Detailed view for Tb927.7.3530

Basic information

TDR Targets ID: 13570
Trypanosoma brucei, Vacuolar protein 14 C-terminal Fig4p binding, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.5901 | Length (AA): 691 | MW (Da): 77549 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF11916   Vacuolar protein 14 C-terminal Fig4p binding

Gene Ontology

Mouse over links to read term descriptions.
GO:0070772   GO:PAS complex  

GO:0006661   phosphatidylinositol biosynthetic process  
GO:0005488   binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
7 668 1u6g (C) 165 889 12.00 0 0.87 1.04323 0.53
28 485 1qgr (A) 342 778 13.00 0 1 0.724008 0.38
233 371 1t0h (B) 226 401 31.00 0.84 0.47 0.353458 0.45
367 517 1ee4 (A) 137 295 11.00 0.19 0.32 0.376424 -0.87

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128499)

Species Accession Gene Product
Arabidopsis thaliana AT2G01690   VAC 14-like protein
Babesia bovis BBOV_II007720   conserved hypothetical protein
Brugia malayi Bm1_36015   SD04925p
Candida albicans CaO19.6411   similar to S. cerevisiae Vac14p, activator of lipid kinase Fab1p
Candida albicans CaO19.13769   similar to S. cerevisiae Vac14p, activator of lipid kinase Fab1p
Caenorhabditis elegans CELE_K04G2.6   Protein VACL-14
Dictyostelium discoideum DDB_G0289233   hypothetical protein
Drosophila melanogaster Dmel_CG5608   CG5608 gene product from transcript CG5608-RA
Echinococcus granulosus EgrG_000652600   protein VAC14
Echinococcus multilocularis EmuJ_000652600   protein VAC14
Homo sapiens ENSG00000103043   Vac14 homolog (S. cerevisiae)
Leishmania braziliensis LbrM.14.1640   hypothetical protein, conserved
Leishmania donovani LdBPK_141550.1   Vacuolar 14 Fab1-binding region/Vacuolar protein 14 C-terminal Fig4p binding, putative
Leishmania infantum LinJ.14.1550   hypothetical protein, conserved
Leishmania major LmjF.14.1450   hypothetical protein, conserved
Leishmania mexicana LmxM.14.1450   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_13991   hypothetical protein
Loa Loa (eye worm) LOAG_05770   hypothetical protein
Loa Loa (eye worm) LOAG_06773   hypothetical protein
Mus musculus ENSMUSG00000010936   Vac14 homolog (S. cerevisiae)
Neospora caninum NCLIV_018650   hypothetical protein
Oryza sativa 4332109   Os03g0223700
Plasmodium berghei PBANKA_1440600   VAC14 domain-containing protein, putative
Plasmodium falciparum PF3D7_1225700   VAC14 domain-containing protein, putative
Plasmodium knowlesi PKNH_1445100   VAC14 domain-containing protein, putative
Plasmodium vivax PVX_123915   hypothetical protein, conserved
Plasmodium yoelii PY05852   hypothetical protein
Saccharomyces cerevisiae YLR386W   Vac14p
Schistosoma japonicum Sjp_0111050   hypothetical protein
Schistosoma japonicum Sjp_0131330   Protein VAC14 homolog, putative
Schistosoma mansoni Smp_180010   hypothetical protein
Schistosoma mansoni Smp_172820   hypothetical protein
Schmidtea mediterranea mk4.011285.00  
Schmidtea mediterranea mk4.000870.05   Protein VAC14 homolog
Trypanosoma brucei gambiense Tbg972.7.3880   hypothetical protein, conserved
Trypanosoma brucei Tb927.7.3530   Vacuolar protein 14 C-terminal Fig4p binding, putative
Trypanosoma congolense TcIL3000_7_2750   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506443.30   Vacuolar protein 14 C-terminal Fig4p binding, putative
Toxoplasma gondii TGME49_244040   HEAT repeat-containing protein
Theileria parva TP02_0688   hypothetical protein

Essentiality

Tb927.7.3530 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.3530 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.3530 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.3530 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.7.3530 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1440600 Plasmodium berghei Essential plasmo
TGME49_244040 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Mus musculus Vac14 homolog (S. cerevisiae) Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.7.3530 (Trypanosoma brucei), Vacuolar protein 14 C-terminal Fig4p binding, putative
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