Detailed view for Tb927.9.10970

Basic information

TDR Targets ID: 13892
Trypanosoma brucei, Serine peptidase, Clan SC, Family S09X
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.5037 | Length (AA): 370 | MW (Da): 41317 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 1

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF12146   Serine aminopeptidase, S33

Gene Ontology

Mouse over links to read term descriptions.
GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0003824   catalytic activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
54 369 2jbw (A) 57 356 17.00 0 1 1.07665 -0.4
110 225 4ypv (A) 47 165 28.00 0.045 0.87 0.670413 -0.86
142 176 5l2p (A) 78 115 43.00 0.078 0.29 0.406495 0.61
142 231 3ksr (A) 30 120 30.00 0.008 1 0.658143 -0.51

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128284)

Species Accession Gene Product
Arabidopsis thaliana AT5G20520   Bem46-family protein WAV2
Candida albicans CaO19.7627   similar to S. cerevisiae YNL320W
Cryptosporidium hominis Chro.30101   RIKEN cDNA 1110065L07
Cryptosporidium parvum cgd3_730   conserved expressed protein
Dictyostelium discoideum DDB_G0289671   hypothetical protein
Drosophila melanogaster Dmel_CG18642   CG18642 gene product from transcript CG18642-RA
Escherichia coli b2534   putative S9 family prolyl oligopeptidase
Echinococcus granulosus EgrG_001178900   abhydrolase domain containing protein 13
Entamoeba histolytica EHI_011810   hypothetical protein, conserved
Entamoeba histolytica EHI_037210   hypothetical protein
Echinococcus multilocularis EmuJ_001178900   abhydrolase domain containing protein 13
Homo sapiens ENSG00000139826   abhydrolase domain containing 13
Leishmania braziliensis LbrM.34.4000   Bem46-like serine peptidase,Serine peptidase, Clan SC, Family S09X
Leishmania donovani LdBPK_354070.1   Bem46-like serine peptidase
Leishmania infantum LinJ.35.4070   Bem46-like serine peptidase,Serine peptidase, Clan SC, Family S09X
Leishmania major LmjF.35.4020   Bem46-like serine peptidase,Serine peptidase, Clan SC, Family S09X
Leishmania mexicana LmxM.34.4020   Bem46-like serine peptidase,Serine peptidase, Clan SC, Family S09X
Mus musculus ENSMUSG00000040396   abhydrolase domain containing 13
Mycobacterium tuberculosis Rv2307c   Conserved hypothetical protein
Neospora caninum NCLIV_008960   hypothetical protein
Oryza sativa 4343867   Os07g0608300
Plasmodium berghei PBANKA_0712200   BEM46-like protein, putative
Plasmodium falciparum PF3D7_0818600   BEM46-like protein, putative
Plasmodium knowlesi PKNH_0501300   BEM46-like protein, putative
Plasmodium vivax PVX_089485   hypothetical protein, conserved
Plasmodium yoelii PY06542   unnamed protein product
Saccharomyces cerevisiae YNL320W   hypothetical protein
Schmidtea mediterranea mk4.047423.00  
Schmidtea mediterranea mk4.022783.00  
Trypanosoma brucei gambiense Tbg972.9.6490   Bem46-like serine peptidase,Serine peptidase, Clan SC, Family S09X
Trypanosoma brucei Tb927.9.10970   Serine peptidase, Clan SC, Family S09X
Trypanosoma congolense TcIL3000_9_4500   Bem46-like serine peptidase
Trypanosoma congolense TcIL3000_9_4480   Bem46-like serine peptidase
Trypanosoma cruzi TcCLB.508461.260   Serine peptidase, Clan SC, Family S09X, putative
Toxoplasma gondii TGME49_254690   phospholipase/carboxylesterase

Essentiality

Tb927.9.10970 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2343 Mycobacterium tuberculosis non-essential nmpdr
Tb09.211.2310 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.2310 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.2310 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.2310 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2534 Escherichia coli non-essential goodall
PBANKA_0712200 Plasmodium berghei Dispensable plasmo
TGME49_254690 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.9.10970 (Trypanosoma brucei), Serine peptidase, Clan SC, Family S09X
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