Detailed view for PF3D7_1025100

Basic information

TDR Targets ID: 1319
Plasmodium falciparum, glutamine--fructose-6-phosphate aminotransferase [isomerizing], putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.1347 | Length (AA): 829 | MW (Da): 93659 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01380   SIS domain
PF13522   Glutamine amidotransferase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0006041   glucosamine metabolic process  
GO:0004360   glutamine-fructose-6-phosphate transaminase (isomerizing) activity  
GO:1901137   GO:carbohydrate derivative biosynthetic process  

GO:1901135   GO:carbohydrate derivative metabolic process  

GO:0097367   GO:carbohydrate derivative binding  

GO:0020011   apicoplast  
GO:0005529   sugar binding  
GO:0008152   metabolic process  
GO:0005975   carbohydrate metabolic process  

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
215 438 1gdo (A) 1 217 39.00 0 1 0.7 -1.68
215 828 2bpl (A) 1 607 32.00 0 1 1.26 -1.4
63 160 1air (A) 160 259 30.00 0.15 0.56 0.429215 -0.03
215 829 4amv (A) 1 608 31.00 0 1 1.19086 -0.69
470 828 2zj3 (A) 326 679 36.00 0 1 0.996052 -1.41
519 817 2v4m (A) 392 687 32.00 0 1 0.811976 -0.91
205 819 4amv (A) 1 608 32.00 0 1 1.22472 -0.92
453 807 2poc (A) 349 700 35.00 0 1 0.883655 -0.99
454 809 3tbf (A) 250 602 31.00 0 1 0.892876 -1.17
460 818 2zj3 (A) 326 679 36.00 0 1 1.00054 -1.41

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile gametocyte, merozoite, early schizont, late schizont. PlasmoDB
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, sporozoite, early ring, late ring, late trophozoite, Oocyst, Ring, Female Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 32 hs, early trophozoite, Male Gametocyte. Otto TD PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 24 hs, Sporozoite. Otto TD Zanghi G
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_126842)

Species Accession Gene Product
Arabidopsis thaliana AT3G24090   putative glucosamine-fructose-6-phosphate aminotransferase
Babesia bovis BBOV_IV000250   glucosamine--fructose-6-phosphate aminotransferase
Brugia malayi Bm1_38025   Glucosamine--fructose-6-phosphate aminotransferase, putative
Candida albicans CaO19.9186   glucosamine-6-P synthase
Candida albicans CaO19.1618   glucosamine-6-P synthase
Caenorhabditis elegans CELE_F07A11.2   Protein F07A11.2, isoform B
Caenorhabditis elegans CELE_F22B3.4   Protein F22B3.4
Cryptosporidium hominis Chro.10420   glucosamine-fructose-6-phosphate aminotransferase
Cryptosporidium parvum cgd1_3730   glucosamine-fructose-6-phosphate aminotransferase
Chlamydia trachomatis CT_816   glucosamine-fructose-6-phosphate aminotransferase
Dictyostelium discoideum DDB_G0286603   glucosamine-fructose-6-phosphate aminotransferase
Drosophila melanogaster Dmel_CG12449   Glutamine:fructose-6-phosphate aminotransferase 1
Drosophila melanogaster Dmel_CG1345   Glutamine:fructose-6-phosphate aminotransferase 2
Escherichia coli b3729   L-glutamine:D-fructose-6-phosphate aminotransferase
Echinococcus granulosus EgrG_000097800   Glutamine:fructose 6 phosphate aminotransferase
Entamoeba histolytica EHI_029560   glucosamine--fructose-6-phosphate aminotransferase, putative
Echinococcus multilocularis EmuJ_000097800   Glutamine:fructose 6 phosphate aminotransferase
Homo sapiens ENSG00000131459   glutamine-fructose-6-phosphate transaminase 2
Homo sapiens ENSG00000198380   glutamine--fructose-6-phosphate transaminase 1
Leishmania braziliensis LbrM.06.0930   glucosamine-fructose-6-phosphate aminotransferase, putative
Leishmania donovani LdBPK_060980.1   glucosamine-fructose-6-phosphate aminotransferase, putative
Leishmania infantum LinJ.06.0980   glucosamine-fructose-6-phosphate aminotransferase, putative
Leishmania major LmjF.06.0950   glucosamine-fructose-6-phosphate aminotransferase, putative
Leishmania mexicana LmxM.06.0950   glucosamine-fructose-6-phosphate aminotransferase, putative
Loa Loa (eye worm) LOAG_03315   glucosamine-fructose-6-phosphate aminotransferase
Mycobacterium leprae ML0371   Probable glucosamine-fructose-6-phosphate aminotransferase GlmS (Hexosephosphate aminotransferase) (D-fructose-6-phosphate amido
Mus musculus ENSMUSG00000020363   glutamine fructose-6-phosphate transaminase 2
Mus musculus ENSMUSG00000029992   glutamine fructose-6-phosphate transaminase 1
Mycobacterium tuberculosis Rv3436c   Probable glucosamine--fructose-6-phosphate aminotransferase [isomerizing] GlmS (hexosephosphate aminotransferase) (D-fructose-6-
Mycobacterium ulcerans MUL_0872   glucosamine--fructose-6-phosphate aminotransferase
Neospora caninum NCLIV_031610   Glucosamine--fructose-6-phosphate aminotransferase (Isomerizing) (EC 2.6.1.16), related
Oryza sativa 4351413   Os12g0131100
Onchocerca volvulus OVOC4684  
Plasmodium berghei PBANKA_0509300   glutamine--fructose-6-phosphate aminotransferase [isomerizing], putative
Plasmodium falciparum PF3D7_1025100   glutamine--fructose-6-phosphate aminotransferase [isomerizing], putative
Plasmodium knowlesi PKNH_0609600   glutamine--fructose-6-phosphate aminotransferase [isomerizing], putative
Plasmodium vivax PVX_111455   glutamine--fructose-6-phosphate aminotransferase [isomerizing], putative
Plasmodium yoelii PY00101   glucosamine-fructose-6-phosphate aminotransferase
Saccharomyces cerevisiae YKL104C   glutamine--fructose-6-phosphate transaminase (isomerizing) GFA1
Schistosoma japonicum Sjp_0034580   ko:K00820 glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [EC2.6.1.16], putative
Schistosoma mansoni Smp_136260   family C44 unassigned peptidase (C44 family)
Schmidtea mediterranea mk4.044559.02  
Schmidtea mediterranea mk4.044559.00  
Schmidtea mediterranea mk4.001185.03  
Schmidtea mediterranea mk4.001185.04  
Trypanosoma brucei gambiense Tbg972.7.6450   glucosamine-fructose-6-phosphate aminotransferase, putative
Trypanosoma brucei Tb927.7.5560   glucosamine-fructose-6-phosphate aminotransferase, putative
Trypanosoma cruzi TcCLB.510303.200   glucosamine-fructose-6-phosphate aminotransferase, putative
Toxoplasma gondii TGME49_231350   glucosamine-fructose-6-phosphate aminotransferase
Treponema pallidum TP0861   glucosamine--fructose-6-phosphate aminotransferase
Theileria parva TP02_0935   glucosamine-fructose-6-phosphate aminotransferase, putative
Trichomonas vaginalis TVAG_478320   glucosamine-fructose-6-phosphate aminotransferase, putative
Trichomonas vaginalis TVAG_011780   glucosamine-fructose-6-phosphate aminotransferase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0223   glucosamine 6-phosphate synthetase

Essentiality

PF3D7_1025100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.5560 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.5560 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.5560 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.7.5560 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b3729 Escherichia coli essential goodall
CELE_F07A11.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_F07A11.2 Caenorhabditis elegans sterile wormbase
CELE_F22B3.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_F22B3.4 Caenorhabditis elegans larval arrest wormbase
CELE_F22B3.4 Caenorhabditis elegans sterile wormbase
YKL104C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0509300 Plasmodium berghei Slow plasmo
TGME49_231350 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens glutamine--fructose-6-phosphate transaminase 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_1025100 (Plasmodium falciparum), glutamine--fructose-6-phosphate aminotransferase [isomerizing], putative
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