Detailed view for Tb927.7.210

Basic information

TDR Targets ID: 12738
Trypanosoma brucei, Proline dehydrogenase, mitochondrial
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.1684 | Length (AA): 556 | MW (Da): 63859 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01619   Proline dehydrogenase

Gene Ontology

Mouse over links to read term descriptions.
GO:0004657   proline dehydrogenase activity  
GO:0006562   proline catabolic process  
GO:0006537   glutamate biosynthetic process  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
82 335 3aw9 (A) 57 302 29.00 0.0061 0.22 0.349535 1.2
182 532 3haz (A) 138 463 19.00 0 1 0.796295 0.01
234 292 2a4j (A) 115 171 14.00 0 0.12 0.214115 -0.15
234 289 1f54 (A) 21 74 15.00 0 0.44 0.384719 -1.51

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127738)

Species Accession Gene Product
Arabidopsis thaliana AT5G38710   proline dehydrogenase 2
Arabidopsis thaliana AT3G30775   proline dehydrogenase 1
Brugia malayi Bm1_01540   proline oxidase, mitochondrial precursor
Candida albicans CaO19.4274   Proline dehydrogenase-like orf
Candida albicans CaO19.11750   Proline dehydrogenase-like orf
Caenorhabditis elegans CELE_B0513.5   Protein B0513.5
Dictyostelium discoideum DDB_G0275669   hypothetical protein
Drosophila melanogaster Dmel_CG1417   sluggish A
Homo sapiens ENSG00000250799   proline dehydrogenase (oxidase) 2
Homo sapiens ENSG00000100033   proline dehydrogenase (oxidase) 1
Homo sapiens ENSG00000277196   proline dehydrogenase 1, mitochondrial-like
Leishmania braziliensis LbrM.26.1630   proline oxidase, mitochondrial precursor-like protein
Leishmania donovani LdBPK_261590.1   Proline dehydrogenase, mitochondrial
Leishmania infantum LinJ.26.1590   proline oxidase, mitochondrial precursor-like protein
Leishmania major LmjF.26.1610   proline oxidase, mitochondrial precursor-like protein
Leishmania mexicana LmxM.26.1610   proline oxidase, mitochondrial precursor-like protein
Loa Loa (eye worm) LOAG_04084   sluggish A-PC
Mus musculus ENSMUSG00000003526   proline dehydrogenase
Mus musculus ENSMUSG00000036892   proline dehydrogenase (oxidase) 2
Neospora caninum NCLIV_022590   hypothetical protein
Oryza sativa 4349318   Os10g0550900
Onchocerca volvulus OVOC5441   Proline dehydrogenase 1, mitochondrial homolog
Saccharomyces cerevisiae YLR142W   proline dehydrogenase
Schistosoma japonicum Sjp_0200700   ko:K00318 proline dehydrogenase [EC1.5.99.8], putative
Schistosoma japonicum Sjp_0200690   ko:K00318 proline dehydrogenase [EC1.5.99.8], putative
Schistosoma mansoni Smp_170690   proline oxidase
Schmidtea mediterranea mk4.000910.03   Proline dehydrogenase 1, mitochondrial
Trypanosoma brucei gambiense Tbg972.7.90   proline oxidase, putative
Trypanosoma brucei Tb927.7.210   Proline dehydrogenase, mitochondrial
Trypanosoma congolense TcIL3000_0_07190   proline dehydrogenase
Trypanosoma cruzi TcCLB.506411.30   Proline dehydrogenase, mitochondrial
Toxoplasma gondii TGME49_202340   proline dehydrogenase

Essentiality

Tb927.7.210 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.210 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.210 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.210 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.210 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_202340 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

5 literature references were collected for this gene.

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Gene identifier Tb927.7.210 (Trypanosoma brucei), Proline dehydrogenase, mitochondrial
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