Detailed view for Tb927.6.2960

Basic information

TDR Targets ID: 12488
Trypanosoma brucei, epsilon-adaptin, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.5394 | Length (AA): 1025 | MW (Da): 114338 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01602   Adaptin N terminal region
PF14807   Adaptin AP4 complex epsilon appendage platform

Gene Ontology

Mouse over links to read term descriptions.
GO:0030124   AP-4 adaptor complex  
GO:0030117   membrane coat  
GO:0005515   protein binding  
GO:0005488   binding  
GO:0015031   protein transport  
GO:0006886   intracellular protein transport  
GO:0016192   vesicle-mediated transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_130626)

Species Accession Gene Product
Arabidopsis thaliana AT1G31730   Adaptin family protein
Babesia bovis BBOV_III002850   adaptin N terminal region domain containing protein
Cryptosporidium hominis Chro.40252   hypothetical protein
Cryptosporidium parvum cgd4_2210   conserved hypothetical protein
Dictyostelium discoideum DDB_G0280427   adaptin N-terminal domain-containing protein
Homo sapiens ENSG00000081014   adaptor-related protein complex 4, epsilon 1 subunit
Mus musculus 108011   adaptor-related protein complex AP-4, epsilon 1
Neospora caninum NCLIV_018860   adaptin N terminal region domain-containing protein, putative
Oryza sativa 4324331   Os01g0916200
Plasmodium berghei PBANKA_0417300   AP-4 complex subunit epsilon, putative
Plasmodium falciparum PF3D7_0904100   AP-4 complex subunit epsilon, putative
Plasmodium knowlesi PKNH_0701800   AP-4 complex subunit epsilon, putative
Plasmodium vivax PVX_098650   AP-4 complex subunit epsilon, putative
Plasmodium yoelii PY01150   epsilon-adaptin, putative-related
Trypanosoma brucei gambiense Tbg972.6.2750   epsilon-adaptin, putative,AP-1/4 adapter complex gamma/epsilon subunit, putative
Trypanosoma brucei Tb927.6.2960   epsilon-adaptin, putative
Trypanosoma cruzi TcCLB.507023.10   epsilon-adaptin, putative
Trypanosoma cruzi TcCLB.511751.200   epsilon-adaptin, putative
Toxoplasma gondii TGME49_244290   adapter-related protein
Theileria parva TP04_0106   hypothetical protein
Trichomonas vaginalis TVAG_377810   adaptin, alpha/gamma/epsilon, putative
Trichomonas vaginalis TVAG_044240   adaptin, alpha/gamma/epsilon, putative
Trichomonas vaginalis TVAG_267030   adaptin, alpha/gamma/epsilon, putative
Trichomonas vaginalis TVAG_020230   adaptin, alpha/gamma/epsilon, putative

Essentiality

Tb927.6.2960 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.2960 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.2960 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.2960 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.2960 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0417300 Plasmodium berghei Essential plasmo
TGME49_244290 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.6.2960 (Trypanosoma brucei), epsilon-adaptin, putative
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