Detailed view for Tb927.11.11650

Basic information

TDR Targets ID: 12902
Trypanosoma brucei, Centrosomal protein of 164 kDa, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.284 | Length (AA): 1006 | MW (Da): 111172 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00397   WW domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0003674   molecular_function  
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_131535)

Species Accession Gene Product
Giardia lamblia GL50803_24423   Hypothetical protein
Leishmania braziliensis LbrM.28.2590   hypothetical protein, conserved
Leishmania braziliensis LbrM.12.0530   hypothetical protein, conserved
Leishmania donovani LdBPK_120470.1   WW domain containing protein, putative
Leishmania donovani LdBPK_282560.1   WW domain containing protein, putative
Leishmania infantum LinJ.28.2560   hypothetical protein, conserved
Leishmania infantum LinJ.12.0470   hypothetical protein, conserved
Leishmania major LmjF.12.0510   hypothetical protein, conserved
Leishmania major LmjF.28.2390   hypothetical protein, conserved
Leishmania mexicana LmxM.12.0510   hypothetical protein, conserved
Leishmania mexicana LmxM.28.2390   hypothetical protein, conserved
Schistosoma mansoni Smp_135920   hypothetical protein
Trypanosoma brucei gambiense Tbg972.11.13070   hypothetical protein, conserved
Trypanosoma brucei gambiense Tbg972.1.2250  
Trypanosoma brucei Tb927.11.11650   Centrosomal protein of 164 kDa, putative
Trypanosoma brucei Tb927.1.3560   Centrosomal protein of 164 kDa, putative
Trypanosoma congolense TcIL3000.11.12260   WW domain containing protein, putative
Trypanosoma congolense TcIL3000_0_24030   WW domain containing protein, putative
Trypanosoma cruzi TcCLB.510889.270   Centrosomal protein of 164 kDa, putative
Trypanosoma cruzi TcCLB.506935.10   Centrosomal protein of 164 kDa, putative
Trypanosoma cruzi TcCLB.507527.20   Centrosomal protein of 164 kDa, putative
Trypanosoma cruzi TcCLB.435519.19   Centrosomal protein of 164 kDa, putative
Trypanosoma cruzi TcCLB.506529.590   Centrosomal protein of 164 kDa, putative
Trichomonas vaginalis TVAG_172550   paramyosin, putative
Trichomonas vaginalis TVAG_260210   synaptonemal complex protein, putative
Trichomonas vaginalis TVAG_140540   conserved hypothetical protein
Trichomonas vaginalis TVAG_359680   centrosomal protein of 135 kDa, putative
Trichomonas vaginalis TVAG_312820   conserved hypothetical protein

Essentiality

Tb927.11.11650 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.3520 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.3520 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.3520 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb11.01.3520 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.1.3560 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.1.3560 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.1.3560 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.1.3560 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.11.11650 (Trypanosoma brucei), Centrosomal protein of 164 kDa, putative
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