Detailed view for Tb927.5.590

Basic information

TDR Targets ID: 13925
Trypanosoma brucei, protein phosphatase 1, regulatory subunit, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.8359 | Length (AA): 403 | MW (Da): 45740 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13516   Leucine Rich repeat
PF13855   Leucine rich repeat
PF14580   Leucine-rich repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0008599   protein phosphatase type 1 regulator activity  
GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
40 348 1o6v (A) 85 383 29.00 0 1 1.07775 -0.22
144 261 4po4 (A) 33 170 42.00 0.0000014 1 0.690804 -0.69
173 293 4rcw (A) 362 486 28.00 0.003 1 0.734248 -1.15
184 243 3g39 (A) 30 92 40.00 0.035 0.97 0.616883 -0.32

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127611)

Species Accession Gene Product
Arabidopsis thaliana AT5G19680   leucine-rich repeat-containing protein
Babesia bovis BBOV_II003040   protein phosphatase 1, regulatory (inhibitor) subunit 7, putative
Brugia malayi Bm1_27240   hypothetical protein
Brugia malayi Bm1_46990   Leucine Rich Repeat family protein
Candida albicans CaO19.8440   regulator of protein phosphatase 1
Candida albicans CaO19.820   regulator of protein phosphatase 1
Caenorhabditis elegans CELE_T09A5.9   Protein T09A5.9
Caenorhabditis elegans CELE_C06A8.6   Protein C06A8.6
Cryptosporidium hominis Chro.60097   protein phosphatase-1 regulatory subunit 7 alpha2
Cryptosporidium parvum cgd6_750   protein phosphatase-1 regulatory subunit 7 alpha2
Dictyostelium discoideum DDB_G0284039   hypothetical protein
Drosophila melanogaster Dmel_CG5851   CG5851 gene product from transcript CG5851-RA
Echinococcus granulosus EgrG_000429200   protein phosphatase 1 regulatory subunit 7
Echinococcus granulosus EgrG_000473800   protein phosphatase 1 regulatory subunit 7
Echinococcus multilocularis EmuJ_000473800   protein phosphatase 1 regulatory subunit 7
Echinococcus multilocularis EmuJ_000429200   protein phosphatase 1 regulatory subunit 7
Homo sapiens ENSG00000115685   protein phosphatase 1, regulatory subunit 7
Leishmania braziliensis LbrM.05.1200   protein phosphatase type 1 regulator-like protein
Leishmania donovani LdBPK_051200.1   protein phosphatase type 1 regulator-like protein
Leishmania infantum LinJ.05.1200   protein phosphatase type 1 regulator-like protein
Leishmania major LmjF.05.1210   protein phosphatase type 1 regulator-like protein
Leishmania mexicana LmxM.05.1210   protein phosphatase type 1 regulator-like protein
Loa Loa (eye worm) LOAG_00522   leucine Rich Repeat family protein
Loa Loa (eye worm) LOAG_00794   leucine Rich Repeat family protein
Mus musculus 66385   protein phosphatase 1, regulatory (inhibitor) subunit 7
Neospora caninum NCLIV_038630   hypothetical protein, conserved
Oryza sativa 4328803   Os02g0230100
Onchocerca volvulus OVOC9904   Protein phosphatase 1 regulatory subunit 7 homolog
Onchocerca volvulus OVOC9155   Protein phosphatase 1 regulatory subunit 7 homolog
Plasmodium berghei PBANKA_0516600   leucine-rich repeat protein
Plasmodium falciparum PF3D7_1032800   leucine-rich repeat protein
Plasmodium knowlesi PKNH_0617600   leucine-rich repeat protein
Plasmodium vivax PVX_111085   protein phosphatases PP1 regulatory subunit sds22, putative
Plasmodium yoelii PY02599   protein phosphatase-1 regulatory subunit 7 alpha2
Saccharomyces cerevisiae YKL193C   Sds22p
Schistosoma japonicum Sjp_0101350   ko:K01768 adenylate cyclase [EC4.6.1.1], putative
Schistosoma japonicum Sjp_0305390   Protein phosphatase 1 regulatory subunit 7, putative
Schistosoma mansoni Smp_046020   protein phosphatases pp1 regulatory subunit
Schmidtea mediterranea mk4.001831.01   Protein phosphatase 1 regulatory subunit 7
Trypanosoma brucei gambiense Tbg972.5.620   protein phosphatase 1, regulatory subunit, putative
Trypanosoma brucei Tb927.5.590   protein phosphatase 1, regulatory subunit, putative
Trypanosoma congolense TcIL3000_5_290   protein phosphatase 1, regulatory subunit, putative
Trypanosoma cruzi TcCLB.507867.60   protein phosphatase 1, regulatory subunit, putative
Trypanosoma cruzi TcCLB.510629.420   protein phosphatase 1, regulatory subunit, putative
Toxoplasma gondii TGME49_267550   leucine-rich repeat protein LRR1
Theileria parva TP04_0289   hypothetical protein
Trichomonas vaginalis TVAG_356270   leucine-rich transmembrane proteins, putative
Trichomonas vaginalis TVAG_216330   leucine-rich transmembrane protein, putative
Trichomonas vaginalis TVAG_427540   leucine-rich transmembrane protein, putative
Trichomonas vaginalis TVAG_039930   leucine-rich transmembrane protein, putative
Trichomonas vaginalis TVAG_268950   conserved hypothetical protein

Essentiality

Tb927.5.590 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.590 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.590 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.590 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.590 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T09A5.9 Caenorhabditis elegans embryonic lethal wormbase
YKL193C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0516600 Plasmodium berghei Dispensable plasmo
TGME49_267550 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.5.590 (Trypanosoma brucei), protein phosphatase 1, regulatory subunit, putative
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