Detailed view for Tb927.10.8360

Basic information

TDR Targets ID: 14170
Trypanosoma brucei, Poly(A)-specific ribonuclease PARN-2
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.8274 | Length (AA): 509 | MW (Da): 58322 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04857   CAF1 family ribonuclease

Gene Ontology

Mouse over links to read term descriptions.
GO:0005634   nucleus  
GO:0003676   nucleic acid binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128150)

Species Accession Gene Product
Arabidopsis thaliana AT1G55870   poly(A)-specific ribonuclease PARN
Babesia bovis BBOV_II007020   CAF1 family ribonuclease containing protein
Caenorhabditis elegans CELE_K10C8.1   Protein K10C8.1
Homo sapiens ENSG00000140694   poly(A)-specific ribonuclease
Homo sapiens ENSG00000146453   poly(A)-specific ribonuclease (PARN)-like domain containing 1
Leishmania braziliensis LbrM.23.0650   hypothetical protein, conserved
Leishmania donovani LdBPK_230660.1   hypothetical protein, conserved
Leishmania donovani LdBPK_366630.1   poly(A)-specific ribonuclease PARN, putative
Leishmania infantum LinJ.36.6630   hypothetical protein, conserved
Leishmania infantum LinJ.23.0660   hypothetical protein, conserved
Leishmania major LmjF.36.6350   hypothetical protein, conserved
Leishmania major LmjF.23.1630   hypothetical protein, conserved
Leishmania mexicana LmxM.23.1630   hypothetical protein, conserved
Leishmania mexicana LmxM.36.6350   hypothetical protein, conserved
Mus musculus ENSMUSG00000022685   poly(A)-specific ribonuclease (deadenylation nuclease)
Mus musculus ENSMUSG00000073460   poly(A)-specific ribonuclease (PARN)-like domain containing 1
Neospora caninum NCLIV_033510   hypothetical protein
Oryza sativa 9268289   Os04g0467400
Plasmodium berghei PBANKA_1307400   poly(A)-specific ribonuclease PARN, putative
Plasmodium falciparum PF3D7_1443500   poly(A)-specific ribonuclease PARN, putative
Plasmodium knowlesi PKNH_1238800   poly(A)-specific ribonuclease PARN, putative
Plasmodium vivax PVX_118315   poly(A)-specific ribonuclease PARN, putative
Plasmodium yoelii PY01911   hypothetical protein
Schmidtea mediterranea mk4.001575.02  
Schmidtea mediterranea mk4.000807.10  
Trypanosoma brucei gambiense Tbg972.10.10190   ribonuclease, putative
Trypanosoma brucei gambiense Tbg972.8.2440   ribonuclease, putative
Trypanosoma brucei Tb927.10.8360   Poly(A)-specific ribonuclease PARN-2
Trypanosoma brucei Tb927.8.2850   Poly(A)-specific ribonuclease PARN-1
Trypanosoma congolense TcIL3000_0_41370   Poly(A)-specific ribonuclease PARN-1
Trypanosoma congolense TcIL3000_10_7200   ribonuclease, putative
Trypanosoma cruzi TcCLB.508231.74   poly(A)-specific ribonuclease PARN, putative
Trypanosoma cruzi TcCLB.506251.100   poly(A)-specific ribonuclease PARN, putative
Trypanosoma cruzi TcCLB.507221.20   poly(A)-specific ribonuclease PARN, putative
Trypanosoma cruzi TcCLB.504427.200   poly(A)-specific ribonuclease PARN, putative
Theileria parva TP02_0676   hypothetical protein, conserved

Essentiality

Tb927.10.8360 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.2850 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.2850 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.2850 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.8.2850 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.8360 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.8360 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.8360 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.8360 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1307400 Plasmodium berghei Essential plasmo
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.8360 (Trypanosoma brucei), Poly(A)-specific ribonuclease PARN-2
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