Detailed view for Tb927.10.4250

Basic information

TDR Targets ID: 13792
Trypanosoma brucei, ADP-ribosylation factor-like 2, arl2
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.7397 | Length (AA): 186 | MW (Da): 20796 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00025   ADP-ribosylation factor family

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0005525   GTP binding  
GO:0007264   small GTPase mediated signal transduction  
GO:0006886   intracellular protein transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 185 3doe (A) 2 183 63.00 0 1 1.81925 -1.18
16 178 5uf8 (A) 17 177 45.00 0 1 1.55184 -1.51
18 181 5de3 (A) 18 179 52.00 0 1 1.61522 -1.39

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128319)

Species Accession Gene Product
Arabidopsis thaliana AT2G18390   ADP-ribosylation factor-like protein 2
Babesia bovis BBOV_I002140   ADP-ribosylation factor-like protein 2, putative
Brugia malayi Bm1_08620   ADP-ribosylation factor-like protein 2
Candida albicans CaO19.10442   potential ARF-like GTP-binding protein similar to S. cerevisiae CIN4 (YMR138W) involved in beta tubulin folding
Candida albicans CaO19.2925   potential ARF-like GTP-binding protein similar to S. cerevisiae CIN4 (YMR138W) involved in beta tubulin folding
Caenorhabditis elegans CELE_F22B5.1   Protein EVL-20
Cryptosporidium hominis Chro.10030   ADP-ribosylation factor-like protein 2 (ARL2)
Cryptosporidium parvum cgd1_200   ADP-ribosylation factor-like protein 2 (ARL2), putative
Dictyostelium discoideum DDB_G0281307   ADP-ribosylation factor-like protein
Drosophila melanogaster Dmel_CG7435   ADP ribosylation factor-like 2
Echinococcus granulosus EgrG_000459100   ADP ribosylation factor 2
Echinococcus multilocularis EmuJ_000459100   ADP ribosylation factor 2
Giardia lamblia GL50803_4192   ARL2
Homo sapiens ENSG00000213465   ADP-ribosylation factor-like 2
Leishmania braziliensis LbrM.34.0170   ADP-ribosylation factor-like 2, arl2, putative
Leishmania donovani LdBPK_350130.1   ADP-ribosylation factor, putative
Leishmania infantum LinJ.35.0130   ADP-ribosylation factor-like 2, arl2, putative
Leishmania major LmjF.35.0130   ADP-ribosylation factor-like 2, arl2, putative
Leishmania mexicana LmxM.34.0130   ADP-ribosylation factor, putative
Mus musculus 56327   ADP-ribosylation factor-like 2
Neospora caninum NCLIV_013060   ADP-ribosylation factor domain-containing protein, putative
Oryza sativa 4329193   Os02g0327100
Onchocerca volvulus OVOC13476  
Plasmodium berghei PBANKA_1305900   ADP-ribosylation factor, putative
Plasmodium falciparum PF3D7_1442000   ADP-ribosylation factor, putative
Plasmodium knowlesi PKNH_1240300   ADP-ribosylation factor, putative
Plasmodium vivax PVX_118390   ADP-ribosylation-like factor, putative
Plasmodium yoelii PY00881   probable adp-ribosylation factor at2g18390
Saccharomyces cerevisiae YMR138W   Arf family GTPase CIN4
Schistosoma japonicum Sjp_0072160   ko:K07943 ADP-ribosylation factor-like 2, putative
Schistosoma mansoni Smp_164660   ADP-ribosylation factor-like 2 arl2
Schmidtea mediterranea mk4.001892.00   ADP-ribosylation factor-like protein 2
Schmidtea mediterranea mk4.046268.00  
Trypanosoma brucei gambiense Tbg972.10.5270   ADP-ribosylation factor, putative
Trypanosoma brucei Tb927.10.4250   ADP-ribosylation factor-like 2, arl2
Trypanosoma cruzi TcCLB.506295.80   ADP-ribosylation factor-like 2, arl2, putative
Trypanosoma cruzi TcCLB.511469.24   ADP-ribosylation factor-like 2, arl2, putative
Toxoplasma gondii TGME49_212950   ADP-ribosylation factor family protein 2, putative
Theileria parva TP03_0117   ADP-ribosylation factor, putative
Theileria parva TP03_0116   hypothetical protein
Trichomonas vaginalis TVAG_262220   ADP-ribosylation factor, arf, putative

Essentiality

Tb927.10.4250 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.4250 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.4250 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.4250 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.4250 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F22B5.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_F22B5.1 Caenorhabditis elegans slow growth wormbase
TGME49_212950 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.4250 (Trypanosoma brucei), ADP-ribosylation factor-like 2, arl2
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