains a conserved version of EF hands)
Dictyostelium discoideum DDB_G0279813   hypothetical protein
Drosophila melanogaster Dmel_CG4733   CG4733 gene product from transcript CG4733-RD
Echinococcus granulosus EgrG_000875600   serine:threonine protein phosphatase 2A
Echinococcus multilocularis EmuJ_000875600   serine:threonine protein phosphatase 2A
Giardia lamblia GL50803_17538   Phosphoprotein phosphatase 2A regulatory subunit
Giardia lamblia GL50803_9894   Protein phosphatase 2A regulatory subunit, putative
Homo sapiens ENSG00000073711   protein phosphatase 2, regulatory subunit B'', alpha
Homo sapiens 102725016   serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta-like
Homo sapiens 102724991   serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta-like
Homo sapiens ENSG00000167393   protein phosphatase 2, regulatory subunit B'', beta
Leishmania braziliensis LbrM.34.3120   hypothetical protein, conserved
Leishmania braziliensis LbrM.17.0650   hypothetical protein, conserved
Leishmania donovani LdBPK_170730.1   EF-hand domain pair, putative
Leishmania donovani LdBPK_353260.1   EF-hand domain pair, putative
Leishmania infantum LinJ.35.3260   hypothetical protein, conserved
Leishmania infantum LinJ.17.0730   hypothetical protein, conserved
Leishmania major LmjF.35.3210   hypothetical protein, conserved
Leishmania major LmjF.17.0750   hypothetical protein, conserved
Leishmania mexicana LmxM.17.0750   hypothetical protein, conserved
Leishmania mexicana LmxM.34.3210   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_05562   protein phosphatase 2A
Loa Loa (eye worm) LOAG_10207   hypothetical protein
Loa Loa (eye worm) LOAG_10206   hypothetical protein
Loa Loa (eye worm) LOAG_08349   hypothetical protein
Mus musculus ENSMUSG00000043154   protein phosphatase 2, regulatory subunit B'', alpha
Mus musculus ENSMUSG00000093803   protein phosphatase 2 (formerly 2A), regulatory subunit B'', delta
Neospora caninum NCLIV_038240   hypothetical protein
Oryza sativa 4328580   Os02g0191700
Oryza sativa 4348893   Os10g0476600
Plasmodium berghei PBANKA_1132700   phosphatase 2A regulatory subunit-related protein, putative
Plasmodium falciparum PF3D7_1356400   phosphatase 2A regulatory subunit-related protein, putative
Plasmodium knowlesi PKNH_1115100   conserved Plasmodium protein, unknown function
Plasmodium vivax PVX_114800   hypothetical protein, conserved
Plasmodium yoelii PY01608   Arabidopsis thaliana At5g28850/F7P1_30
Schistosoma japonicum Sjp_0312740   Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta, putative
Schistosoma japonicum Sjp_0133710   Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha, putative
Schistosoma japonicum Sjp_0089540   ko:K01090 protein phosphatase [EC3.1.3.16], putative
Schistosoma mansoni Smp_089930   phosphatase 2a regulatory subunit-related
Schmidtea mediterranea mk4.000908.01   Phosphatase 2a regulatory subunit-related
Schmidtea mediterranea mk4.012041.00  
Schmidtea mediterranea mk4.012041.04  
Schmidtea mediterranea mk4.017609.00  
Trypanosoma brucei gambiense Tbg972.7.7950   hypothetical protein, conserved
Trypanosoma brucei gambiense Tbg972.9.7530   hypothetical protein, conserved
Trypanosoma brucei Tb11.v5.0895   hypothetical protein, conserved
Trypanosoma brucei Tb927.7.6810   EF-hand domain pair, putative
Trypanosoma brucei Tb927.9.12340   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_9_5120   EF-hand domain pair, putative
Trypanosoma cruzi TcCLB.510741.30   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510661.240   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510057.4   EF-hand domain pair, putative
Trypanosoma cruzi TcCLB.503967.4   EF-hand domain pair, putative
Trypanosoma cruzi TcCLB.508909.170   EF-hand domain pair, putative
Toxoplasma gondii TGME49_200400   hypothetical protein
Trichomonas vaginalis TVAG_070200   serine/threonine-protein phosphatase 2A 48 kDa regulatory subunit B, putative

Essentiality

Tb927.7.6810 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.3350 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.3350 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.3350 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.3350 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.7.6810 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.6810 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.6810 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.6810 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1132700 Plasmodium berghei Essential plasmo
TGME49_200400 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.7.6810 (Trypanosoma brucei), EF-hand domain pair, putative
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