Detailed view for Tb927.4.1850

Basic information

TDR Targets ID: 12746
Trypanosoma brucei, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.1589 | Length (AA): 578 | MW (Da): 64384 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF16969   RNA-binding signal recognition particle 68

Gene Ontology

Mouse over links to read term descriptions.
GO:0005786   signal recognition particle, endoplasmic reticulum targeting  
GO:0030942   endoplasmic reticulum signal peptide binding  
GO:0008312   7S RNA binding  
GO:0005047   signal recognition particle binding  
GO:0003723   RNA binding  
GO:0006614   SRP-dependent cotranslational protein targeting to membrane  

Metabolic Pathways

Protein export (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 224 4p3g (A) 2 217 22.00 0 1 0.620253 -0.18
165 248 3lmh (B) 647 763 38.00 0.49 0.41 0.264429 0.35
287 499 4y6w (A) 0 210 14.00 0.015 0.1 0.444012 -0.22

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128493)

Species Accession Gene Product
Arabidopsis thaliana AT5G61970   signal recognition particle subunit SRP68
Brugia malayi Bm1_52915   signal recognition particle 68
Candida albicans CaO19.14096   signal recognition particle component
Candida albicans CaO19.6804   signal recognition particle component
Caenorhabditis elegans CELE_F55C5.8   Protein F55C5.8
Cryptosporidium hominis Chro.10280   signal recognition particle 68
Cryptosporidium parvum cgd1_2500   signal recognition particle SPR68
Dictyostelium discoideum DDB_G0285821   signal recognition particle 68 kDa subunit
Drosophila melanogaster Dmel_CG5064   Signal recognition particle protein 68
Echinococcus granulosus EgrG_000462800   signal recognition particle 68 kDa protein
Entamoeba histolytica EHI_161660   signal recognition particle 68 kDa protein, putative
Echinococcus multilocularis EmuJ_000462800   signal recognition particle 68 kDa protein
Homo sapiens ENSG00000167881   signal recognition particle 68kDa
Leishmania braziliensis LbrM.20.2350   hypothetical protein, conserved
Leishmania donovani LdBPK_342630.1   hypothetical protein, conserved
Leishmania infantum LinJ.34.2630   hypothetical protein, conserved
Leishmania major LmjF.34.2790   hypothetical protein, conserved
Leishmania mexicana LmxM.33.2790   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_06278   signal recognition particle 68
Mus musculus ENSMUSG00000020780   signal recognition particle 68
Neospora caninum NCLIV_028300   signal recognition particle, putative
Oryza sativa 4345269   Os08g0323000
Saccharomyces cerevisiae YPL243W   Srp68p
Schistosoma japonicum Sjp_0305340   similar to Signal recognition particle 68 kDa protein, putative
Schistosoma japonicum Sjp_0134330   ko:K03107 signal recognition particle, subunit SRP68, putative
Schistosoma mansoni Smp_157160   signal recognition particle 68 kD protein
Trypanosoma brucei gambiense Tbg972.4.1760   hypothetical protein, conserved
Trypanosoma brucei Tb927.4.1850   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_4_1510   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.504013.90   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510879.30   hypothetical protein, conserved
Toxoplasma gondii TGME49_258070   hypothetical protein
Trichomonas vaginalis TVAG_091590   signal recognition particle 68 kD protein, putative
Trichomonas vaginalis TVAG_518880   conserved hypothetical protein
Trichomonas vaginalis TVAG_583610   signal recognition particle 68 kD protein, putative

Essentiality

Tb927.4.1850 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.1850 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.4.1850 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.4.1850 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.4.1850 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F55C5.8 Caenorhabditis elegans embryonic lethal wormbase
CELE_F55C5.8 Caenorhabditis elegans larval arrest wormbase
CELE_F55C5.8 Caenorhabditis elegans larval lethal wormbase
CELE_F55C5.8 Caenorhabditis elegans sterile wormbase
YPL243W Saccharomyces cerevisiae inviable yeastgenome
TGME49_258070 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Tb927.4.1850 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Trypanosoma brucei ( 1 )

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.4.1850 (Trypanosoma brucei), hypothetical protein, conserved
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