Detailed view for Tb927.3.1150

Basic information

TDR Targets ID: 13071
Trypanosoma brucei, Conserved hypothetical ATP binding protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.1208 | Length (AA): 280 | MW (Da): 30751 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03029   Conserved hypothetical ATP binding protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0000166   nucleotide binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 34 2rhm (A) 2 35 24.00 0 0.07 0.295329 0.41
1 248 1yrb (A) 498 240 20.00 0.028 0.78 1.20111 -0.74
6 261 5hcn (A) 6 260 28.00 0.000000000019 1 1.18859 -0.15
6 111 2bwj (A) 14 103 37.00 0.76 0.64 0.600871 0.31

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128003)

Species Accession Gene Product
Arabidopsis thaliana AT4G12790   P-loop containing nucleoside triphosphate hydrolases superfamily protein
Babesia bovis BBOV_IV007690   ATP binding protein, putative
Brugia malayi Bm1_56705   protein x 0004
Candida albicans CaO19.3130   conserved hypothetical ATP-binding protein similar to S. cerevisiae YLR243W
Candida albicans CaO19.10642   conserved hypothetical ATP-binding protein similar to S. cerevisiae YLR243W
Caenorhabditis elegans CELE_Y75B8A.14   Protein Y75B8A.14
Cryptosporidium hominis Chro.60489   ATP binding protein
Cryptosporidium parvum cgd6_4270   MinD type ATpase
Dictyostelium discoideum DDB_G0285197   GPN-loop GTPase 3
Drosophila melanogaster Dmel_CG2656   CG2656 gene product from transcript CG2656-RA
Echinococcus granulosus EgrG_000874100   ATP binding domain 1 family member C
Entamoeba histolytica EHI_042910   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000874100   ATP binding domain 1 family, member C
Giardia lamblia GL50803_8513   ATP-binding protein
Homo sapiens ENSG00000111231   GPN-loop GTPase 3
Leishmania braziliensis LbrM.25.1090   hypothetical protein, conserved
Leishmania donovani LdBPK_251430.1   hypothetical protein, conserved
Leishmania infantum LinJ.25.1430   hypothetical protein, conserved
Leishmania major LmjF.25.1390   hypothetical protein, conserved
Leishmania mexicana LmxM.25.1390   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_09501   GPN-loop GTPase 3
Mus musculus ENSMUSG00000029464   GPN-loop GTPase 3
Neospora caninum NCLIV_069420   hypothetical protein
Oryza sativa 4332772   Os03g0337700
Oryza sativa 4333905   Os03g0714400
Saccharomyces cerevisiae YLR243W   Gpn3p
Schistosoma japonicum Sjp_0035570   ko:K06883 similar to ATP binding domain 1 family, member C, putative
Schistosoma mansoni Smp_172090   hypothetical protein
Schmidtea mediterranea mk4.002684.01   GPN-loop GTPase 3
Schmidtea mediterranea mk4.004708.02   GPN-loop GTPase 3
Schmidtea mediterranea mk4.017284.01   GPN-loop GTPase 3
Schmidtea mediterranea mk4.003941.01   GPN-loop GTPase 3
Trypanosoma brucei gambiense Tbg972.3.880   hypothetical protein, conserved
Trypanosoma brucei Tb927.3.1150   Conserved hypothetical ATP binding protein, putative
Trypanosoma congolense TcIL3000_3_380   Conserved hypothetical ATP binding protein, putative
Trypanosoma cruzi TcCLB.509455.50   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.503879.30   hypothetical protein, conserved
Toxoplasma gondii TGME49_213650   ATP-binding domain 1 family protein
Theileria parva TP03_0254   ATP-binding protein, putative
Trichomonas vaginalis TVAG_127160   xpa-binding protein, putative

Essentiality

Tb927.3.1150 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.1150 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.1150 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.3.1150 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.3.1150 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y75B8A.14 Caenorhabditis elegans embryonic lethal wormbase
YLR243W Saccharomyces cerevisiae inviable yeastgenome
TGME49_213650 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.3.1150 (Trypanosoma brucei), Conserved hypothetical ATP binding protein, putative
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